1887

Abstract

Monoclonal antibodies (MAbs) to equine arteritis virus (EAV) proteins were produced and characterized. The protein specificities of eight MAbs were determined definitively by immunoprecipitation of EAV proteins expressed from vaccinia virus recombinants (WRs). Included were two new WRs produced for this study, expressing the M and the G proteins, respectively. Three MAbs were determined to be N-specific and five MAbs recognized the G protein. One G-specific MAb, 17F5, of the IgA class, efficiently neutralized EAV infectivity. In competitive binding assays (CBAs), the N- specific MAbs defined a single antigenic domain on this protein. Four G-specific MAbs, including MAb 17F5, demonstrated strong reciprocal competition in binding to the G protein but differed in their virus-neutralizing ability. Thus the antigenic domain defined by these MAbs is probably composed of overlapping or closely adjacent epitopes. The fifth G-specific MAb, a nonneutralizing antibody, may define an epitope adjacent to this antigenic domain as reciprocal CBAs demonstrated lower competition.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-9-2439
1994-09-01
2023-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/9/JV0750092439.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-9-2439&mimeType=html&fmt=ahah

References

  1. Balasuriya U. B. R., Rossitto P. V., Demaula C. D., MacLachlan N. J. 1993; A 29K envelope glycoprotein of equine arteritis virus expresses neutralization determinants recognized by murine monoclonal antibodies. Journal of General Virology 74:2525–2529
    [Google Scholar]
  2. Chakrabarti S., Brechling K., Moss B. 1985; Vaccinia virus expression vector: coexpression of β-galactosidase provides visual screening of recombinant virus plaques. Molecular and Cellular Biology 5:3403–3409
    [Google Scholar]
  3. Chirnside E. D., Cook R. F., Lock M. W., Mumford J. A. 1988; Monoclonal antibodies to equine arteritis virus. In Equine Infectious Diseases V: Proceedings of the Fifth International Conference pp. 262–267 Powell D. G. Edited by Lexington: University Press of Kentucky;
    [Google Scholar]
  4. Coutelier J. -P., Van Snick J. 1988; Neutralization and sensitization of lactate dehydrogenase-elevating virus with monoclonal antibodies. Journal of General Virology 69:2097–2100
    [Google Scholar]
  5. Coutelier J. -P., Van Roost E., Lambotte P., Van Snick J. 1986; The murine antibody response to lactate dehydrogenase-elevating virus. Journal of General Virology 67:1099–1108
    [Google Scholar]
  6. Den Boon J. A., Snijder E. J., Chirnside E. D., De Vries A. A. F., Horzinek M. C., Spaan W. J. M. 1991; Equine arteritis virus is not a togavirus but belongs to the coronavirus-like superfamily. Journal of Virology 65:2910–2920
    [Google Scholar]
  7. Deregt D., Babiuk L. A. 1987; Monoclonal antibodies to bovine coronavirus: characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins. Virology 161:410–420
    [Google Scholar]
  8. Deregt D., Sabara M., Babiuk L. A. 1987; Structural proteins of bovine coronavirus and their intracellular processing. Journal of General Virology 68:2863–2877
    [Google Scholar]
  9. Deregt D., Masri S. A., Cho H. J., Bielefeldt Ohmann H. 1991; Bovine viral diarrhea virus: relatedness of pl75 with p80 and p125 and evidence of glycoprotein processing. Canadian Journal of Microbiology 37:815–822
    [Google Scholar]
  10. De Vries A. A. F., Chirnside E. D., Bredenbeek P. J., Gravestein L. A., Horzinek M. C., Spaan W. J. M. 1990; All subgenomic mRNAs of equine arteritis virus contain a common leader sequence. Nucleic Acids Research 18:3241–3247
    [Google Scholar]
  11. De Vries A. A. F., Chirnside E. D., Horzinek M. C., Rottier P. J. M. 1992; Structural proteins of equine arteritis virus. Journal of Virology 66:6294–6303
    [Google Scholar]
  12. Doll E. R., Bryans J. T., McCollum W. H., Crowe M. E. W. 1957a; Isolation of a filterable agent causing arteritis of horses and abortion by mares. Its differentiation from the equine abortion (influenza) virus. Cornell Veterinarian 47:3–41
    [Google Scholar]
  13. Doll E. R., Knappenberger R. E., Bryans J. T. 1957b; An outbreak of abortion caused by equine arteritis virus. Cornell Veterinarian 47:69–75
    [Google Scholar]
  14. Godeny E. K., Chen L., Kumar S. N., Methven S. L., Koonin E. V., Brinton M. A. 1993; Complete genomic sequence and phylogenetic analysis of the lactate dehydrogenase-elevating virus (LDV). Virology 194:585–596
    [Google Scholar]
  15. Harty J. T., Plagemann P. G. W. 1988; Formalin inactivation of the lactate dehydrogenase-elevating virus reveals a major neutralizing epitope not recognized during natural infection. Journal of Virology 62:3210–3216
    [Google Scholar]
  16. Harty J. T., Chan S. P. K., Plagemann P. G. W. 1987; Characteristics of monoclonal antibodies to the lactate dehydrogenase-elevating virus. Intervirology 27:53–60
    [Google Scholar]
  17. Huntington P. J., Ellis P. M., Forman A. J., Timoney P. J. 1990; Equine arteritis virus. Australian Veterinary Journal 67:429–431
    [Google Scholar]
  18. Hyllseth B. 1973; Structural proteins of equine arteritis virus. Archiv für die gesamte Virusforschung 40:177–188
    [Google Scholar]
  19. Jameson B. A., Wolf H. 1988; The antigenic index: a novel algorithm for predicting antigenic determinants. Computer Applications in the Biosciences 4:181–186
    [Google Scholar]
  20. Kimura-Kuroda J., Yasui K. 1983; Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus, using monoclonal antibodies. Journal of Virology 45:124–132
    [Google Scholar]
  21. Köhler G., Milstein C. 1976; Derivation of specific antibody- producing tissue culture and tumor lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  22. Krijnse Locker J., Rose J. K., Horzinek M. C., Rottier P. J. M. 1992; Membrane assembly of the triple-spanning coronavirus M protein. Journal of Biological Chemistry 267:21911–21918
    [Google Scholar]
  23. Kunkel T. A., Bebenek K., McClary J. 1991; Efficient site- directed mutagenesis using uracil-containing DNA. Methods in Enzymology 204:125–139
    [Google Scholar]
  24. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  25. Lussenhop N. O., Goertz R., Wabuke-Bunoti M., Gehrz R., Kari B. 1988; Epitope analysis of human cytomegalovirus glycoprotein complexes using murine monoclonal antibodies. Virology 164:362–372
    [Google Scholar]
  26. Meulenberg J. J. M., Hulst M. M., De Meijer E. J., Moonen P. L. J. M., Den Besten A., De Kluyver E. P., Wensvoort G., Moormann R. J. M. 1993; Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192:62–72
    [Google Scholar]
  27. Porterfield J. S., Casals J., Chumakov M. P., Gaidamovich S. Y., Hannoun C., Holmes I. H., Horzinek M. C., Mussgay M., Okerblom N., Russell P. K., Trent D. W. 1978; Togaviridae. Intervirology 9:129–148
    [Google Scholar]
  28. Rohrmann G., Yuen L., Moss B. 1986; Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence. Cell 46:1029–1035
    [Google Scholar]
  29. Timoney P. J., McCollum W. H., Roberts A. W., Murphy T. W. 1986; Demonstration of the carrier state in naturally acquired equine arteritis virus infection in the stallion. Research in Veterinary Science 41:279–280
    [Google Scholar]
  30. Van Berlo M. F., Zeegers J. J. W., Horzinek M. C., Van Der Zeijst B. A. M. 1983; Antigenic comparison of equine arteritis virus (EAV) and lactic dehydrogenase virus (LDV); binding of staphylococcal protein A to the nucleocapsid protein of EAV. Zentralblatt fur Veterinarmedizin Reihe B 30:297–304
    [Google Scholar]
  31. Van Berlo M. F., Rottier P. J. M., Spaan W. J. M., Horzinek M. C. 1986; Equine arteritis virus-induced polypeptide synthesis. Journal of General Virology 67:1543–1549
    [Google Scholar]
  32. Zeegers J. J. W., Van Der Zeijst B. A. M., Horzinek M. C. 1976; The structural proteins of equine arteritis virus. Virology 73:200–205
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-9-2439
Loading
/content/journal/jgv/10.1099/0022-1317-75-9-2439
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error