1887

Abstract

It has been demonstrated that the hepatocyte nuclear factor 1 (HNF1) binding site is critical for the majority of the hepatitis B virus (HBV) large surface antigen promoter activity in differentiated hepatoma cell lines. Examination of a series of clustered point mutations in the minimal large surface antigen promoter demonstrated that the HNF1 and TATA box binding sites are the major regulatory elements required for transcription from this promoter. Synthetic promoter constructs containing the large surface antigen promoter HNF1 binding site and TATA box element upstream of the luciferase open reading frame were tested for their transcriptional activities in HepG2.1 cells in the absence or presence of an HNF1 expression vector. These synthetic promoter constructs displayed a similar level of transcriptional activity and induction by HNF1 in comparison with the full-length large surface antigen promoter, suggesting that additional HBV sequences are dispensable for full transcriptional activity. The distance between the HNF1 binding site and TATA box element in the synthetic promoter constructs appeared to influence the transcriptional activity modestly and in a periodic manner.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-10-2671
1994-10-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/10/JV0750102671.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-10-2671&mimeType=html&fmt=ahah

References

  1. Antonucci T. K., Rutter W. J. 1989; Hepatitis B virus (HBV) promoters are regulated by the HBV enhancer in a tissue-specific manner. Journal of Virology 63:579–583
    [Google Scholar]
  2. Araki K., Miyazaki J.-I., Hino O., Tomita N., Chisaka O., Matsubara K., Yamamura K.-I. 1989; Expression and replication of hepatitis B virus genome in transgenic mice. Proceedings of the National Academy of Sciences, U.S.A 86:207–211
    [Google Scholar]
  3. Baumhueter S., Courtois G., Crabtree G. R. 1988; A variant nuclear protein in dedifferentiated hepatoma cells binds to the same functional sequences in the β fibrinogen gene promoter as HNF1. EMBO Journal 7:2485–2493
    [Google Scholar]
  4. Briggs M. R., Kadonaga J. T., Bell S. P., Tjian R. 1986; Purification and biochemical characterization of the promoter-specific transcription factor, SP1. Science 234:47–52
    [Google Scholar]
  5. Bruss V., Ganem D. 1991; The role of envelope proteins in hepatitis B virus assembly. Proceedings of the National Academy of Sciences, U.S.A 88:1059–1063
    [Google Scholar]
  6. Cereghini S., Yaniv M., Cortese R. 1990; Hepatocyte dedifferentiation and extinction is accompanied by a block in the synthesis of mRNA coding for the transcription factor HNF1/LFB1. EMBO Journal 9:2257–2263
    [Google Scholar]
  7. Chang C., Jeng K.-S., Hu C.-P., Lo S. J., Su T.-S., Ting L.-P., Chou C.-K., Han S.-H., Pfaef E., Salfeld J., Schaller H. 1987; Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line. EMBO Journal 6:675–680
    [Google Scholar]
  8. Chang H.-K., Wang B.-Y., Yuh C.-H., Wei C.-L., Ting L.-P. 1989; A liver-specific nuclear factor interacts with the promoter region of the large surface protein gene of human hepatitis B virus. Molecular and Cellular Biology 9:5189–5197
    [Google Scholar]
  9. Cohen R. S., Meselson M. 1988; Periodic interactions of heat shock transcriptional elements. Nature; London: 332856–858
    [Google Scholar]
  10. Courtois G., Morgan J. G., Campbell L. A., Fourel G., Crabtree G. R. 1987; Interaction of a liver-specific nuclear factor with the fibrinogen and α1-antitrypsin promoters. Science 238:688–692
    [Google Scholar]
  11. Courtois G., Baumhueter S., Crabtree G. R. 1988; Purified hepatocyte nuclear factor 1 interacts with a family of hepatocyte-specific promoters. Proceedings of the National Academy of Sciences, U.S.A 85:7937–7941
    [Google Scholar]
  12. De-Medina T., Faktor O., Shaul Y. 1988; The S promoter of hepatitis B virus is regulated by positive and negative elements. Molecular and Cellular Biology 8:2449–2455
    [Google Scholar]
  13. De Wet J. R., Wood K. V., Deluca M., Helinski D. R., Subramani S. 1987; Firefly luciferase gene: structure and expression in mammalian cells. Molecular and Cellular Biology 7:725–737
    [Google Scholar]
  14. Dignam J. D., Lebovitz R. M., Roeder R. G. 1983; Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Research 11:1475–1489
    [Google Scholar]
  15. Dubois M. F., Pourcel C., Rousset S., Chany C., Tiollais P. 1980; Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA. Proceedings of the National Academy of Sciences, U.S.A 77:4549–4553
    [Google Scholar]
  16. Farza H., Hadchouel M., Scotto I., Tiollais P., Babinet C., Pourcel C. 1988; Replication and gene expression of hepatitis B virus in a transgenic mouse that contains the complete viral genome. Journal of Virology 62:4144–4152
    [Google Scholar]
  17. Frain M., Swart G., Monaci P., Nicosia A., Stampfil S., Frank R., Cortese R. 1989; The liver-specific transcription factor LF- B1 contains a highly diverged homeobox DNA binding domain. Cell 59:145–157
    [Google Scholar]
  18. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyl-transferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  19. Graham F. L., van der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  20. Honigwachs J., Faktor O., Dikstein R., Shaul Y., Laub O. 1989; Liver-specific expression of hepatitis B virus is determined by the combined action of the core gene promoter and the enhancer. Journal of Virology 63:919–924
    [Google Scholar]
  21. Iameel S., Siddiqui A. 1986; The human hepatitis B virus enhancer requires trans-acting cellular factor(s) for activity. Molecular and Cellular Biology 6:710–715
    [Google Scholar]
  22. Karpen S., Banerjee R., Zelent A., Price P., Acs G. 1988; Identification of protein-binding sites in the hepatitis B virus enhancer and core promoter domains. Molecular and Cellular Biology 8:5159–5165
    [Google Scholar]
  23. Landt O., Grunert H. P., Hahn U. 1990; A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene 96:125–128
    [Google Scholar]
  24. Lichtsteiner S., Schibler U. 1989; A glycosylated liver-specific transcription factor stimulates transcription of the albumin gene. Cell 57:1179–1187
    [Google Scholar]
  25. Mclachlan A., Milich D. R., Raney A. K., Riggs M. G., Hughes J. L., Sorge J., Chisari F. V. 1987; Expression of hepatitis B virus surface and core antigens: influences of pre-S and precore sequences. Journal of Virology 61:683–692
    [Google Scholar]
  26. Nakabayashi H., Taketa K., Miyano K., Yamane T., Sato J. 1982; Growth of human hepatoma cell lines with differentiated functions in chemically defined medium. Cancer Research 42:3858–3863
    [Google Scholar]
  27. Nakao K., Miyao Y., Ohe Y., Tamaoki T. 1989; Involvement of an AFPl-binding site in cell-specific transcription of the pres-S1 region of the human hepatitis B virus surface antigen gene. Nucleic Acids Research 17:9833–9842
    [Google Scholar]
  28. Ou J.-H., Rutter W. J. 1987; Regulation of secretion of the hepatitis B virus major surface antigen by the PreS-1 protein. Journal of Virology 61:782–786
    [Google Scholar]
  29. Patel N. U., Jameel S., Isom H., Siddiqui A. 1989; Interactions between nuclear factors and the hepatitis B virus enhancer. Journal of Virology 63:5293–5301
    [Google Scholar]
  30. Persing D. H., Varmus H. E., Ganem D. 1986; Inhibition of secretion of hepatitis B surface antigen by a related presurface polypeptide. Science 234:1388–1391
    [Google Scholar]
  31. Pourcel C., Louis A., Gervais M., Chenciner N., Dubois M.-F., Tiollais P. 1982; Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA. Journal of Virology 42:100–105
    [Google Scholar]
  32. Puck T. T., Marcus P. I., Cieciura S. J. 1956; Clonal growth of mammalian cells in vitro: growth characteristics of colonies from single HeLa cells with and without a ‘feeder’ layer. Journal of Experimental Medicine 103:273–284
    [Google Scholar]
  33. Raney A. K., Mclachlan A. 1991; The biology of hepatitis B virus. In Molecular Biology of the Plepatitis B Virus pp. 1–37 Mclachlan A. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  34. Raney A. K., Milich D. R., Mclachlan A. 1989; Characterization of hepatitis B virus major surface antigen gene transcriptional regulatory elements in differentiated hepatoma cell lines. Journal of Virology 63:3919–3925
    [Google Scholar]
  35. Raney A. K., Milich D. R., Easton A. J., Mclachlan A. 1990; Differentiation specific transcriptional regulation of the hepatitis B virus large surface antigen gene in human hepatoma cell lines. Journal of Virology 64:2360–2368
    [Google Scholar]
  36. Raney A. K., Easton A. J., Milich D. R., Mclachlan A. 1991; Promoter-specific transactivation of hepatitis B virus transcription by a glutamine- and proline-rich domain of hepatocyte nuclear factor 1. Journal of Virology 65:5774–5781
    [Google Scholar]
  37. Raney A. K., Le H. B., Mclachlan A. 1992; Regulation of transcription from the hepatitis B virus major surface antigen promoter by the Sp1 transcription factor. Journal of Virology 66:6912–6921
    [Google Scholar]
  38. Rey-Campos J., Chouard T., Yaniv M., Cereghini S. 1991; vHNF1 is a homeoprotein that activates transcription and forms heterodimers with HNF1. EMBO Journal 10:1445–1457
    [Google Scholar]
  39. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  40. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  41. Sells M. A., Chen M.-L., Acs G. 1987; Production of hepatitis B virus particles in HepG2 cells transfected with cloned hepatitis B virus DNA. Proceedings of the National Academy of Sciences, U.S.A 84:1005–1009
    [Google Scholar]
  42. Shaul Y., Ben Levy R. 1987; Multiple nuclear proteins in liver cells are bound to hepatitis B virus enhancer element and its upstream sequences. EMBO Journal 6:1913–1920
    [Google Scholar]
  43. Shaul Y., Rutter W. J., Laub O. 1985; A human hepatitis B viral enhancer element. EMBO Journal 4:427–430
    [Google Scholar]
  44. Siddiqui A., Jameel S., Mapoles J. 1986; Transcriptional control elements of hepatitis B surface antigen gene. Proceedings of the National Academy of Sciences, U.S.A 83:566–570
    [Google Scholar]
  45. Sorge J., Wright D., Erdman V. D., Cutting A. E. 1984; Amphotropic retrovirus vector system for human cell gene transfer. Molecular and Cellular Biology 4:1730–1737
    [Google Scholar]
  46. Sureau C., Romet-Lemonne J.-L., Mullins J. I., Essex M. 1986; Production of hepatitis B virus by a differentiated human hepatoma cell line after transfection with cloned circular HBV DNA. Cell 47:31–41
    [Google Scholar]
  47. Tognoni A., Cattaneo R., Serfling E., Schaffner W. 1985; A novel expression selection approach allows precise mapping of the hepatitis B virus enhancer. Nucleic Acids Research 13:7457–7472
    [Google Scholar]
  48. Tsurimoto T., Fujiyama A., Matsubara K. 1987; Stable expression and replication of hepatitis B virus genome in an integrated state in a human hepatoma cell line transfected with the cloned viral DNA. Proceedings of the National Academy of Sciences, U.S.A 84:444–448
    [Google Scholar]
  49. Ueda K., Tsurimoto T., Matsubara K. 1991; Three envelope proteins of hepatitis B virus: large S, middle S, and major S proteins needed for the formation of Dane particles. Journal of Virology 65:3521–3529
    [Google Scholar]
  50. Yaginuma K., Shirakata Y., Kobayashi M., Koike K. 1987; Hepatitis B virus (HBV) particles are produced in a cell culture system by transient expression of transfected HBV DNA. Proceedings of the National Academy of Sciences, U.S.A 84:2678–2682
    [Google Scholar]
  51. Yee J.-K. 1989; A liver-specific enhancer in the core promoter region of human hepatitis B virus. Science 246:658–661
    [Google Scholar]
  52. Yuh C.-H., Ting L.-P. 1993; Differentiated liver cell specificity of the second enhancer of hepatitis B virus. Journal of Virology 67:142–149
    [Google Scholar]
  53. Zhou D.-X., Yen T. S. B. 1991; The ubiquitous transcription factor Oct-1 and the liver-specific factor HNF-1 are both required to activate transcription of a hepatitis B virus promoter. Molecular and Cellular Biology 11:1353–1359
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-10-2671
Loading
/content/journal/jgv/10.1099/0022-1317-75-10-2671
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error