1887

Abstract

Twenty-six polypeptides in murine herpesvirus isolate 72 (MHV-72) were identified and the synthesis and accumulation of 21 virus-specific polypeptides in Vero cells during the course of productive infection with MHV-72 were examined. Five of the infected cell polypeptides accumulated within the nuclei and nine accumulated within the cytoplasm of MHV-72-infected cells. Seven polypeptides were identified within the nuclei and cytoplasm in equivalent amounts. The major capsid protein was shown to have an of 161K. Thirteen virus-specific polypeptides were solubilized with Nonidet P-40 or radioimmunoprecipitation assay buffer and immunoprecipitated with rabbit and mouse immune sera. Analysis of the polypeptides of MHV-72 indicate a closer resemblance between MHV-72 and the gammaherpesviruses herpesvirus saimiri and Epstein- Barr virus than with the alphaherpesvirus herpes simplex virus type 1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-6-1259
1994-06-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/6/JV0750061259.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-6-1259&mimeType=html&fmt=ahah

References

  1. Baer R., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C., Tuffnell P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature; London: 310207–211
    [Google Scholar]
  2. Blair E. D., Honess R. W. 1983; DNA-binding proteins specified by herpesvirus saimiri. Journal of General Virology 64:2697–2715
    [Google Scholar]
  3. Blaskovic D., Stancekova M., Svobodova J., Kresakova J. 1980; Isolation of five strains of herpesvirus from two species of free-living small rodents. Acta virologica 24:468
    [Google Scholar]
  4. Blaskovic D., Stancekova M., Rajcani J. 1984; Experimental pathogenesis of murine herpesvirus in newborn mice. Acta virologica 28:225–231
    [Google Scholar]
  5. Bublot M., Lomonte P., Lequarre A. S., Albrecht J. C., Nicholas J., Fleckenstein B., Pastoret P. P., Thiry E. 1992; Genetic relationships between bovine herpesvirus 4 and the gammaherpesviruses Epstein-Barr virus and herpesvirus saimiri. Virology 190:654–665
    [Google Scholar]
  6. Cameron K. R., Stamminger T., Craxton M., Bodemer W., Honess R. W., Fleckenstein B. 1987; The 160000 Mr virion protein encoded at the right end of the herpesvirus saimiri genome is homologous to the 140000 Mr membrane antigen encoded at the left end of the Epstein-Barr virus genome. Journal of Virology 61:2063–2070
    [Google Scholar]
  7. Ciampor F., Stancekova M., Blaskovic D. 1981; Electron microscopy of rabbit embryo fibroblasts infected with herpesvirus isolates from Clethrionomys glareolusand Apodemus flavicollis . Acta virologica 25:101–107
    [Google Scholar]
  8. Dubuisson J., Boulanger D., Bublot M., Thiry E., Pastoret P.-P. 1989; Proteins specified by bovine herpesvirus type 4: structural proteins of the virion and identification of two major glycoproteins by using monoclonal antibodies. Journal of General Virology 70:1743–1753
    [Google Scholar]
  9. Efstathiou S., Ho Y. M., Minson A. C. 1990a; Cloning and molecular characterization of the murine herpesvirus 68 genome. Journal of General Virology 71:1355–1364
    [Google Scholar]
  10. Efstathiou S., Ho Y. M., Hall S., Styles C. J., Scott S. D., Gompels U. A. 1990b; Murine herpesvirus 68 is genetically related to the gammaherpesviruses Epstein-Barr virus and herpesvirus saimiri. Journal of General Virology 71:1365–1372
    [Google Scholar]
  11. Irmiere A., Gibson W. 1985; Isolation of human cytomegalovirus intranuclear capsids, characterisation of their protein constituents, and demonstration that the B-capsid assembly protein is also abundant in noninfectious enveloped particles. Journal of Virology 56:277–283
    [Google Scholar]
  12. Kawanishi M., Sugawara K., Ito Y. 1981; Epstein-Barr virus- induced early polypeptides in Raji and NC37 cells activated by diterpene ester TPA in combination with n-butyrate. Virology 115:406–409
    [Google Scholar]
  13. Kozuch O., Reichel M., Lesso J., Remenova A., Labuda M., Lysy J. 1993; New isolation of murine herpesviruses from small mammals in southwestern Slovakia. Acta virologica 37:101–105
    [Google Scholar]
  14. Matis J., Rajcani J. 1980; Preparation of immune serum to immediate early and early polypeptides specified by herpes simplex type 1. Acta virologica 24:105–113
    [Google Scholar]
  15. Mueller-Lantzsch N., Yamamoto N., Zur Hausen H. 1979; Analysis of early and late Epstein-Barr virus associated polypeptides by immunoprecipitation. Virology 97:378–387
    [Google Scholar]
  16. Rajcani J., Blaskovic D., Svobodova J., Ciampor F., Huckova D., Stanekova D. 1985; Pathogenesis of acute and persistent murine herpesvirus infection in mice. Acta virologica 29:51–60
    [Google Scholar]
  17. Raucina J., Matis J., Lesso J. 1984; Defective glycoprotein C of the syncytial strain of herpes simplex virus type 1. Acta virologica 28:457–463
    [Google Scholar]
  18. Reichel M., Matis J., Lesso J., Stancekova M. 1991; Polypeptides synthesized in rabbit cells infected with murine herpesvirus (MHV): a comparison of proteins specified by various MHV strains. Acta virologica 35:268–275
    [Google Scholar]
  19. Seal B. S., Kliefort R. B., Welch W. H., Heuschele W. P. 1989; Alcelaphine herpesvirus 1 and 2 SDS-PAGE analysis of virion polypeptides, restriction endonuclease analysis of genomic DNA and virus replication restriction in different cell types. Archives of Virology 106:301–320
    [Google Scholar]
  20. Sunil-Chandra N. P., Efstathiou S., Arno J., Nash A. A. 1992a; Virological and pathological features of mice infected with murine gammaherpesvirus 68. Journal of General Virology 73:2347–2356
    [Google Scholar]
  21. Sunil-Chandra N. P., Efstathiou S., Nash A. A. 1992b; Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo . Journal of General Virology 73:3275–3279
    [Google Scholar]
  22. Svobodova J., Blaskovic D., Mistrikova J. 1982a; Growth characteristics of herpesviruses isolated from free-living small rodents. Acta virologica 26:256–263
    [Google Scholar]
  23. Svobodova J., Stancekova M., Blaskovic D., Mistrikova J., Lesso J., Russ G., Masarova P. 1982b; Antigenic relatedness of alphaherpesviruses isolated from free-living rodents. Acta virologica 26:438–443
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-6-1259
Loading
/content/journal/jgv/10.1099/0022-1317-75-6-1259
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error