1887

Abstract

Rotavirus strain A253, isolated from the faeces of a diarrhoeic piglet in Venezuela, was classified as serotype G11 by cross-neutralization studies and by comparison of the deduced amino acid sequence of the VP7 surface protein. The epitopes involved in neutralization of the two G11 porcine rotavirus strains A253 and YM were analysed using neutralization-resistant mutants selected with seven neutralizing monoclonal antibodies (MAbs), monotype-specific (M-) MAbs and serotype-specific (S-) MAbs, produced against VP7 of strain A253. Crossneutralization tests and sequence analysis of the escape mutants selected from strains A253 and YM indicated the presence of two antigenic sites, one common to both M-MAbs and S-MAbs in region A (positions 87, 91 and 96) and the other defined by one S-MAb in region C (position 223). All A253 variants selected with M-MAbs and two S-MAbs, although having different amino acid substitutions, had a change at amino acid position 87, whereas YM variants involved residues 91 and 96, part of the same antigenic site. Compared to strain A253, the YM strain presents an amino acid substitution at position 87 and was not recognized by M-MAbs. These results suggest that in the VP7 of G11 serotype specificity, the amino acid at position 87 is an important component of a neutralization site associated with region A and the intraserotypic variation between strains A253 and YM may account for the selection of mutations at different positions by a single MAb.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-8-1867
1994-08-01
2022-05-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/8/JV0750081867.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-8-1867&mimeType=html&fmt=ahah

References

  1. Air G. M., Laver W. G., Webster R. G. 1990; Mechanism of antigenic variation in an individual epitope on influenza virus N9 neuraminidase. Journal of Virology 64:5797–5803
    [Google Scholar]
  2. Bellinzoni R. C., Mattion N. M., Matson D. O., Blackhall J., La Torre J. L., Scodeller E. A., Urasawa S., Taniguchi K., Estes M. K. 1990; Porcine rotaviruses antigenicaliy related to human rotavirus serotypes 1 and 2. Journal of Clinical Microbiology 28:633–636
    [Google Scholar]
  3. Bohl E. H., Theil K. W., Saif L. J. 1984; Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. Journal of Clinical Microbiology 19:105–111
    [Google Scholar]
  4. Browning G. F., Chalmers R. M., Fitzgerald T. A., Snodgrass D. R. 1991a; Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. Journal of General Virology 72:1059–1064
    [Google Scholar]
  5. Browning G. F., Fitzgerald T. A., Chalmers R. M., Snodgrass D. R. 1991b; A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. Journal of Clinical Microbiology 29:2043–2046
    [Google Scholar]
  6. Coulson B. S., Kirkwood C. 1991; Relation of VP7 amino acid sequence to monoclonal antibody neutralization of rotavirus and rotavirus monotype. Journal of Virology 65:5968–5974
    [Google Scholar]
  7. Dayhoff M. O., Eck R. V. 1968 Atlas of Protein Sequence and Structure pp. 43–45 Silver Spring: National Biomedical Research Foundation;
    [Google Scholar]
  8. Dunn S. T., Ward R. L., McNeal M. M., Cross T. L., Greenberg H. B. 1993; Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions. Virology 197:397–404
    [Google Scholar]
  9. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proceedings of the National Academy of Sciences, U.S.A 83:3465–3468
    [Google Scholar]
  10. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  11. Gorziglia M., Aguirre Y., Hoshino Y., Esparza J., Blumentals I., Askaa J., Thompson M., Glass R. I., Kapikian A. Z., Chanock R. M. 1986; VP7 serotype-specific glycoprotein of OSU porcine rotavirus: coding assignment and gene sequence. Journal of General Virology 67:2445–2454
    [Google Scholar]
  12. Gorziglia M., Larralde G., Ward R. 1990; Neutralization epitopes on rotavirus SA11 4fM outer capsid proteins. Journal of Virology 64:4534–4539
    [Google Scholar]
  13. Green K. Y., Midthun K, Gurziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. 1987; Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology 161:153–159
    [Google Scholar]
  14. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. 1985; Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences, U.S.A 82:8701–8704
    [Google Scholar]
  15. Hoshino Y., Saif L. J., Sereno M. M., Chanock R. M., Kapikian A. Z. 1988; Infection immunity of piglets to either VP3 or VP7 outer capsid protein confers resistance to challenge with a virulent rotavirus bearing the corresponding antigen. Journal of Virology 62:744–748
    [Google Scholar]
  16. Huang J., Nagesha H. S., Dyall-Smith M. L., Holmes I. H. 1989; Comparative sequence analysis of VP7 genes from five Australian porcine rotaviruses. Archives of Virology 109:173–183
    [Google Scholar]
  17. Kang S. -Y., Saif L. J., Miller K. 1989; Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses. Journal of Clinical Microbiology 27:2744–2750
    [Google Scholar]
  18. Kirkwood C., Masendycz P. L., Coulson B. S. 1993; Characteristics and location of cross-reactive and serotype-specific neutralization sites on VP7 of human G type 9 rotaviruses. Virology 196:79–88
    [Google Scholar]
  19. Kobayashi N., Taniguchi K., Urasawa S. 1991; Analysis of the newly identified neutralization epitopes on VP7 of human rotavirus serotype 1. Journal of General Virology 72:117–124
    [Google Scholar]
  20. Kool D. A., Holmes I. H. 1993; The avian rotavirus Ty-1 VP7 nucleotide and deduced amino acid sequences differ significantly from those of Ch-2 rotavirus. Archives of Virology 129:227–234
    [Google Scholar]
  21. Liprandi F., Lopez G., Rodriguez I., Hidalgo M., Ludert J. E., Mattion N. 1990; Monoclonal antibodies to the VP6 of porcine subgroup I rotaviruses reactive with subgroup I and non-subgroup I non-subgroup II strains. Journal of General Virology 71:1395–1398
    [Google Scholar]
  22. Liprandi F., Rodriguez I., Piña C., Larralde G., Gorziglia M. 1991; VP4 monotype specificities among porcine rotaviruses strains of the same VP4 serotype. Journal of Virology 65:1658–1661
    [Google Scholar]
  23. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. 1988; Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology 165:511–517
    [Google Scholar]
  24. Matsui S. M., Mackow E. R., Greenberg H. B. 1989; Molecular determinants of rotavirus neutralization and protection. Advances in Virus Research 36:181–214
    [Google Scholar]
  25. Nagesha H. S., Holmes I. H. 1988; New porcine rotavirus serotype antigenicaliy related to human rotavirus serotype 3. Journal of Virology 175:171–174
    [Google Scholar]
  26. Nishikawa K., Hoshino Y., Taniguchi K., Green K. Y., Greenberg H. B., Kapikian A. Z., Chanock R. M., Gorziglia M. 1989; Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology 171:503–515
    [Google Scholar]
  27. Nishikawa K., Hoshino Y., Gorziglia M. 1991; Sequence of the VP7 gene of chicken rotavirus Ch2 of serotype 7 rotavirus. Virology 185:853–856
    [Google Scholar]
  28. Offit P. A., Clark H. F., Blavat G., Greenberg H. B. 1986; Reassortant rotaviruses containing structural proteins VP3 and VP7 from different parents induce antibodies protective against each parental serotype. Journal of Virology 60:491–196
    [Google Scholar]
  29. Paul P. S., Lyoo Y. S., Andrews J. J., Hill H. T. 1988; Isolation of two new serotypes of porcine rotavirus from pigs with diarrhea. Archives of Virology 100:139–143
    [Google Scholar]
  30. Raj P., Matson D. O., Coulson B. S., Bishop R. F., Taniguchi K., Urasawa S., Greenberg H. B., Estes M. K. 1992; Comparisons of rotavirus VP7-typing monoclonal antibodies by competition binding assay. Journal of Clinical Microbiology 30:704–711
    [Google Scholar]
  31. Ruiz A. M., López I. V., López S., Espejo R. T., Arias C. F. 1988; Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. Journal of Virology 62:4331–4336
    [Google Scholar]
  32. Shaw R. D., Stoner-Ma D. L., Estes M. K., Greenberg H. B. 1985; Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3. Journal of Clinical Microbiology 22:286–291
    [Google Scholar]
  33. Snodgrass D., Fitzgerald I., Campbell I., Scott F. M. M., Browning G. F., Miller D. L., Herring A. J., Greenberg H. B. 1990; Rotavirus serotypes 6 and 10 predominate in cattle. Journal of Clinical Microbiology 28:504–507
    [Google Scholar]
  34. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. 1988; Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. Journal of Virology 62:1870–1874
    [Google Scholar]
  35. Taniguchi K., Urasawa T., Kobayashi N., Gorziglia M., Urasawa S. 1990; Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup 1 specificity and long RNA pattern: implication for new G serotype specificity. Journal of Virology 64:5640–5644
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-8-1867
Loading
/content/journal/jgv/10.1099/0022-1317-75-8-1867
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error