1887

Abstract

Beet necrotic yellow vein virus (BNYW)-infected sugarbeets were obtained from many parts of Europe and also from some sites in Asia and the U.S.A. Reverse transcription (RT)–PCR products of more than 1 kbp were obtained for four different regions of the viral genome which may be particularly important with respect to the pathogenic properties of the virus, i.e. for the coat protein and the 42K protein-encoding regions on RNA 2 and for major parts of RNAs 3 and 4. Restriction fragment length polymorphism (RFLP) patterns obtained with these PCR products revealed the existence of two major strain groups of BNYW, named type A and type B. The A type was detected in Greece, the former Yugoslavia, Slovakia, parts of Austria, Italy, Spain, parts of France, Belgium, The Netherlands and England as well as in Asia (Turkey, Kazachstan, China and Japan) and the U.S.A. The B type occurs in Germany and parts of France. Mixed infections were detected at the borderline regions between areas of the A and B types. Comparisons of published and newly determined nucleotide sequences of the respective parts of the BNYW genome indicate that the percentage of nucleotide differences between the A and the B type is approximately 3 % for the respective regions of RNAs 2 and 3 and approximately 1·5% for RNA 4. Nucleotide sequences appear to be remarkably stable within each of the two strain groups. The majority of the nucleotide differences between the A and B types occur in the third triplet position. The amino acid changes in the coat protein area are outside the four previously determined antigenic regions that are accessible on the surface of the virus particles and are involved in the formation of continuous and presumably also discontinuous epitopes. This may explain why serological differences between the two strain groups have not been found.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-8-1835
1994-08-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/8/JV0750081835.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-8-1835&mimeType=html&fmt=ahah

References

  1. Bos L. 1983; Plant virus ecology; the role of man, and the involvement of governments and international organizations. In Plant Virus Epidemiology pp. 9–23 Plumb R. T., Thresh J. M. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  2. Bouzoubaa S., Guilley H., Jonard G., Richards K., Putz C. 1985; Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and Gl. Journal of General Virology 66:1553–1564
    [Google Scholar]
  3. Bouzoubaa S., Ziegler V., Beck D., Guilley H., Richards K., Jonard G. 1986; Nucleotide sequence of beet necrotic yellow vein virus RNA-2. Journal of General Virology 67:1689–1700
    [Google Scholar]
  4. Briddon R. W., Watts J., Markham P. G., Stanley J. 1989; The coat protein of beet curly top virus is essential for infectivity. Virology 172:628–633
    [Google Scholar]
  5. Buddenhagen I.W. 1983; Crop improvement in relation to virus diseases and their epidemiology. In Plant Virus Epidemiology pp. 25–37 Plumb R. T., Thresh J. M. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  6. Burgermeister W., Koenig R., Weich H., Sebald W., Lesemann D. -E. 1986; Diversity of the RNAs in thirteen isolates of beet necrotic yellow vein virus in Chenopodium quinoa detected by means of cloned cDNAs. Phytopathologische Zeitschrift 115:229–242
    [Google Scholar]
  7. Canova A. 1959; Appunti di patologia della barbabietola. Informatore Fitopatologico 9:390–396
    [Google Scholar]
  8. Commandeur U., Jarausch W., Li Y., Koenig R., Burgermeister W. 1991; cDNAs of beet necrotic yellow vein virus RNAs 3 and 4 are rendered biologically active in a plasmid containing the cauliflower mosaic virus 35S promoter. Virology 185:493–495
    [Google Scholar]
  9. Commandeur U., Koenig R., Lesemann D. -E., Torrance L., Burgermeister W., Liu Y., Schots A., Alric M., Grassi G. 1992; Epitope mapping on fragments of beet necrotic yellow vein virus coat protein. Journal of General Virology 73:695–700
    [Google Scholar]
  10. Commandeur U., Koenig R., Manteuffel R., Torrance L., Lüddecke P., Frank R. 1994; Location, size and complexity of epitopes on the coat protein of beet necrotic yellow vein virus studied by means of synthetic overlapping peptides. Virology 198:282–287
    [Google Scholar]
  11. Dawson W. O., Bubrik P., Rantham G. L. 1988; Modifications of the tobacco mosaic virus coat protein gene affecting replication, movement, and symptomatology. Phytopathology 78:783–789
    [Google Scholar]
  12. Ehlers U., Commandeur U., Frank R., Landsmann J., Koenig R., Burgermeister W. 1991; Cloning of the coat protein gene from beet necrotic yellow vein virus and its expression in sugarbeet hairy roots. Theoretical and Applied Genetics 81:777–782
    [Google Scholar]
  13. Fuchs R., Blakesley R. 1983; Guide to the use of type II restriction endonucleases. Methods in Enzymology 100:3–38
    [Google Scholar]
  14. Gilmer D., Bouzoubaa S., Hehn A., Guilley H., Richards K., Jonard G. 1992; Efficient cell-to-cell movement of beet necrotic yellow vein virus requires 3′ proximal genes located on RNA 2. Virology 189:40–47
    [Google Scholar]
  15. Hamdorf G., Lesemann D. -E. 1979; Untersuchungen fiber das Vorkommen des beet necrotic yellow vein virus (BNYVV) in Hessen und Rheinland-Pfalz. Nachrichtenblatt filr den Deutschen Pflanzenschutzdienst 31:149–153
    [Google Scholar]
  16. Hamdorf G., Lesemann D. -E., Weidemann H. L. 1977; Untersuchungen fiber die Rizomania-Krankheit an Zuckerrfiben in der Bundesrepublik Deutschland. Phytopathologische Zeitschrift 90:97–103
    [Google Scholar]
  17. Heijbrook W. 1984; Distribution of BNYVV in the Netherlands and initiated research programme. Sugar Beet Rhizomania. Proceedings of the First International Conference of the Virologists, Colmar95–96
    [Google Scholar]
  18. Henry C. M., Barker I., Morris J., Hugo S. A. 1993; Detection of beet necrotic yellow vein virus (BNYVV) using polymerase chain reaction (PCR). IXth International Congress of Virology, Glasgow,8-13 August 1993 Abstract P70-17
    [Google Scholar]
  19. Jupin I., Guilley H., Richards K., Jonard G. 1992; Two proteins encoded by beet necrotic yellow vein virus RNA 3 influence symptom phenoptype on leaves. EMBO Journal 11:479–488
    [Google Scholar]
  20. Kaufmann A., Koenig R., Lesemann D. -E. 1992; Tissue print-immunoblotting reveals an uneven distribution of beet necrotic yellow vein and beet soil-borne viruses in sugarbeets. Archives of Virology 126:329–335
    [Google Scholar]
  21. Koenig R., Burgermeister W., Weich H., Sebald W., Kothe C. 1986; Uniform RNA patterns of beet necrotic yellow vein virus in sugarbeet roots, but not in leaves from several plant species. Journal of General Virology 67:2043–2046
    [Google Scholar]
  22. Koenig R., Jarausch W., Li Y., Commandeur U., Burgermeister W., Gehrke M., Lüddecke P. 1991; Effect of recombinant beet necrotic yellow vein virus with different RNA compositions on mechanically inoculated sugarbeets. Journal of General Virology 72:2243–2246
    [Google Scholar]
  23. Kuszala M., Ziegler R., Bouzoubaa S., Richards K., Putz C., Guilley H., Jonard G. 1986; Beet necrotic yellow vein virus: different isolates are serologically similar but differ in RNA composition. Annals of Applied Biology 109:155–162
    [Google Scholar]
  24. Lewellen R. T. 1992; Use of plant introductions to improve populations and hybrids of sugarbeet. Use of Plant Introductions in Cultivar Development, Part 2, CSSA Special Publication20 20:117–136
    [Google Scholar]
  25. Meulewater F., Soetaert P., Van Emmelo J. 1989; Structural analysis of the coat protein gene in different BNYVV isolates. Mededeling van de Faculteit Landbouwwetenschappen Rijkuniversiteit Gent 54/2b:465–168
    [Google Scholar]
  26. Putz C., Vuittenez A. 1974; Observation des particules virales chez des betteraves presentant, en Alsace, des symptomes de ‘Rhizomanie’. Annales de Phytopathologie 6:129–138
    [Google Scholar]
  27. Richards K., Jonard G., Guilley H., Ziegler V., Putz C. 1985; In vitro translation of beet necrotic yellow vein virus RNA and studies of sequence homology among the RNA species using cloned cDNA probes. Journal of General Virology 66:345–350
    [Google Scholar]
  28. Richards K., Tamada T. 1992; Mapping functions on the multipartite genome of beet necrotic yellow vein virus. Annual Review of Phytopathology 30:291–313
    [Google Scholar]
  29. Saito T., Yamanaka K., Okada Y. 1990; Long-distance movement and viral assembly of tobacco mosaic virus mutants. Virology 176:329–336
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-termination inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  31. Schlang J., Lesemann D. -E. 1983; Auftreten der Virosen Wurzelbartigkeit (Rizomania) an Zuckerrfiben im Rheinland. Jahresbericht der Biologischen Bundesanstalt für Land- und Forstwirtschaft H 68:
    [Google Scholar]
  32. Skotnicki M., Mackenzie A. M., Ding S. W., Mo J. Q., Gibbs A. J. 1993; RNA hybrid mismatch polymorphisms in Australian populations of turnip yellow mosaic tymovirus. Archives of Virology 132:83–99
    [Google Scholar]
  33. Tamada T., Abe H. 1989; Evidence that beet necrotic yellow vein virus RNA-4 is essential for efficient transmission by the fungus Polymyxa betae . Journal of General Virology 70:3391–3398
    [Google Scholar]
  34. Tamada T., Shirako Y., Abe H., Saito M., Kiguchi T., Harada T. 1989; Production and pathogenicity of isolates of beet necrotic yellow vein virus with different numbers of RNA components. Journal of General Virology 70:3399–3409
    [Google Scholar]
  35. Thresh J. M. 1991; The ecology of tropical plant viruses. Plant Pathology 40:324–339
    [Google Scholar]
  36. Torrance L., Pead M. T., Buxton G. 1988; Production and some characteristics of monoclonal antibodies against beet necrotic yellow vein virus. Annals of Applied Biology 113:519–530
    [Google Scholar]
  37. Verwoerd T. C., Dekker B. M. M., Hoekema A. 1989; A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17:2362
    [Google Scholar]
  38. Yao H., Liu Y., Zhunan C., Yu Z. 1993; The cloning and sequencing of coat protein gene from beet necrotic yellow vein virus. Chinese Journal of Biotechnology 2:147–151
    [Google Scholar]
  39. Ziegler V., Richards K., Guilley H., Jonard G., Putz C. 1985; Cell-free translation of beet necrotic yellow vein virus: readthrough of the coat protein cistron. Journal of General Virology 66:2079–2087
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-8-1835
Loading
/content/journal/jgv/10.1099/0022-1317-75-8-1835
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error