1887

Abstract

The complete nucleotide sequence of the gene encoding the protease and ICP35 capsid protein of herpes simplex virus (HSV) type 2 strain G has been determined. The gene consisits of a 1908 bp open reading frame capable of encoding 636 amino acids. Comparative nucleotide sequence analysis of the gene with published HSV-1 sequences revealed that the HSV-2 nucleic acid segment most likely encodes two distinct proteins. The first protein is the HSV-2 protease, which is required for the assembly of the herpes virus capsid. The second protein has been previously designated as ICP35 in HSV-1, and belongs to the family of herpesvirus capsid proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-4-1069
1995-04-01
2021-10-19
Loading full text...

Full text loading...

References

  1. Baum E. Z., Bebernitz G. A., Hulmes J. D., Muzithras V. P., Jones T., Gluzman Y. 1993; Expression and analysis of the human cytomegalovirus UL-80-encoded protease: identification of autoproteolytic sites. Journal of Virology 67:497–506
    [Google Scholar]
  2. Davison M. D., Rixon F. J., Davison A. J. 1992; Identification of genes encoding two capsid proteins (VP24 and VP26). of herpes simplex virus type 1. Journal of General Virology 73:2709–2713
    [Google Scholar]
  3. DiIanni C. L., Drier D. A., Deckman I. C., McCann P. J. III, Liu F., Roizman B., Colonno R. J., Cordingley M. G. 1993; Identification of the herpes simplex virus-1 protease cleavage sites by direct sequence analysis of autoproteolytic cleavage products. Journal of Biological Chemistry 368:2048–2051
    [Google Scholar]
  4. Gao M., Matusick-Kumar L., Hurlburt W., Ditusa S. F., Newcomb W. W., Brown J. C., McCann P. J., Deckman I., Colonno R. J. 1994; The protease of herpes simplex virus type 1 is essential for functional capsid formation and viral growth. Journal of Virology 68:3702–3712
    [Google Scholar]
  5. Liu F., Roizman B. 1991a; The herpes simplex virus 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate. Journal of Virology 65:5149–5156
    [Google Scholar]
  6. Liu F., Roizman B. 1991b; The promoter, transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame. Journal of Virology 65:206–212
    [Google Scholar]
  7. Liu F., Roizman B. 1992; Differentiation of multiple domains in the herpes simplex virus 1 protease encoded by the UL26 gene. Proceedings of the National Academy of Sciences, USA 89:2076–2080
    [Google Scholar]
  8. Liu F., Roizman B. 1993; Characterization of the protease and other products of the amino-terminus-proximal cleavage of the herpes simplex virus 1 UL26 protein. Journal of Virology 67:1300–1309
    [Google Scholar]
  9. Marsden H. S., Stow N. D., Preston V. G., Timbury M. C., Wilkie N. M. 1978; Physical mapping of herpes simplex virus-induced polypeptides. Journal of Virology 28:624–642
    [Google Scholar]
  10. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan S., Frame M. C., McNab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  11. Preston V. G., Coates J. A., Rixon F. J. 1983; Identification and characterization of a herpes simplex virus gene product required for encapsidation of virus DNA. Journal of Virology 45:1056–1064
    [Google Scholar]
  12. Welch A. R., Woods A. S., McNally L. M., Cotter R. J., Gibson W. 1991; A herpesvirus maturational proteinase, assemblin: identification of its gene, putative active site domain, and cleavage site. Proceedings of the National Academy of Sciences, USA 88:10792–10796
    [Google Scholar]
  13. Welch A. R., McNally L. M., Hall R. T., Gibson W. 1993; Herpesvirus proteinase: site-directed mutagenesis used to study maturational, release, and inactivation cleavage sites of precursor and to identify a possible catalytic site serine and histidine. Journal of Virology 67:7360–7372
    [Google Scholar]
  14. Zweig M., Heilman C. J., Rabin H., Hopkins R. F., Neubauer R. H., Hampar B. 1979; Production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2. Journal of Virology 32:676–678
    [Google Scholar]
  15. Zweig M., Heilman C. J., Rabin H., Hampar B. 1980; Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus. Journal of Virology 35:644–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-4-1069
Loading

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error