1887

Abstract

Experimental infection of transmissible mink encephalopathy (TME) in two closely related mustelids, black ferret () and mink (revealed differences in their susceptibility to the TME agent. When challenged with the Stetsonville TME agent, a longer incubation period was observed in ferrets (28 to 38 months) than mink (4 months). Western blot analysis of ferret and mink prion proteins (PrP) demonstrated no detectable differences between the proteins. Northern blot analysis of ferret brain RNA indicated that PrP mRNA abundance is similar in infected and uninfected individuals. We amplified the PrP coding region from ferret DNA using the polymerase chain reaction and compared the deduced amino acid sequence of the ferret PrP gene with the mink PrP gene. This comparison revealed six silent base changes and two amino acid changes between mink and ferret: Phe → Lys at codon 179 and Arg → Gin at codon 224, respectively. These changes may indicate the region of PrP that is responsible for the species barrier effect between mink and ferret.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-11-2947
1994-11-01
2022-08-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/11/JV0750112947.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-11-2947&mimeType=html&fmt=ahah

References

  1. Bessen R. A., Marsh R. F. 1992; Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agent. Journal of Virology 66:2096–2101
    [Google Scholar]
  2. Blake M., Johnston K. H., Russel-Jones G. J., Gotschlich E. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Analytical Biochemistry 136:175–179
    [Google Scholar]
  3. Bolton D. C., Bendheim P. E., Marmorstein A. D., Potempska A. 1987; Isolation and structural studies on the intact scrapie agent protein. Archives of Biochemistry and Biophysics 258:579–590
    [Google Scholar]
  4. Büeler H., Fischer M., Lang Y., Bluethmann H., Lipp H. -P., Dearmond S. J., Prusiner S. B., Aguet M., Weissmann C. 1992; Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein. Nature; London: 356577–582
    [Google Scholar]
  5. Büeler H., Aguzzi A., Sailer A., Greiner R. -A., Autenried P., Aguet M., Weissmann C. 1993; Mice devoid of PrP are resistant to scrapie. Cell 13:1339–1347
    [Google Scholar]
  6. Carlson G. A., Goodman P. A., Lovett M., Taylor B. A., Marshall S. T., Peterson-Torchia M., Westaway D., Prusiner S. B. 1988; Genetics and polymorphism of the mouse prion gene complex: the control of scrapie incubation time. Molecular and Cellular Biology 8:5528–5540
    [Google Scholar]
  7. Chesebro B. 1990; Spongiform encephalopathies: the transmissible agents. In Virology pp 2325–2336 Fields B. N., Knipe D. M., Chanock R. M., Hirsch M. S., Melnick J. L., Monath T. P., Roizman B. Edited by New York: Raven Press;
    [Google Scholar]
  8. Chesebro B., Race R., Wehrly K., Nishio J., Bloom M., Lechner D., Bergstrom S., Robbins K., Mayer L., Keith J. M., Garron C., Haase A. 1985; Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain. Nature; London: 315331–333
    [Google Scholar]
  9. Dickinson A. G., Meikle V. M. H. 1971; Host-genotype and agent effects in scrapie incubation: change in allelic interaction with different strains of agent. Molecular and General Genetics 112:73–79
    [Google Scholar]
  10. Dickinson A. G., Meikle V. M. H., Fraser H. 1968; Identification of a gene which controls the incubation period of some strains of scrapie agent in mice. Journal of Comparative Pathology 78:293–299
    [Google Scholar]
  11. Goldfarb L. G., Korczyn A., Brown P., Chapman J., Gadusek D. C. 1990; Mutation in codon 200 of scrapie amyloid precursor gene linked to Creutzfeldt-Jakob disease in Sephardic Jews of Libyan and non-Libyan origin. Lancet 336:637–638
    [Google Scholar]
  12. Goldmann W., Hunter N., Foster J. D., Salbaum J. M., Beyreuther K., Hope J. 1990; Two alleles of a neural protein gene linked to scrapie in sheep. Proceedings of the National Academy of Sciences U.S.A: 872476–2480
    [Google Scholar]
  13. Goldmann W., Hunter N., Benson G., Foster J. D., Hope J. 1991a; Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip gene. Journal of General Virology 72:2411–2417
    [Google Scholar]
  14. Goldmann W., Hunter N., Martin T. E., Dawson M., Hope J. 1991b; Different forms of the bovine PrP gene have five of six copies of a short, G-C rich element within the protein-coding exon. Journal of General Virology 72:201–204
    [Google Scholar]
  15. Hsiao K. K., Baker H. F., Crow T. J., Poulter M., Owen F., Terwilliger J. D., Westaway D., Ott J., Prusiner S. B. 1989; Linkage of a prion protein missense variant to Gerstmann- Sträussler syndrome. Nature; London: 338342–345
    [Google Scholar]
  16. Hunter N., Hope J., Mcconnell I., Dickinson A. G. 1987; Linkage of the scrapie-associated fibril protein (PrP) gene and Sine using congenic mice and restriction fragment length polymorphism analysis. Journal of General Virology 68:2711–2716
    [Google Scholar]
  17. Hunter N., Foster J. D., Benson G., Hope J. 1991; Restriction fragment length polymorphisms of the scrapie-associated fibril protein (PrP) gene and their association with susceptibility to natural scrapie in British sheep. Journal of General Virology 72:1287–1292
    [Google Scholar]
  18. Kimberlin R. H., Cole S., Walker C. A. 1987; Temporary and permanent modifications to a single strain of mouse scrapie on transmission to rats and hamsters. Journal of General Virology 68:1875–1881
    [Google Scholar]
  19. Kimberlin R. H., Walker C. A., Fraser H. 1989; The genomic identity of different strains on mouse scrapie is expressed in hamsters and preserved on reisolation in mice. Journal of General Virology 70:2017–2025
    [Google Scholar]
  20. Kretzschmar H. A., Stowring L. E., Westaway D., Stubblebine W. H., Prusiner S. B., Dearmond S. J. 1986; Molecular cloning of a human prion protein cDNA. DNA 5:315–324
    [Google Scholar]
  21. Kretzschmar H. A., Neumann M., Riethmüller G., Prusiner S. B. 1992; Molecular cloning of a mink prion protein gene. Journal of General Virology 73:2757–2761
    [Google Scholar]
  22. Liao Y. -C., Tokes Z., Lim E., Lackey A., Woo C. H., Button J. D., Clawson G. A. 1987; Cloning of rat ‘prion-related protein’ cDNA. Laboratory Investigation 57:370–374
    [Google Scholar]
  23. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  24. Locht C., Chesebro B., Race R., Keith J. M. 1986; Molecular cloning and complete sequence of prion protein cDNA from mouse brain infected with the scrapie agent. Proceedings of the National Academy of Sciences U.S.A: 836372–6376
    [Google Scholar]
  25. Mckinley M. P., Prusiner S. B. 1986; Biology and structure of scrapie prions. International Review of Neurobiology 28:1–57
    [Google Scholar]
  26. Marsh R. F., Hanson R. P. 1969; Physical and chemical properties of the transmissible mink encephalopathy agent. Journal of Virology 3:176–181
    [Google Scholar]
  27. Marsh R. F., Bessen R. A., Lehmann S., Hartsough G. R. 1991; Epidemiological and experimental studies on a new incident of transmissible spongiform encephalopathy. Journal of General Virology 72:589–594
    [Google Scholar]
  28. Oesch B., Westaway D., Wälchli M., Mckinley M. P., Kent S. B. H., Aebersold R., Barry R. A., Tempst P., Teplow D. B., Hood L. E., Prusiner S. B., Weissmann C. 1985; A cellular gene encodes scrapie PrP 27–30 protein. Cell 40:735–746
    [Google Scholar]
  29. Pattison I. H. 1966; The relative susceptibility of sheep, goats and mice to two types of the goat scrapie agent. Research in Veterinary Science 7:207–212
    [Google Scholar]
  30. Prusiner S. B. 1982; Novel proteinaceous infectious particles cause scrapie. Science 216:136–144
    [Google Scholar]
  31. Prusiner S. B. 1991; Molecular biology of prion diseases. Science 252:1515–1522
    [Google Scholar]
  32. Prusiner S. B. 1992; Chemistry and biology of prions. Biochemistry 31:12277–12288
    [Google Scholar]
  33. Rohwer R. G. 1991; The scrapie agent: ‘a virus by any other name’. Current Topics in Microbiology and Immunology 172:195–232
    [Google Scholar]
  34. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Scott M., Foster D., Mirenda C., Serban D., Coufal F., Wälchli M., Torchia M., Groth D., Carlson G., Dearmond S. J., Westaway D., Prusiner S. B. 1989; Transgenic mice expressing hamster prion protein produce species-specific scrapie infectivity and amyloid plaques. Cell 59:847–857
    [Google Scholar]
  36. Scott M., Groth D., Foster D., Torchia M., Yang S. -L., Dearmond S. J., Prusiner S. B. 1993; Propagation of prions with artificial properties in transgenic mice expressing chimeric PrP genes. Cell 73:979–988
    [Google Scholar]
  37. Serban D., Taraboulos A., Dearmond S. J., Prusiner S. B. 1990; Rapid detection of Creutzfeldt-Jakob disease and scrapie prion proteins. Neurology 40:110–117
    [Google Scholar]
  38. Starr L., Quaranta V. 1992; An efficient and reliable method for cloning PCR-amplification products: a survey of point mutations in integrin cDNA. Biotechniques 13:612–618
    [Google Scholar]
  39. Weissmann C. 1991; A ‘unified theory’ of prion propagation. Nature; London: 352679–683
    [Google Scholar]
  40. Westaway D., Goodman P. A., Mirenda C. A., Mckinley M. P., Carlson G. A., Prusiner S. B. 1987; Distinct prion proteins in short and long scrapie incubation period mice. Cell 51:651–662
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-11-2947
Loading
/content/journal/jgv/10.1099/0022-1317-75-11-2947
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error