1887

Abstract

Persistent infection with a variant of influenza C/Ann Arbor/1/50 virus in MDCK cells has been previously reported. However, the precise molecular mechanism of persistence is still unknown. We show that the release of active progeny virus, as tested for by haemagglutination and acetylesterase profiles, does not take place in freshly seeded MDCK cells. Productive virus replication occurs simultaneously with massive production of structural proteins as shown by immunoprecipitation and immunofluorescence. PCR for the HEF structural protein-encoding segment 4 revealed that positive-sense RNA is present only during virus multiplication whereas negative-sense RNA appears to be constantly detectable. In this study we give initial evidence that influenza C virus can persist in the form of its genomic minus strand RNA, and plus strand transcription, protein synthesis and virus replication remain restricted to productive phases.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-9-2019
1993-09-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/9/JV0740092019.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-9-2019&mimeType=html&fmt=ahah

References

  1. Borzakian S, Couderc T, Barbier Y, Attal G, Pelletier I, Colbère-Garapin F. Persistent poliovirus infection: Establishment and maintenance involve distinct mechanisms. Virology 186:398–408 1992
    [Google Scholar]
  2. Buonagurio D, Nakada S, Desselberger U, Krystal M, Palese P. Noncumulative sequence changes in the hemagglutinin genes of influenza C virus isolates. Virology 146:221–232 1985
    [Google Scholar]
  3. Camilleri JJ, Maassab HF. Characteristics of a Persistent Infection in Madin-Darby Canine Kidney Cells with Influenza C Virus. Intervirology 29:178–184 1988
    [Google Scholar]
  4. Celma ML, Fernandez-Munoz R. Measles virus gene expression in lytic and persistent infections of a human lymphoblastoid cell line. Journal of General Virology 73:2203–2209 1992
    [Google Scholar]
  5. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159 1987
    [Google Scholar]
  6. Frielle DW, Huang DD, Youngner JS. Persistent infection with influenza A virus: Evolution of virus mutants. Virology 138:103–117 1984
    [Google Scholar]
  7. Goshima Y, Maeno K. Persistent Infection of MDCK Cells by Influenza C Virus: Initiation and Characterization. Journal of General Virology 70:3481–3485 1989
    [Google Scholar]
  8. Herrler G, Klenk HD. Structure and Function of the Hef Glycoprotein of Influenza C Virus. Advances in Virus Research 40:213–234 1991
    [Google Scholar]
  9. Levine B, Griffin DE. Persistence of viral RNA in mouse brains after recovery from acute alphavirus encephalitis. Journal of Virology 66:6429–6435 1992
    [Google Scholar]
  10. Manuguerra JC, Hannoun C, Nicolson C, Robertson JS. Genic amplification of the entire coding region of the HEF RNA segment of influenza C virus. Journal of Virological Methods 41:59–76 1993
    [Google Scholar]
  11. Marschall M, Leser U, Seibl R, Wolf H. Identification of proteins encoded by Epstein-Barr virus trans-activator genes. Journal of Virology 63:938–942 1989
    [Google Scholar]
  12. Montgomery LB, Kao CY, Verdin E, Cahill C, Maratos-Flier E. Infection of a polarized epithelial cell line with wild-type reovirus leads to virus persistence and altered cellular function. Journal of General Virology 72:2939–2946 1991
    [Google Scholar]
  13. Oldstone M. Molecular anatomy of viral persistence.. Journal of Virology 65:6381–6386 1991
    [Google Scholar]
  14. Smith F, Palese P. Variation in influenza virus genes: epidemiological, pathogenic and evolutionary consequences. Edited by Krug R. M. The Influenza Viruses319–350 1989 Plenum Press; New York: pp In
    [Google Scholar]
  15. Umetsu Y, Sugawara K, Nishimura H, Hongo S, Matsuzaki M, Kitame F, Nakamura K. Selection of antigenically distinct variants of influenza C viruses by the host cell. Virology 189:740–744 1992
    [Google Scholar]
  16. Urabe M, Tanaka T, Odagiri T, Tashiro M, Tobita K. Persistence of viral genes in a variant of MDBK cell after productive replication of a mutant of influenza virus A/WSN. Archives of Virology 128:97–110 1993
    [Google Scholar]
  17. Zack JA, Arrigo SJ, Weitsman SR, Go AS, Haislip A, Chen ISY. HIV-1 entry into quiescent primary lymphocytes: Molecular analysis reveals a labile, latent viral structure. Cell 61:213–222 1990
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-9-2019
Loading
/content/journal/jgv/10.1099/0022-1317-74-9-2019
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error