1887

Abstract

The products of three open reading frames encoded by the bipartite geminiviruses have been implicated in viral spread: AC2, BV1 and BC1. Alignment of the DNA B encoded gene products, BV1 and BC1, from African cassava mosaic virus (ACMV) with six other bipartite geminiviruses showed several highly conserved regions. Specific amino acids were selected for mutagenic studies to ascertain the tolerance of the virus to change and to identify the regions within these two proteins required for normal functioning. Various mutant DNA B constructs, and a wild-type construct, were inoculated onto three host plant species with an equivalent DNA A construct. Three of the mutant constructs were infectious on and , but only two induced ACMV disease symptoms on cv. Samsun. Sequencing of the viral DNA extracted from the sap of systemically infected plants confirmed the maintenance of introduced base changes. The amino acid at position 95 on the BV1 gene product was identified as non-essential for normal functioning of the protein. The alteration of the amino acid at position 145 in BC1 demonstrated the ability of the virus to tolerate a conservative change. The lack of tolerance to other changes in amino acids has given an indication of the importance of maintaining protein structure for these proteins to function normally.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-5-1291
1995-05-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/5/JV0760051291.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-5-1291&mimeType=html&fmt=ahah

References

  1. Cherif C., Russo M. 1983; Phytological evidence of the association of a geminivirus with tomato yellow leaf curl disease in Tunisia. Phytopathologische Zeitschrift 108:221–225
    [Google Scholar]
  2. Citovsky V., Knorr D., Schuster G., Zambryski P. 1990; The p30 movement protein of tobacco mosaic virus is a single-stranded nucleic acid binding protein. Cell 60:637–647
    [Google Scholar]
  3. Citovsky V., Wong M. L., Shaw A. L., Prasad B. V. V., Zambryski P. 1992; Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids. Plant Cell 4:397–411
    [Google Scholar]
  4. Coutts R. H. A., Coffin R. S., Roberts E. J. F., Hamilton W. D. O. 1991; The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus. Journal of General Virology 72:1515–1520
    [Google Scholar]
  5. Dellaporta S. L., Wood J., Hicks J. B. 1983; A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1:19–21
    [Google Scholar]
  6. Devereux J., Haerberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Etessami P., Callis R., Ellwood S., Stanley J. 1988; Delimitation of essential genes of cassava latent virus DNA 2. Nucleic Acids Research 16:4811–1829
    [Google Scholar]
  8. Etessami P., Saunders K., Watts J., Stanley J. 1991; Mutational analysis of complementary-sense genes of African cassava mosaic virus DNA A. Journal of General Virology 72:1005–1012
    [Google Scholar]
  9. Frischmuth T., Zimmat G., Jeske H. 1990; The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178:461–468
    [Google Scholar]
  10. Haley A., Zhan X., Richardson K., Head K., Morris B. 1992; Regulation of the activities of African cassava mosaic virus promoters by the AC 1, AC2, and AC3 gene products. Virology 188:905–909
    [Google Scholar]
  11. Hamilton W. D. O., Stein V. E., Coutts R. H. A., Buck K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EM BO Journal 3:2197–2205
    [Google Scholar]
  12. Hidayat S. H., Gilbertson R. L., Hanson S. F., Morales F. J., Alquist P., Russell D. R., Maxwell D. P. 1993; Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus. Phytopathology 83:181–187
    [Google Scholar]
  13. Howarth A. J., Caton J., Bossert M., Goodman R. M. 1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proceedings of the National Academy of Sciences, USA 82:3572–3576
    [Google Scholar]
  14. Hull R., Stanley J., Briddon R. W. 1991; Geminivirus. In Classification and Nomenclature of Viruses, Fifth Report of the International Committee on Taxonomy of Viruses pp 173–177 Edited by Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. Wien: Springer-Verlag;
    [Google Scholar]
  15. Kheyr-Pour A., Bendahmane M., Matzeit V., Accotto G. P., Crespi S., Gronenborn B. 1992; Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. Nucleic Acids Research 19:6763–6769
    [Google Scholar]
  16. Kikuno R., Toh H., Hayashida H., Miyata T. 1984; Sequence similarity between putative gene products of geminiviral DNAs. Nature 308:562
    [Google Scholar]
  17. Klinkenberg F. A., Stanley J. 1990; Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana. Journal of General Virology 71:1409–1412
    [Google Scholar]
  18. Koonin E. V., Mushegian A. R., Ryabov E. V., Dolja V. V. 1991; Diverse groups of plant RNA and DNA viruses share related movement proteins that may possess charperone-like activity. Journal of General Virology 72:2895–2903
    [Google Scholar]
  19. Lazarowitz S. G., Lazdins I. B. 1991; Infectivity and complete nucleotide sequence of the cloned genomic components of a bipartite squash leaf curl geminivirus with a broad host range phenotype. Virology 180:58–69
    [Google Scholar]
  20. Maule A. J., Hull R., Donson J. 1983; The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. Journal of Virological Methods 6:215–224
    [Google Scholar]
  21. Morinaga T., Ikegami M., Miura K. 1987 In Abstracts, VII International Congress of Virology p 258 Ottawa:National Research Council of Canada;
    [Google Scholar]
  22. Morris B. A. M., Richardson K. A., Andersen M. T., Gardner R. C. 1988; Cassava latent virus infections mediated by the Ti plasmid of Agrobacterium lumefaciens containing either monomeric or dimeric viral DNA. Plant Molecular Biology 11:795–803
    [Google Scholar]
  23. Morris B., Richardson K., Eddy P., Zhan X., Haley A., Gardner R. 1991; Mutagenesis of the AC3 open reading frame of African cassava mosaic virus DNA A reduces DNA B replication and ameliorates disease symptoms. Journal of General Virology 72:1205–1213
    [Google Scholar]
  24. Mumford D. L., Thornley W. R. 1977; Location of curly top virus antigen in bean, sugarbeet, tobacco and tomato by fluorescent antibody staining. Phytopathology 67:1313–1316
    [Google Scholar]
  25. Murray E. E., Lotzer J., Eberle M. 1989; Codon usage in plant genes. Nucleic Acids Research 17:477–498
    [Google Scholar]
  26. Noueiry A. O., Lucas W. J., Gilbertson R. L. 1994; Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Cell 76:925–932
    [Google Scholar]
  27. Osman T. A. M., Hayes R. J., Buck K. W. 1992; Cooperative binding of the red clover necrotic mosaic virus to single-stranded nucleic acids. Journal of General Virology 73:223–227
    [Google Scholar]
  28. Pascal E., Goodlove P. E., Wu L. C., Lazarowitz S. G. 1993; Transgenic tobacco plants expressing the geminivirus BL1 protein exhibit symptoms of viral disease. Plant Cell 5:795–807
    [Google Scholar]
  29. Pascal E., Sanderfoot A. A., Ward B. M., Medville R., Turgeon R. R., Lazarowitz S. G. 1994; The geminivirus BR1 movement protein binds single-stranded DNA and localizes to the cell nucleus. Plant Cell 6:995–1006
    [Google Scholar]
  30. Revington G. N., Sunter G., Bisaro D. M. 1989; DNA-sequences essential for replication of the B-genome component of tomato golden mosaic virus. Plant Cell 1:985–992
    [Google Scholar]
  31. Staden R. 1990; Searching for patterns on protein and nucleic-acid sequences. Methods in Enzymology 183:193–211
    [Google Scholar]
  32. Stanley J. 1983; Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature 305:643–645
    [Google Scholar]
  33. Stanley J., Townsend R., Curson S. J. 1985; Pseudorecombinants between cloned DNAs of two isolates of cassava latent virus. Journal of General Virology 66:1055–1061
    [Google Scholar]
  34. Stanley J., Frischmuth T., Ellwood S. 1990; Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants. Proceedings of the National Academy of Sciences, USA 87:6291–6295
    [Google Scholar]
  35. Townsend R., Stanley J., Curson S. J., Short M. N. 1985; Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein. EMBO Journal 4:33–37
    [Google Scholar]
  36. Von Arnim A., Stanley J. 1992a; Determinants of tomato golden mosaic virus symptom development located on DNA B. Virology 186:286–293
    [Google Scholar]
  37. Von Arnim A., Stanley J. 1992b; Inhibition of African cassava mosaic virus systemic infection by a movement protein from the related geminivirus tomato golden mosaic virus. Virology 187:555–564
    [Google Scholar]
  38. Von Arnim A., Frischmuth T., Stanley J. 1993; Detection and possible functions of African cassava mosaic virus DNA B gene products. Virology 192:264–272
    [Google Scholar]
  39. Wolf S., Deom C. M., Beachy R. N., Lucas W. J. 1989; Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246:377–379
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-5-1291
Loading
/content/journal/jgv/10.1099/0022-1317-76-5-1291
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error