1887

Abstract

The temporal relationship between primary genital human papillomavirus (HPV) infections and the induction of antibodies against viral antigens has not been established. In order to address this question we studied a cohort of 110 women and 48 men with multiple heterosexual partners, who were followed for 220 person-years during which they made 583 visits to the sexually transmitted diseases clinic of the Amsterdam Public Health Service. At each visit spatula or brush samples from multiple anogenital and oral sites were collected for HPV DNA analysis by PCR. Serum samples were also collected and analysed for serological reactivity to peptides derived from the L1, L2 and E2 regions of HPV types 6, 16 and 18 as well as to bovine papillomavirus and HPV-16 virus-like particles. Seroconversions for at least three antigens were found among 16/158 patients. Of these, 10/16 were HPV-positive and in 5/16 cases seroconversions occurred concomitantly with the detection of HPV DNA. Analysis of participants who were HPV-negative at entry, but became HPV DNA-positive during follow-up revealed that antibodies against several HPV antigens were regularly induced at the time of a new HPV infection, in particular the IgG responses against HPV-16 virus-like particles and against the HPV-16 E2-derived peptide 245. Whereas the responses induced among the women with new HPV-16 infection tended to continuously increase, the responses among the men with any type of new HPV infection were mostly transient and disappeared during follow-up. In conclusion, we find that antibody responses to multiple viral antigens are often induced following the detection of genital HPV infection, that the type-specificity of the response is limited and that transient responses are common.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-3-529
1995-03-01
2024-11-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/3/JV0760030529.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-3-529&mimeType=html&fmt=ahah

References

  1. Cubie H. A. 1972; Serological studies in a student population prone to infection with human papilloma virus. Journal of Hygiene 70:677–690
    [Google Scholar]
  2. De Villiers E.-M. 1992; Laboratory techniques in the investigation of human papillomavirus infection. Genitourinary Medicine 68:50–54
    [Google Scholar]
  3. Dillner J. 1990; Mapping of linear epitopes of human papillomavirus type 16: the E1, E2, E4, E5, E6 and E7 open reading frames. International Journal of Cancer 46:703–711
    [Google Scholar]
  4. Dillner J. 1994; Antibody responses to defined HPV epitopes in cervical neoplasia. Papillomavirus Report 5:35–41
    [Google Scholar]
  5. Dillner J., Dillner L., Robb J., Willems J., Jones I., Lancaster W., Smith R., Lerner R. 1989; A synthetic peptide defines a serologic IgA response to a human papillomavirus-encoded nuclear antigen expressed in virus-carrying cervical neoplasia. Proceedings of the National Academy of Sciences. USA 86:3838–3841
    [Google Scholar]
  6. Dillner J., Dillner L., Utter G., Eklund C., Rotala A., Costa S., DiLuca D. 1990; Mapping of linear epitopes of human papillomavirus type 16: the L1 and L2 open reading frames. International Journal of Cancer 45:529–535
    [Google Scholar]
  7. Dillner J., Lenner P., Lehtinen M., Eklund C., Heino P., Wiklund F., Hallmans G., Stendahl U. 1994; A population-based seroepidemiological study of cervical cancer. Cancer Research 54:134–141
    [Google Scholar]
  8. Dillner L., Heino P., Moreno-Lopez J., Dillner J. 1991; Antigenic and immunogenic epitopes shared by human papillomavirus type 16 and bovine, canine and avian papillomaviruses. Journal of Virology 65:6862–6871
    [Google Scholar]
  9. Hooykaas C., van der Pligt J., van Doornum G. J. J., van der Linden M. M. D., Coutinho R. A. 1989; Heterosexuals at risk for HIV: differences between private and commercial partners in sexual behaviour and condom use. AIDS 3:525–532
    [Google Scholar]
  10. Jenison S. A., Xiu-ping Y., Valentine J. M., Koutsky L. A., Christiansen A. E., Beckmann A. M., Galloway D. A. 1990; Evidence of prevalent genital-type human papillomavirus infections in adults and children. Journal of Infectious Diseases 162:60–69
    [Google Scholar]
  11. Kirnbauer R., Taub J., Greenstone H. 1993; Efficient self assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles. Journal of Virology 67:6929–6936
    [Google Scholar]
  12. Kirnbauer R., Hubbert N. L., Wheeler C. M., Becker T. M., Lowy D. R., Schiller J. T. 1994; A virus-like particle ELISA detects serum antibodies in a majority of women infected with human papillomavirus type 16. Journal of the National Cancer Institute 86:494–499
    [Google Scholar]
  13. Krchňák V., Vágner J., Suchánkova A., Krčmář M., Ritterová L., Vonka V. 1990; Synthetic peptides derived from E7 region of human papillomavirus type 16 used as antigens in ELISA. Journal of General Virology 11:2719–2724
    [Google Scholar]
  14. Lehtinen M., Parkkonen P., Luoto H., Yla-Outinen A., Romppanen U., Rantala I., Paavonen J. 1990; Antipeptide IgA antibodies to a human papillomavirus type 16 E2 derived synthetic peptide predict the natural history of cervical HPV infections. Serono Symposia 78:509–519
    [Google Scholar]
  15. Lehtinen M., Leminen A., Paavonen J., Lehtovirta P., Hyöty H., Vestrinen E., Dillner J. 1992; Predominance of serum antibodies to synthetic peptide stemming from HPV 18 open reading frame E2 in cervical adenocarcinoma. Journal of Clinical Pathology 45:494–497
    [Google Scholar]
  16. Lehtinen M., Niemelä J., Dillner J., Parkkonen P., Nummi T., Liski E., Nieminen P., Reunala T., Paavonen J. 1993; Evaluation of serum antibody response to a newly identified B-cell epitope in the minor nucleocapsid protein L2 of human papillomavirus type 16. Clinical and Diagnostic Virology 1:153–165
    [Google Scholar]
  17. Mann V. M., Loo de Lao S., Brenes M., Brinton L. A., Rawis J. A., Green M., Reeves W. C., Rawis W. E. 1990; Occurrence of IgA and IgG antibodies to select peptides representing human papillomavirus type 16 among cervical cancer cases and controls. Cancer Research 50:7815–7819
    [Google Scholar]
  18. Mueller M., Viscidi R. P., Sun Y., Guerrero E., Hill P. M., Shah F., Bosch F. X., Munos N., Gissmann L., Shah K. V. 1992; Antibodies to HPV-16 E6 and E7 proteins as markers for HPV-16-associated invasive cervical cancer. Virology 187:508–514
    [Google Scholar]
  19. Reeves W. C., Rawls J. A., Green M., Rawls W. E. 1990; Antibodies to human papillomavirus type 16 in patients with cervical neoplasia. Lancet ii:551–552
    [Google Scholar]
  20. Rosenfeld W. D., Rose E., Vermund S. H., Schreiber K., Burk R. D. 1992; Follow-up evaluation of cervicovaginal human papillomavirus infection in adolescents. Journal of Pediatrics 121:307–311
    [Google Scholar]
  21. Schiffman M. H., Bauer H. M., Hoover R. N., Glass A. G., Cadell D. M., Rush B. B., Scott P. R., Sherman M. E., Kurman R. J., Wacholder S., Stanton C. K., Manos M. M. 1992; Epidemiological evidence that human papillomavirus infection causes most cervical intraepithelial neoplasia. Journal of the National Cancer Institute 85:958–963
    [Google Scholar]
  22. Syrjänen K., Syrjanen S. 1990; Epidemiology of human papilloma virus infections and genital neoplasia. Scandinavian Journal of Infectious Diseases Supplement 697–17
    [Google Scholar]
  23. van der Linden M. M. D., van der Velde F. W., Hooykaas C., van Doornum G. J. J., Coutinho R. A. 1990; HIV-prevalentie onder heterosexuelen met veel wisselende partners in Amsterdam. Nederlands Tijdschrift voor Geneeskunde 134:1361–1364
    [Google Scholar]
  24. van Doornum G. J. J., Hooykaas C., Juffermans L. H. J., Van der Lans S. M. G. A., van der Linden M. M. D., Coutinho R. A., Quint W. G. V. 1992; Prevalence of human papillomavirus infections among heterosexual men and women with multiple sexual partners. Journal of Medical Virology 37:13–21
    [Google Scholar]
  25. van Doornum G. J. J., Prins M., Pronk L., Coutinho R. A., Dillner J. 1994; Antibody responses to defined epitopes of human papillomavirus type 16 in relation to genital and anorectal presence of HPV DNA, a prospective study. Clinical and Diagnostic Laboratory Immunology 1:633–639
    [Google Scholar]
  26. Wikström A., Lidbrink P., Eklund C., von Krogh G., Dillner J. 1992; Levels of IgG antibodies against defined epitopes of the L1 and L2 capsid proteins of human papillomavirus type 6 are elevated in men with a history of condylomata acuminata. Journal of Clinical Microbiology 30:1795–1800
    [Google Scholar]
  27. Yaegashi N., Jenison S. A., Batra M., Galloway D. A. 1992; Human antibodies recognize multiple distinct type-specific and cross-reactive regions of the minor capsid proteins of human papillomavirus types 6 and 11. Journal of Virology 662008–2019
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-76-3-529
Loading
/content/journal/jgv/10.1099/0022-1317-76-3-529
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error