1887

Abstract

Our study reveals differences in the way that tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) movement proteins (MPs) partition with cellular components separated into four fractions from different aged leaves of infected and transgenic plants. Immunoblot analyses showed that TMV and CMV MPs associated predominantly with components in the cell wall fractions from leaves of transgenic plants. In infected tissue, highest levels of TMV MP were found in the organelle fractions from young and middle-aged leaves whereas most of the CMV MP was found in the detergent wash of the cell wall fraction. These results remained consistent even when plants were doubly infected with TMV and CMV. These results imply that MPs of plant viruses from different taxonomic groups differentially associate with subcellular components and that MP produced by a viral infection is targeted to additional subcellular destinations than MP produced in transgenic plants.

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1995-12-01
2021-10-18
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References

  1. Burman A. J., Osman T. A. M., Buck K. W. 1994; Detection of the 3a protein of cucumber mosaic virus in a cell wall fraction from infected Nicotiana clevelandii plants. Journal of Phytopathology 142:317–323
    [Google Scholar]
  2. Citovsky V., McLean B. G., Zupan J. R., Zambryski P. 1993; Phosphorylation of tobacco mosaic virus cell-to-cel) movement protein by a developmentally regulated plant cell wall-associated protein kinase. Genes and Development 7:904–910
    [Google Scholar]
  3. Cooper B., Lapidot M., Heick J. A., Dodds J. A., Beachy R. N. 1995; A defective movement protein of TMV in transgenic plants confers resistance to multiple viruses whereas the functional analog increases susceptibility. Virology 206:307–313
    [Google Scholar]
  4. Deom C. M., Oliver M. J., Beachy R. N. 1987; The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237:389–394
    [Google Scholar]
  5. Deom C. M., Schubert K. R., Wolf S. M., Holt C. A., Lucas W. J., Beachy R. N. 1990; Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proceedings of the National Academy of Sciences, USA 87:3284–3288
    [Google Scholar]
  6. Gafny R., Lapidot M., Berna A., Holt C. A., Deom C. M., Beachy R. N. 1992; Effects of terminal deletion mutations on function of the movement protein of tobacco mosaic virus. Virology 187:499–507
    [Google Scholar]
  7. Hanson M. R., Boeshore M. L., McClean P. E., O’Connell M. A., Nivison H. T. 1988; The isolation of mitochondria and mitochondrial DNA. In Methods for Plant Molecular Biology pp 257–274 Edited by Weissbach A., Weissbach H. San Diego: Academic Press;
    [Google Scholar]
  8. Kaplan I. B., Shintaku M. H., Li Q., Zhang L., Marsh L. E., Palukaitis P. 1995; Complementation of virus movement in transgenic tobacco expressing the cucumber mosaic virus 3a gene. Virology 209:188–199
    [Google Scholar]
  9. Meshi T., Hosokawa D., Kawagishi M., Watanabe Y., Okada Y. 1992; Reinvestigation of intracellular localization of the 30K protein in tobacco protoplasts infected with tobacco mosaic virus RNA. Virology 187:809–813
    [Google Scholar]
  10. Moser O., Gagey M.-J., Godefroy-Colburn T., Stussi-Garaud C., Ellwart-Tschurtz M., Nitschko H., Mundry K.-W. 1988; The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. Journal of General Virology 69:1367–1373
    [Google Scholar]
  11. Nejidat A., Cellier F., Holt C. A., Gafny R., Eggenberger A. L., Beachy R. N. 1991; Transfer of the movement protein gene between two tobamoviruses: influence on local lesion development. Virology 180:318–326
    [Google Scholar]
  12. Nivison H. T., Fish L. E., Jagendorf A. T. 1988; Translation by isolated pea chloroplasts. In Methods for Plant Molecular Biology pp 159–172 Edited by Weissbach A., Weissbach H. San Diego: Academic Press;
    [Google Scholar]
  13. Pascoe M. J., Ingle J. 1978; Distinction between nuclear satellite DNA’s and chloroplast DNA in higher plants. Plant Physiology 62:975–977
    [Google Scholar]
  14. Stern D. B., Newton K. J. 1988; Isolation of plant mitochondrial RNA. In Methods for Plant Molecular Biology pp 309–320 Edited by Weissbach A., Weissbach H. San Diego: Academic Press;
    [Google Scholar]
  15. Tomenius K., Clapham D., Meshi T. 1987; Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus. Virology 160:363–371
    [Google Scholar]
  16. Tsai C.-H., Dreher T. W. 1993; Increased viral yield and symptom severity result from a single amino acid substitution in the turnip yellow mosaic virus movement protein. Molecular Plant-Microbe Interactions 6:268–273
    [Google Scholar]
  17. Turner A., Wells B., Roberts K. 1994; Plasmodesmata of maize root tips: structure and composition. Journal of Cell Science 107:3351–3361
    [Google Scholar]
  18. Vaquero C., Turner A. P., Demangeat G., Sanz A., Serra M. T., Roberts K., Garcia-Luque I. 1994; The 3a protein from cucumber mosaic virus increases the gating capacity of plasmodesmata in transgenic tobacco plants. Journal of General Virology 75:3193–3197
    [Google Scholar]
  19. Watson J. C., Thompson W. F. 1988; Purification and restriction endonuclease analysis of plant nuclear DNA. In Methods for Plant Molecular Biology pp 57–76 Edited by Weissbach A., Weissbach H. San Diego: Academic Press;
    [Google Scholar]
  20. Wolf S., Deom C. M., Beachy R. N., Lucas W. J. 1991; Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein. Plant Cell 3:593–604
    [Google Scholar]
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