1887

Abstract

To evaluate the role of the OKT4 epitope in human herpesvirus 7 (HHV-7) infection, we studied the susceptibility to HHV-7 infection of CD4 T cells isolated from two individuals with OKT4 epitope deficiency. HHV-7-infected OKT4Leu3a T cells exhibited the characteristic cytopathic effect, reactivity with HHV-7-seropositive serum by immuno-fluorescence and down-modulation of surface CD4 in a manner similar to HHV-7-infected OKT4Leu3a T cells. A semiquantitative PCR revealed that the amounts of HHV-7 replicated in OKT4Leu3a T cells and OKT4 Leu3a T cells were not significantly different. Although it has been reported that OKT4 monoclonal antibody efficiently inhibits HHV-7 infection, the present study demonstrated that the interaction of HHV-7 with CD4 T cells does not require participation of the epitope defined by OKT4 monoclonal antibody.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-12-3103
1996-12-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/12/JV0770123103.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-12-3103&mimeType=html&fmt=ahah

References

  1. Berneman Z. N., Ablashi D. V., Li G., Eger-Fleteher M., Reitz M. S., Hung C. L., Brus I., Komaroff A. L., Gallo R. C. 1992; Human herpesvirus 7 is a T-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus. Proceedings of the National Academy of Sciences, USA 89:10552–10556
    [Google Scholar]
  2. Bach M.-A., Phan-Dinh-Tuy F., Bach J.-F., Wallach D., Biddison W. E., Sharrow S. O., Goldstein G., Kung P. C. 1981; Unusual phenotypes of human inducer T cells as measured by OKT4 and related monoclonal antibodies. Journal of Immunology 127:980–982
    [Google Scholar]
  3. Dalgleish A. G., Beverley P. C. L., Clapham P. R., Crawford D. H., Greaves M. F., Weiss R. A. 1984; The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 312:763–767
    [Google Scholar]
  4. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. 1990; Isolation of a new herpesvirus from human CD4+ T cells. Proceedings of the National Academy of Sciences, USA 87:748–752
    [Google Scholar]
  5. Furukawa M., Yasukawa M., Yakushijin Y., Fujita S. 1994; Distinct effects of human herpesvirus 6 and human herpesvirus 7 on surface molecule expression and function of CD4+ T cells. Journal of Immunology 152:5768–5775
    [Google Scholar]
  6. Hoxie J. A., Flaherty L. E., Haggarty B., Rackowski J. L. 1986; Infection of T4 lymphocytes by HTLV-III does not require expression of the OKT4 epitope. Journal of Immunology 136:361–363
    [Google Scholar]
  7. Joffe M. I., Rabson A. R. 1984; Absence of OKT4-positive lymphocytes in black African subjects. Cellular Immunology 84:453–457
    [Google Scholar]
  8. Lusso P., Secchiero P., Crowley R. W., Garzino-Demo A., Berneman Z. N., Gallo R. C. 1994; CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus. Proceedings of the National Academy of Sciences, USA 91:3872–3876
    [Google Scholar]
  9. Maddon P. J., Littman D. R., Godfrey M., Maddon D. E., Chess L., Axel R. 1985; The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family. Cell 42:93–104
    [Google Scholar]
  10. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  11. Sato M., Hayashi Y., Yoshida H., Yanagawa T., Yura Y. 1984; A family with hereditary lack of T4+ inducer/helper T cell subsets in peripheral blood lymphocytes. Journal of Immunology 132:1071–1073
    [Google Scholar]
  12. Takenaka T., Kuribayashi K., Nakamine H., Yoshikawa F., Maeda J., Kishi S., Nakauchi H., Minatogawa Y., Kido R. 1993; Autosomal codominant inheritance and Japanese incidence of deficiency of OKT4 epitope with lack of reactivity resulting from conformational change. Journal of Immunology 151:2864–2870
    [Google Scholar]
  13. Yakushijin Y., Yasukawa M., Kobayashi Y. 1991; T-cell immune response to human herpesvirus-6 in healthy adults. Microbiology and Immunology 35:655–660
    [Google Scholar]
  14. Yasukawa M., Inatsuki A., Kobayashi Y. 1989; Differential in vitro activation of CD4+CD8 and CD8+CD4 herpes simplex virus-specific human cytotoxic T cells. Journal of Immunology 143:2051–2057
    [Google Scholar]
  15. Yasukawa M., Inoue Y., Sada E., Yakushijin Y., Furukawa M., Fujita S. 1995; CD4 down-modulation by ganglioside and phorbol ester inhibits human herpesvirus 7 infection. Journal of General Virology 76:2381–2385
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-12-3103
Loading
/content/journal/jgv/10.1099/0022-1317-77-12-3103
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error