1887

Abstract

We constructred a hybrid type 1/type 3 poliovirus comprising the BC-loop of capsid protein VP1 of PV3/Finland/60212/84 and the rest derived from PV1/Mahoney, and cultured the virus in the presence of diluted rabbit antiserum to PV3/Finland/60212/84. Several strains isolated under this selection showed point mutations in the inserted type 3 poliovirus sequence but only in one case in the flanking PV1/Mahoney-derived RNA. These results indicate that, with the use of recombinant cDNA technology, it may be possible to study molecular interactions of defined regions of virus capsid proteins with neutralizing polyclonal antibodies.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-7-1861
1995-07-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/7/JV0760071861.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-7-1861&mimeType=html&fmt=ahah

References

  1. Burke K. L., Dunn G., Ferguson M., Minor P. D., Almond J. W. 1988; Antigenic chimaeras of poliovirus as potential new vaccines. Nature 322:81–82
    [Google Scholar]
  2. Diamond C. D., Jameson B. A., Bonin J., Kohara M., Abe S., Itoh H., Komatsu T., Arita M., Kuge S., Nomoto A., Osterhaus A. D. M. E., Crainic R., Wimmer E. 1985; Antigenic variation and resistance to neutralization in poliovirus type 1. Science 229:1090–1093
    [Google Scholar]
  3. Evans D. M. A., Minor P. D., Schild G. S., Almond J. W. 1993; Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3. Nature 304:459–462
    [Google Scholar]
  4. Filman D. J., Syed R., Chow M., MacAdam A., Minor P. D., Hogle J. M. 1989; Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8:1567–1579
    [Google Scholar]
  5. Fricks C. E., Icenogle J. P., Hogle J. M. 1985; Trypsin sensitivity of the Sabin strain of type 1 poliovirus: cleavage sites in virions and related particles. Journal of Virology 54:856–859
    [Google Scholar]
  6. Hogle J. M., Chow M., Filman D. J. 1985; Three-dimensional structure of poliovirus at 2.9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  7. Hovi T., Cantell K., Huovilainen A., Kinnunen E., Kuronen T., Lapinleimu K., Pöyry T., Roivainen M., Salama N., Stenvik M., Silander A., Thoden C.-J., Salminen S., Weckström P. 1986; Outbreak of poliomyelitis in Finland: widespread circulation of antigenically altered poliovirus type 3 in a vaccinated population. Lancet i:1427–1432
    [Google Scholar]
  8. Hughes P. J., Evans D. M. A., Minor P. D., Schild G. C., Almond J. W., Stanway G. 1986; The nucleotide sequence of a type 3 poliovirus isolated during a recent outbreak of poliomyelitis in Finland. Journal of General Virology 67:2093–2102
    [Google Scholar]
  9. Huovilainen A., Hovi T., Kinnunen L., Takkinen K., Ferguson M., Minor P. D. 1987; Evolution of poliovirus during an outbreak: sequential type 3 poliovirus isolates from several persons show shifts of neutralization determinants. Journal of General Virology 68:1373–1378
    [Google Scholar]
  10. Huovilainen A., Kinnunen L., Ferguson M., Hovi T. 1988; Antigenic variation among 173 strains of type 3 poliovirus isolated in Finland during the 1984 to 1985 outbreak. Journal of General Virology 69:1941–1948
    [Google Scholar]
  11. Huovilainen A., Kinnunen L., Pöyry T., Laaksonen L., Roivainen M., Hovi T. 1994; Poliovirus type 3/Saukett: antigenic and structural correlates of sequence variation in the capsid proteins. Virology 199:228–232
    [Google Scholar]
  12. Icenogle J. P., Minor P. D., Ferguson M., Hogle J. M. 1986; Modulation of humoral response to a 12-amino acid site on the poliovirus virion. Journal of Virology 60:297–301
    [Google Scholar]
  13. Kinnunen L., Huovilainen A., Pöyry T., Hovi T. 1990; Rapid molecular evolution of wild type 3 poliovirus during infection in individual hosts. Journal of General Virology 71:317–324
    [Google Scholar]
  14. Magrath D. I., Evans D. M. A., Ferguson M., Schild G. C., Minor P. D., Horaud F., Crainic R., Stenvik M., Hovi T. 1986; Antigenic and molecular properties of type 3 poliovirus responsible for an outbreak of poliomyelitis in a vaccinated population. Journal of General Virology 67:899–905
    [Google Scholar]
  15. Martin A., Wychowski C., Couderc T., Crainic R., Hogle J., Girard M. 1988; Engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice. EMBO Journal 7:2839–2847
    [Google Scholar]
  16. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  17. Minor P. D., Ferguson M., Phillips A., Magrath D. I., Huovilainen A., Hovi T. 1987; Conservation in vivo of protease cleavage sites in antigenic sites of polioviruses. Journal of General Virology 68:1857–1865
    [Google Scholar]
  18. Murray M. G., Kuhn R. J., Arita M., Kawamura N., Nomoto A., Wimmer E. 1988a; Poliovirus type 1/type 3 antigenic hybrid virus constructed in vitro elicits type 1 and type 3 neutralizing antibodies-in rabbits and monkeys. Proceedings of the National Academy of Sciences, USA 85:3203–3207
    [Google Scholar]
  19. Murray M. G., Bradley J., Yang X.-F., Wimmer E., Moss E. G., Racaniello V. R. 1988b; Poliovirus host range is determined by the eight amino acid sequence in neutralization antigenic site I. Science 241:213–215
    [Google Scholar]
  20. Page S. G., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
    [Google Scholar]
  21. Pöyry T., Kinnunen L., Kapsenberg J., Kew O., Hovi T. 1990; Type 3 poliovirus/Finland/1984 is genetically related to common Mediterranean strains. Journal of General Virology 71:2535–2541
    [Google Scholar]
  22. Roivainen M., Hovi T. 1987; Intestinal trypsin can significantly modify antigenic properties of polioviruses: implications for the use of inactivated poliovirus vaccine. Journal of Virology 61:3749–3753
    [Google Scholar]
  23. Roivainen M., Hovi T. 1988; Cleavage of VP1 and modification of antigenic site 1 of type 2 poliovirus by intestinal trypsin. Journal of Virology 62:3536–3539
    [Google Scholar]
  24. Roivainen M., Huovilainen A., Hovi T. 1990a; Antigenic modification of polioviruses by host proteolytic enzymes. Archives of Virology 111:115–126
    [Google Scholar]
  25. Roivainen M., Montagnon B., Chalumeau H., Murray M., Wimmer E., Hovi T. 1990b; Improved distribution of antigenic site specificity of poliovirus-neutralizing antibodies induced by a protease-cleaved immunogen in mice. Journal of Virology 64:559–562
    [Google Scholar]
  26. Stirck H. J., Thornton J. M. 1994; The BC loop in poliovirus coat protein VP1: an ideal acceptor site for major insertions. Protein Engineering 7:47–56
    [Google Scholar]
  27. Ukkonen P., Huovilainen A., Hovi T. 1986; Detection of poliovirus antigen by enzyme immunoassay. Journal of Clinical Microbiology 24:954–958
    [Google Scholar]
  28. van der Werf S., Bradley J., Wimmer E., Studier F. W., Dunn J. J. 1986; Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proceedings of the National Academy of Sciences, USA 83:2330–2334
    [Google Scholar]
  29. Wiegers K., Dernick R. 1992; Molecular basis of antigenic structures of poliovirus: Implications for their evolution during morphogenesis. Journal of Virology 66:4597–1600
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-7-1861
Loading
/content/journal/jgv/10.1099/0022-1317-76-7-1861
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error