1887

Abstract

We report a novel, unusually severe cytopathic effect of interferon- (IFN-). Data concerning antibody neutralization, induction and recovery time course, CPE dose, impact on oxidative metabolic activity and 1D SDS-PAGE total cellular protein analysis are provided for preliminary characterization. This cytopathic effect appears to be linked to human papillomavirus type 16 (HPV-16) genome presence as it is markedly evident in the HPV-16-immortalized HPK-IA cell line, but is not induced in diploid keratinocytes. It is also not induced in highly malignant SiHa cells suggesting that it also requires a fairly conserved phenotype. This effect is unexpectedly not shared by IFN- pointing to a discrimination between IFN- and - signal despite the well-known sharing of a common receptor. It remains to be clarified whether this divergence, undetectable in other cellular systems, represents a direct effect of viral presence or a non-specific consequence of cellular homoeostatic disregulation induced by the papillomavirus genome.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-2-445
1995-02-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/2/JV0760020445.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-2-445&mimeType=html&fmt=ahah

References

  1. Auguet M., Grobke M., Dreiding P. 1984; Various human interferon a subclasses cross react with common receptors: their binding affinities correlate with their specific biological activities. Virology 132:211–216
    [Google Scholar]
  2. Baker C. C., Phelps W. C., Lindgren V., Braun M. J., Gonda M. A., Howley P. M. 1986; Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. Journal of Virology 61:962–971
    [Google Scholar]
  3. Baron S., Stanton G. J., Fleishmann W. R. Jr, Dianzani F. 1987; Introduction: general consideration on the interferon system. In The Interferon System: a current review to 1987, pp 1–17 Edited by Baron S., Dianzani F., Stanton G. J., Fleishmann W. G. Jr Austin: University of Texas Press;
    [Google Scholar]
  4. Delpino A., Spinsanti P., Mattel E., Mileo A. M., Vismara D., Ferrini U. 1992; Identification of a 66 kD heat shock protein (HSP) induced in M-14 human melanoma cells by severe hyperthermic treatment. Melanoma Research 2:369–375
    [Google Scholar]
  5. De Marco F., Marcante M. L. 1993; HPV-16 E6/ E7 differential transcription induced in SiHa cervical cancer cell line by interferons. Journal of Biological Regulators and Homeostatic Agents 7:15–21
    [Google Scholar]
  6. De Marco F., Di Lonardo A., Venuti A., Marcante M. L. 1991; Interferon inhibition of neoplastic phenotype in cell lines harbouring human papillomavirus sequences. Journal of Biological Regulators and Homeostatic Agents 5:65–70
    [Google Scholar]
  7. De Villiers E. M. 1989; Heterogeneity of the human papillomavirus group. Journal of Virology 63:4898–4903
    [Google Scholar]
  8. Durst M., Drazilieva Petrusevska R. T., Boukamp P., Fusenig N. E., Zur Hausen H. 1987; Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. Oncogene 1:251–256
    [Google Scholar]
  9. Fidler I. J., Heicappel R., Saiki I., Grutter M. G., Horisberger M. A., Neusch J. 1987; Direct antiproliferative effects of recombinant human interferon-a B/D hybrids on human tumor cell lines. Cancer Research 47:2020–2027
    [Google Scholar]
  10. Galloway D. A., McDougall J. K. 1989; Human papillomaviruses and carcinomas. Advances in Virus Research 37:125–171
    [Google Scholar]
  11. Khan M. A., Tolleson W. H., Gangemi D., Pirisi L. 1993; Inhibition of growth, transformation and expression of papillomavirus type 16 E7 in human keratinocytes by alpha interferons. Journal of Virology 67:3396–3403
    [Google Scholar]
  12. Kojima S., Fatutsuki T., Hatano Y., Kiode T. 1989; Purification and characterization of human interferon-alpha subtypes from Namalwa lymphoblastoid cells. Journal of Interferons Research 9:A4–8 S178
    [Google Scholar]
  13. Kraus S., Stone K. M. 1990; Management of genital infection caused by human papillomavirus. Reviews in Infectious Disease 12:620–632
    [Google Scholar]
  14. Lorincz A. T., Reid R., Jenson A. B., Cullen A., Greenberg M., Lancaster W., Kurman R. J. 1992; Human papilloma vinis infection of the cervix: relative risk associations of 15 common anogenital types. Obstetrics and Gynecology 79:328–337
    [Google Scholar]
  15. Meister A., Uzè G., Mogensen K. E., Gresser I., Tovey M. G., Grütter M., Meyer F. 1986; Biological activities and receptor binding of two human recombinant interferons and their hybrids. Journal of General Virology 67:1633–1643
    [Google Scholar]
  16. Nawa A., Nishiyama Y., Yamamoto N., Maeno K., Goto S., Tomoda Y. 1990; Selective suppression of human papillomavirus type 18 mRNA in HeLa cells by interferon. Biochemical and Biophysical Research Communications 170:793–799
    [Google Scholar]
  17. Pellegrini S., John J., Shearer M., Kerr I. M., Stark G. R. 1989; Use of a selectable marker regulated by alpha interferon to obtain mutation in signaling pathway. Molecular and Cellular Biology 9:4605–1612
    [Google Scholar]
  18. Petska S., Langer J. A., Zoon K. C., Samuel C. E. 1987; Interferons and their actions. Annual Reviews in Biochemistry 56:727–777
    [Google Scholar]
  19. Pirisi L., Yasumoto S., Feller M., Doniger J., Di Paolo A. J. 1987; Transformation of human fibroblasts and keratinocytes with human Papillomavirus type 16 DNA. Journal of Virology 61:1061–1066
    [Google Scholar]
  20. Rohlfs M., Wikenbach S., Meyer S., Rupp T., Durst M. 1991; Viral transcription in human keratinocyte cell lines immortalized by human papillomavirus type–16. Virology 183:331–342
    [Google Scholar]
  21. Ruggiero V., Latham K., Baglioni C. 1987; Cytostatic and cytotoxic activity of tumor necrosis factor on human cancer cell. Journal of Immunology 138:2711–2717
    [Google Scholar]
  22. Schneider A., Papendick U., Gissmann L., de Villiers E. M. 1987; Interferon treatment of human genital papillomavirus infection: importance of viral type. International Journal of Cancer 40:610–614
    [Google Scholar]
  23. Turek L. P., Bryne J. C., Lowy D. R., Dvoretzky I., Friedman R. M., Howley P. M. 1982; Interferon induces morphological reversion with elimination of extrachromosomal viral genomes in bovine papillomavirus-transformed mouse cells. Proceedings of the National Academy of Sciences, USA 79:7914–7918
    [Google Scholar]
  24. Uzè J., Lutfalla G., Gresser I. 1990; Genetic transfer of a functional human interferon a receptor into mouse cells: cloning and expression of its cDNA. Cell 60:225–234
    [Google Scholar]
  25. Weigent D. A., Stanton G. J., Langford R. E. L., Baron S. 1981; Virus yield-reduction assay for interferon by titration of infectious virus. Methods In Enzymology 78:346–351
    [Google Scholar]
  26. Zonn E., Miller D., Bekisz J., zur Nedded D. 1989; Chemical characterization of human lymphoblastoid interferon-alpha species. Journal of Interferons Research 9: supplement 2A4–21 S184
    [Google Scholar]
  27. Zur Hausen H. 1991; Human papillomaviruses in pathogenesis of anogenital cancer. Virology 184:9–13
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-2-445
Loading
/content/journal/jgv/10.1099/0022-1317-76-2-445
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error