1887

Abstract

Two filamentous phages of pv. and pv. were isolated and designated Xv and Xo, respectively. They were similar to other filamentous phages of in (i) shape, (ii) restrictive host specificity, (iii) high stability, (iv) an ssDNA genome, (v) a dsDNA as the replicative form (RF), (vi) propagation without lysis of host cells and (vii) ability to integrate into the host chromosome. These phages showed sequence homology to filamentous phage Lf of pv. Xv was inactivated by antisera against Xv, Xo and Lf, whereas o and Lf were inactivated only by their respective antisera and the anti-Xv serum. Both the single-stranded phage DNAs and the RF DNAs of Xv, Xo and Lf were able to transfect pv. pv. and pv. Physical maps of Xv and Xo were constructed for the RF DNAs. Genome sizes were estimated, based on mapping data, to be 6·8 kb for Xv and 7·6 kb for Xo, larger than that of the ;Lf genome (6·0 kb). The difference in genome sizes appeared to result from insertions of large DNA fragments. These fragments and the regions mediating integration were localized in the physical maps.

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1994-09-01
2022-01-16
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