1887

Abstract

In order to study the function of human cytomegalovirus (HCMV) immediate early gene 2 () (UL122) gene products made at late times during infection, cDNA clones were isolated from an expression library made with 74 h post-infection mRNA. Based on screening of the library, 1 % of transcripts in infected cells at this time were region-specific, and transcripts encoding IE2, a 40K late gene product, were more abundant than those encoding IE2 , an a gene product made throughout infection. As expected, the cDNA capable of directing the expression of IE2 was derived from a contiguous genomic region within exon 5 of the region. The cDNA clones encoding IE2 and IE2 were compared for their ability to trans-activate viral and cellular promoters and to repress expression from the promoter via the cis-repression signal. Unexpectedly, IE2 trans-activated a variety of test promoters when cotransfected with the major a gene product, IE1. Promoters derived from the cellular -actin gene, the simian virus 40 early region and the human immunodeficiency virus were all responsive to IE2 plus IE1, although several promoters derived from the HCMV genome were unresponsive. Thus, this abundant late product from the region may play a role in trans-activation in addition to its role as a repressor of gene expression.

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1994-09-01
2021-10-26
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