1887

Abstract

Two filamentous phages of pv. and pv. were isolated and designated øXv and øXo, respectively. They were similar to other filamentous phages of in (i) shape, (ii) restrictive host specificity, (iii) high stability, (iv) an ssDNA genome, (v) a dsDNA as the replicative form (RF), (vi) propagation without lysis of host cells and (vii) ability to integrate into the host chromosome. These phages showed sequence homology to filamentous phage øLf of pv. . øXv was inactivated by antisera against øXv, øXo and øLf, whereas øXo and øLf were inactivated only by their respective antisera and the anti-øXv serum. Both the single-stranded phage DNAs and the RF DNAs of øXv, øXo and øLf were able to transfect pv. pv. and pv. . Physical maps of øXv and øXo were constructed for the RF DNAs. Genome sizes were estimated, based on mapping data, to be 6.8 kb for øXv and 7.6 kb for øXo, larger than that of the øLf genome (6.0 kb). The difference in genome sizes appeared to result from insertions of large DNA fragments. These fragments and the regions mediating integration were localized in the physical maps.

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/content/journal/jgv/10.1099/0022-1317-75-9-2543
1994-09-01
2019-11-12
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-9-2543
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