1887

Abstract

The envelope () gene of human T cell leukaemia virus type I (HTLV-I) was inserted into an expression vector, referred to as phMTenv, under the transcriptional control of the human metallothionein IIa gene promoter (hMT-IIa). When this vector was transiently transfected in HeLa cells treated with hMT-IIa inducers, formation of multinucleated cells was observed, indicating the expression of functional surface and transmembrane glycoproteins. Of several HeLa cell clones transfected with phMTenv together with a plasmid carrying the neomycin resistance gene and isolated after selection in G418-containing medium, mRNA was detected in only two, in the presence of hMT-IIa inducers. Viral glycoproteins were found to be weakly expressed, as detected in immunoprecipitation assays of I-surface-labelled cells. These -transfected HeLa cell clones, although unable to form syncytia when cocultivated with untransfected control HeLa cells, retained the capacity to fuse with HTLV-I-producing C91PL T cells. However, a significant decrease in their fusogenic ability was observed, after treatment with hMT-IIa inducers. Under identical experimental conditions, control HeLa cell clones stably transformed with the same plasmid, but lacking the gene, were still able to fuse with C91PL cells. These observations suggest that a post-transcriptional step in HTLV-I expression is impaired, probably leading to the establishment of superinfection interference.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-4-1021
1995-04-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/4/JV0760041021.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-4-1021&mimeType=html&fmt=ahah

References

  1. Ahmad A., Ladha A., Cohen E. A., Menezes J. 1993; Stable expression of the transfected HIV-1 env gene in a human B-cell line – characterization of gp 120-expressing clones and immunobiological studies. Virology 192:447–457
    [Google Scholar]
  2. Bird C., Gleeson P. A., RAMSAY A., Li P., McCluskey I. 1992; Stable expression of the human immunodeficiency virus type-1 envelope glycoprotein in transfected L-cells. AIDS Research and Human Retroviruses 8:1999–2009
    [Google Scholar]
  3. Carrington C. V. F., Weiss R. A., Schulz T. F. 1994; Truncated HTLV-I envelope protein, lacking the hydrophobic membrane anchor domain, is associated with cellular membranes and virions. Virology 202:61–69
    [Google Scholar]
  4. Crise B., Buonocore L., Rose J. K. 1990; CD4 is retained in the endoplasmic reticulum by the human immunodeficiency virus type 1 glycoprotein precursor. Journal of Virology 64:5585–5593
    [Google Scholar]
  5. Cullen B. R. 1992; Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological Reviews 56:375–394
    [Google Scholar]
  6. Delwart E. L., Panganiban A. T. 1989; Role of reticulo-endotheliosis virus envelope glycoprotein in superinfection interference. Journal of Virology 63:273–280
    [Google Scholar]
  7. Fukudome K., Furuse M., Imaie T., Nishimura M., Takagi S., Hinuma Y., Yoshie O. 1992; Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-l)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-l-positive T cells. Journal of Virology 66:1394–1401
    [Google Scholar]
  8. Gavalchin J., Fan N. S., Lane M. J., Papsidero L., Poiesz B. J. 1993; Identification of a putative cellular receptor for HTLV-I by a monoclonal antibody, mab 34–28. Virology 194:1–9
    [Google Scholar]
  9. Gessain A., Barin F., Vernant J. C., Gout O., Maurs L., Calender A., De The G. 1985; Antibodies to human T-lymphotropic virus type-1 in patients with tropical spastical paraparesis. Lancet ii:407–410
    [Google Scholar]
  10. Koga Y., Sasaki M., Yoshida H., Wigzell H., Kimura G., Nomoto K. 1990; Cytopathic effect determined by the amount of CD4 molecules in human cell lines expressing envelope glycoprotein of HIV. Journal of Immunology 144:94–102
    [Google Scholar]
  11. Osame M., Usuku K., Ijichi N., Amitani H., Igata A., Matsumoto M., Tara H. 1986; HTLV-I associated myelopathy: a new clinical entity. Lancet i:1031–1032
    [Google Scholar]
  12. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. 1980; Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy of Sciences, USA 77:7415–7419
    [Google Scholar]
  13. Sommerfelt M. A., Williams B. P., Clapham P. R., Solomon E., Goodfellow P. N., Weiss R. A. 1988; Human T-cell leukemia viruses use a common receptor encoded by human chromosome 17. Science 242:1557–1559
    [Google Scholar]
  14. Stevenson M., Meier C., Mann A. M., Chapman N., Wasiak A. 1988; Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4 + cells: mechanism for persistence in AIDS. Cell 53:483–496
    [Google Scholar]
  15. Vile R. G., Schulz T. F., Danos O. F., Collins M. K., Weiss R. A. 1991; A murine cell line producing HTLV-I pseudotype virions carrying a selectable marker gene. Virology 180:420–424
    [Google Scholar]
  16. Weiss R. A. 1993; Cellular receptors and viral glycoproteins involved in retrovirus entry. In The Retroviridae vol 2 pp 1–108 Edited by Levy J. A. New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-4-1021
Loading
/content/journal/jgv/10.1099/0022-1317-76-4-1021
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error