1887

Abstract

SUMMARY

The poliovirus replication complex (RC), the site of genomic 36S RNA synthesis, was previously shown to contain subviral particles of 5S protomer and 14S pentamer antigenicity. The present investigation demonstrates that 5S/14S antigenic subviral particles can be cross-linked to viral RNA by UV irradiation of a subcellular fraction containing the poliovirus RC. Each capsid protein of the subviral particles, i.e. VP0, VP1 and VP3, was cross-linked to viral RNA. SDS–PAGE analysis of the cross-linked capsid proteins revealed a bandshift for VP1, whereas VP0 migrated in several bands, which were interpreted to be multimers of VP0 linked by short stretches of RNA. It was found that 36S RNA rather than replicative intermediate RNA was cross-linked to capsid proteins. Our results indicate that encapsidation of poliovirus RNA starts in the RC and is initiated by 14S pentamers.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-1-63
1995-01-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/1/JV0760010063.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-1-63&mimeType=html&fmt=ahah

References

  1. Andino R., Rieckhof G. E., Baltimore D. 1990; A functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA. Cell 63:369–380
    [Google Scholar]
  2. Andino R., Rieckhof G. E., Achacoso P. L., Baltimore D. 1993; Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA. EMBO Journal 12:3587–3598
    [Google Scholar]
  3. Ansardi D. C., Morrow C. D. 1993; Poliovirus capsid proteins derived from PI precursors with glutamine-valine cleavage sites have defects in assembly and RNA encapsidation. Journal of Virology 67:7284–7297
    [Google Scholar]
  4. Baltimore D., Girard M., Darnell J. E. 1966; Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology 29:179–189
    [Google Scholar]
  5. Bienz K., Egger D., Rasser Y., Bossart W. 1980; Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathology. Virology 100:390–399
    [Google Scholar]
  6. Bienz K., Egger D., Pasamontes L. 1987; Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography. Virology 160:220–226
    [Google Scholar]
  7. Bienz K., Egger D., Troxler M., Pasamontes L. 1990; Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region. Journal of Virology 64:1156–1163
    [Google Scholar]
  8. Bienz K., Egger D., Pfister T., Troxler M. 1992; Structural and functional characterization of the poliovirus replication com- plex. Journal of Virology 66:2740–2747
    [Google Scholar]
  9. Caliguiri L. A., Tamm I. 1970; Characterization of poliovirus- specific structures associated with cytoplasmic membranes. Virologv 42:112–122
    [Google Scholar]
  10. Caliguiri L. A., Mosser A. G. 1971; Proteins associated with the poliovirus RNA replication complex. Virology 46:375–386
    [Google Scholar]
  11. Caliguiri L. A., Compans R. W. 1973; The formation of poliovirus particles in association with the RNA replication complexes. Journal of General Virology 21:99–108
    [Google Scholar]
  12. Etchison D., Ehrenfeld E. 1981; Comparison of replication complexes synthesizing poliovirus RNA. Virology 111:33–46
    [Google Scholar]
  13. Filman D. J., Syed R., Chow M., Macadam A. J., Minor P. D., Hogle J. M. 1989; Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8:1567–1579
    [Google Scholar]
  14. Fisher A. J., Johnson J. E. 1993; Ordered duplex RNA controls capsid architecture in an icosahedral animal virus. Nature 361:176–179
    [Google Scholar]
  15. Flanegan J. B., Baltimore D. 1979; Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polymerase. Journal of Virology 29:352–360
    [Google Scholar]
  16. Ghendon Y., Yakobson E., Mikhejeva A. 1972; Study of some stages of poliovirus morphogenesis in MiO cells. Journal of Virology 10:261–266
    [Google Scholar]
  17. Girard M. 1969; In vitro synthesis of poliovirus ribonucleic acid: role of the replicative intermediate. Journal of Virology 3:376–384
    [Google Scholar]
  18. Greenberg J. R. 1980; Proteins crosslinked to messenger RNA by irradiating polyribosomes with ultraviolet light. Nucleic Acids Research 8:5685–5701
    [Google Scholar]
  19. Hellen C. U. T., Wimmer E. 1992; Maturation of poliovirus capsid proteins. Virology 187:391–397
    [Google Scholar]
  20. Hogle J. M., Chow M., Filman D. J. 1985; Three-dimensional structure of poliovirus at 2.9 A resolution. Science 229:1358–1365
    [Google Scholar]
  21. Kattoura M. D., Clapp L. L., Patton J. T. 1992; The rotavirus nonstructural protein, NS35, possesses RNA-binding activity in vitro and in vivo. Virology 191:698–708
    [Google Scholar]
  22. Kuno H., Kihara H. K. 1967; Simple microassay of protein with membrane filter. Nature 215:974–975
    [Google Scholar]
  23. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  24. Larson S. B., Koszelak S., Day J., Greenwood A., Dodds J. A., McPherson A. 1993; Double-helical RNA in satellite tobacco mosaic virus. Nature 361:179–182
    [Google Scholar]
  25. Li J. P., Baltimore D. 1988; Isolation of poliovirus 2C mutants defective in viral RNA synthesis. Journal of Virology 62:4016–4021
    [Google Scholar]
  26. MacDonald R. J., Swift G. H., Przybyla A. E., Chirgwin J. M. 1987; Isolation of RNA using guanidinium salts. Methods in Enzymology 152:219–227
    [Google Scholar]
  27. Marongiu M. E., Pani A., Corrias M. V., Sau M., La Colla P. 1981; Poliovirus morphogenesis. I. Identification of 80S dissociable particles and evidence for the artifactual production of procapsids. Journal of Virology 39:341–347
    [Google Scholar]
  28. Mertens T., Pika U., Eggers J. 1983; Cross antigenicity among enteroviruses as revealed by immunoblot technique. Virology 129:431–442
    [Google Scholar]
  29. Molla A., Paul A. V., Schmid M., Jang S. K., Wimmer E. 1993; Studies on dicistronic poliovirus implicate viral proteinase 2A Pro in RNA replication. Virology 196:739–747
    [Google Scholar]
  30. Nuss D. L., Oppermann H., Koch G. 1975; Selective blockage of initiation of host protein synthesis in RNA-virus-infected cells. Proceedings of the National Academy of SciencesUSA 72:1258–1262
    [Google Scholar]
  31. Oberste M. S., Flanegan J. B. 1988; Measurement of poliovirus RNA polymerase binding to poliovirus and nonviral RNAs using a filter binding assay. Nucleic Acids Research 16:10339–10352
    [Google Scholar]
  32. Pasamontes L., Egger D., Bienz K. 1986; Production of monoclonal and monospecific antibodies against non-capsid proteins of poliovirus. Journal of General Virology 67:2415–2422
    [Google Scholar]
  33. Pfister T., Pasamontes L., Troxler M., Egger D., Bienz K. 1992; Immunocytochemical localization of capsid-related particles in subcellular fractions of poliovirus-infected cells. Virology 188:676–684
    [Google Scholar]
  34. Rombaut B., Vrijsen R., Boeye A. 1990; New evidence for the precursor role of 14S subunits in poliovirus morphogenesis. Virology 111:411–114
    [Google Scholar]
  35. Takeda N., Kuhn R. J., Yang C. -F., Takegami T., Wimmer E. 1986; Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells. Journal of Virology 60:43–53
    [Google Scholar]
  36. Towbin H., Stajehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of SciencesUSA 76:4350–1354
    [Google Scholar]
  37. Wagenmakers A. J. M., Reinders R. J., van Venrooij W. J. 1980; Cross-linking of mRNA to proteins by irradiation of intact cells with ultraviolet light. European Journal of Biochemistry 112:323–330
    [Google Scholar]
  38. Wetz K., Habermehl K. -O. 1982; Specific cross-linking of capsid proteins to virus RNA by ultraviolet irradiation of poliovirus. Journal of General Virology 59:397–101
    [Google Scholar]
  39. Yin F. H. 1977; Involvement of viral procapsid in the RNA synthesis and maturation of poliovirus. Virology 82:299–307
    [Google Scholar]
  40. Zimmern D., Butler P. J. G. 1977; The isolation of tobacco mosaic virus RNA fragments containing the origin for viral assembly. Cell 11:455–462
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-1-63
Loading
/content/journal/jgv/10.1099/0022-1317-76-1-63
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error