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The tropism of human immunodeficiency virus (HIV) for macrophages (mรธ)is a well recognized phenomenon, but the range and distribution of m๐-tropic phenotypes have not been defined by quantitative means. This study uses a PCR-based infectivity assay to derive an index of m๐ tropism for several common strains of HIV. The results show that m๐ tropism varies over about six orders of magnitude and that the most m๐-tropic strains have a higher infectivity for m๐ than for peripheral blood lymphocytes. Strains were distributed throughout this range, suggesting that m๐ tropism is a continuously variable phenotypic property. Although the degree of tropism was strongly influenced by the mode of isolation and propagation of virus strains, there was no evidence for the existence of distinct m๐-tropic or non-m๐-tropic phenotypes. Finally, the tropism of two selected strains was found to be determined by an early step in replication, probably virus entry.
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