1887

Abstract

Mouse infection with lactate dehydrogenase-elevating virus (LDV) leads to lifelong viraemia, despite the production of neutralizing antiviral antibodies. To test whether viral persistence correlated with the development of resistance to these antibodies, we compared the neutralization of viral particles derived from acutely and chronically infected animals, using polyclonal and monoclonal anti-LDV antibodies. Whereas virus isolated during acute infection was efficiently neutralized, titres of LDV from chronically infected mice were only slightly reduced by antiviral antibodies. In addition, LDV from animals acutely infected with such poorly neutralizable virus from chronically infected mice was resistant to anti-LDV antibodies like their parental viral particles. These results suggest that LDV variants capable of escaping neutralization by antiviral antibodies can emerge in chronically infected animals.

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1994-05-01
2021-10-25
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References

  1. Brinton M. A., Gavin E. I., Fernandez A. V. 1986; Genotypic variation among six isolates of lactate dehydrogenase-elevating virus. Journal of General Virology 67:2673–2684
    [Google Scholar]
  2. Cafruny W. A., Plagemann P. G. W. 1982; Immune response to lactate dehydrogenase-elevating virus: serologically specific rabbit neutralizing antibody to the virus. Infection and Immunity 37:1007–1012
    [Google Scholar]
  3. Cafruny W. A., Chan S. P. K., Harty J. T., Yousefi S., Kowalchyk K., McDonald D., Foreman B., Budweg G., Plagemann P. G. W. 1986; Antibody response of mice to lactate dehydrogenase-elevating virus during infection and immunization with inactivated virus. Virus Research 5:357–375
    [Google Scholar]
  4. Clements J. E., Pedersen F. S., Narayan O., Haseltine W. A. 1980; Genomic changes associated with antigenic variation of visna virus during persistent infection. Proceedings of the National Academy of Sciences U.S.A 77:4454–4458
    [Google Scholar]
  5. Coutelier J. -P., Van Snick J. 1988; Neutralization and sensitization of lactate dehydrogenase-elevating virus with monoclonal antibodies. Journal of General Virology 69:2097–2100
    [Google Scholar]
  6. Coutelier J. -P., Van Roost E., Lambotte P., Van Snick J. 1986; The murine antibody response to lactate dehydrogenase-elevating virus. Journal of General Virology 67:1099–1108
    [Google Scholar]
  7. De Vries A. A. F., Chirnside E. D., Horzinek M. C., Rottier P. J. M. 1992; Structural proteins of equine arteritis virus. Journal of Virology 66:6294–6303
    [Google Scholar]
  8. Godeny E. K., Chen L., Kumar S. N., Metheven S. L., Koonin E. V., Brinton M. A. 1993; Complete genomic sequence and phylogenetic analysis of the lactate dehydrogenase-elevating virus (LDV). Virology 194:585–596
    [Google Scholar]
  9. Hahn B. H., Shaw G. M., Taylor M. E., Redfield R. R., Markham P. D., Salahuddin S. Z., Wong-Staal F., Gallo R. C., Parks E. S., Parks W. P. 1986; Genetic variation in HTLV-III/LAV over time in patients with AIDS or at risk for AIDS. Science 232:1548–1553
    [Google Scholar]
  10. Harty J. T., Plagemann P. G. W. 1988; Formalin inactivation of the lactate dehydrogenase-elevating virus reveals a major neutralizing epitope not recognized during natural infection. Journal of Virology 62:3210–3216
    [Google Scholar]
  11. Harty J. T., Plagemann P. G. W. 1990; Monoclonal antibody protection from age-dependent poliomyelitis: implications regarding the pathogenesis of lactate dehydrogenase-elevating virus. Journal of Virology 64:6257–6262
    [Google Scholar]
  12. Kuo L., Chen Z., Rowland R. R. R., Faaberg K. S., Plagemann P. G. W. 1992; Lactate dehydrogenase-elevating virus (LDV): subgenomic mRNAs, mRNA leader and comparison of 3′-terminal sequences of two LDV isolates. Virus Research 23:55–72
    [Google Scholar]
  13. Meulenberg J. J. M., Hulst M. M., De Meier E. J., Moonen P. L. J. M., Den Besten A., De Kluyver E. P., Wensvoort G., Moormann R. J. M. 1993; Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192:62–72
    [Google Scholar]
  14. Notkins A. L., Mahar S., Scheele C., Goffman J. 1966; Infectious virus-antibody complex in the blood of chronically infected mice. Journal of Experimental Medicine 124:81–97
    [Google Scholar]
  15. Notkins A. L., Mage M., Ashe W. K., Mahar S. 1968; Neutralization of sensitized lactic dehydrogenase virus by anti-γ-globulin. Journal of Immunology 100:314–320
    [Google Scholar]
  16. Onyekaba C. O., Harty J. T., Even C., Hu B. -G., Plagemann P. G. W. 1989; Persistent infection of mice by lactate dehydrogenase-elevating virus: effects of immunosuppression on virus replication and antiviral immune responses. Virus Research 14:297–316
    [Google Scholar]
  17. Payne S., Parekh B., Montelako R. C., Issel C. J. 1984; Genomic alterations associated with persistent infections by equine infectious anaemia virus, a retrovirus. Journal of General Virology 65:1395–1399
    [Google Scholar]
  18. Plagemann P. G. W., Harty J. T., Even C. 1992; Mode of neutralization of lactate dehydrogenase-elevating virus by polyclonal and monoclonal antibodies. Archives of Virology 123:89–100
    [Google Scholar]
  19. Rowson K. E. K., Mahy B. W. J. 1975; Laboratory methods. In Lactic dehydrogenase virus: Virology Monographs13 pp. 100–105 Gard S., Hallauer C. Edited by Wien & New York: Springer-Verlag;
    [Google Scholar]
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