1887

Abstract

Three out of 36 poliovirus type 1-specific monoclonal antibodies which, at 37 °C in a medium of normal ionic strength (μ = 0·16), caused only aggregative neutralization (reversible by immune complex dissociation at pH 2) shifted to cause disruptive, acid-irreversible neutralization when the temperature was raised to 39 °C or the ionic strength was lowered to 1/100 of normal. Under both conditions, the antigenic conversion was stoichiometric, but the efficiency was lower at 39 °C than at low ionic strength. Antigenic conversion and irreversible neutralization under both conditions were inhibited by WIN 51711, a capsid-stabilizing compound. Complete inhibition required filling of most of the virion’s binding pockets by this compound.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-3-581
1994-03-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/3/JV0750030581.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-3-581&mimeType=html&fmt=ahah

References

  1. Baxt B., Morgan D. O., Robertson B. H., Timpone C. A. 1984; Epitopes on foot-and-mouth disease virus outer capsid protein VP1 involved in neutralization and cell attachment. Journal of Virology 51:298–305
    [Google Scholar]
  2. Brioen P., Dekegel D., Boeyé A. 1983; Neutralization of poliovirus by antibody-mediated polymerization. Virology 127:463–468
    [Google Scholar]
  3. Brioen P., Rombaut B., Boeyé A. 1985; Hit-and-run neutralization of poliovirus. Journal of General Virology 66:2495–2499
    [Google Scholar]
  4. Chapman M. S., Minor I., Rossmann M. G., Diana G. D., Andries K. 1991; Human rhinovirus 14 complexed with antiviral compound R 61877. Journal of Molecular Biology 217:455–463
    [Google Scholar]
  5. Delaet I., Boeyé A. 1993; Monoclonal antibodies that disrupt poliovirus only at fever temperatures. Journal of Virology 67:5299–5302
    [Google Scholar]
  6. Delaet I., Vrijsen R., Boeyé A. 1992; Antigenic N to H conversion of poliovirus by a monoclonal antibody at low ionic strength. Virology 188:93–101
    [Google Scholar]
  7. Everaert L., Vrijsen R., Boeyé A. 1989; Eclipse products of poliovirus after cold-synchronized infection of HeLa cells. Virology 171:76–82
    [Google Scholar]
  8. Hogle J. M., Chow M., Filman D. J. 1985; Three-dimensional structure of poliovirus at 2·9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  9. Mccullough K. C. 1986; Monoclonal antibodies, implications for virology. Archives of Virology 87:1–36
    [Google Scholar]
  10. Mccullough K. C., Smale C. J., Carpenter W. C., Crowther J. R., Brocchi E., De Simone F. 1987; Conformational alteration in foot-and-mouth disease virus virion capsid structure after complexing with monospecific antibody. Immunology 60:75–82
    [Google Scholar]
  11. Outlaw M. C., Dimmock N. J. 1991; Insights into neutralization of animal viruses gained from study of influenza virus. Epidemiology and Infection 106:205–220
    [Google Scholar]
  12. Paul A. V., Schultz A., Pincus S. E., Oroszlan S., Wimmer E. 1987; Capsid protein VP4 of poliovirus is N-myristoylated. Proceedings of the National Academy of Sciences, U.S.A 84:7827–7831
    [Google Scholar]
  13. Rombaut B., Boeyé A. 1991; In vitro assembly of poliovirus 14S subunits: disoxaril stabilization as a model for the antigenicity conferring activity of infected cell extracts. Virology 180:788–792
    [Google Scholar]
  14. Rombaut B., Vrijsen R., Boeyé A. 1985; Stabilization by host cell components and Mg2+ of the neutralization epitopes of poliovirus. Journal of General Virology 66:303–307
    [Google Scholar]
  15. Rombaut B., Andries K., Boeyé A. 1991; A comparison of WIN 51711 and R 78206 as stabilizers of poliovirus virions and procapsids. Journal of General Virology 72:2153–2157
    [Google Scholar]
  16. Smith T. J., Kremer M. J., Luo M., Vriend G., Arnold E., Kamer G., Rossmann M. G., Mckinlay M. A., Diana G. D., Otto M. J. 1986; The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating. Science 233:1286–1293
    [Google Scholar]
  17. Thomas A. A. M., Brioen P., Boeyé A. 1985; A monoclonal antibody that neutralizes poliovirus by cross-linking virions. Journal of Virology 54:7–13
    [Google Scholar]
  18. Thomas A. A. M., Vrijsen R., Boeyé A. 1986; Relationship between poliovirus neutralization and aggregation. Journal of Virology 59:479–485
    [Google Scholar]
  19. Vrijsen R., Rombaut B., Boeyé A. 1983; A simple quantitative protein A micro-immunoprecipitation method; assay of antibodies to the N and H antigens of poliovirus. Journal of Immunological Methods 59:217–220
    [Google Scholar]
  20. Vrijsen R., Mosser A., Boeyé A. 1993; Postadsorption neutralization of poliovirus. Journal of Virology 67:3126–3133
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-3-581
Loading
/content/journal/jgv/10.1099/0022-1317-75-3-581
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error