1887

Abstract

The genomic RNA sequence of tobamovirus Ob (Ob), which can spread systemically in tobacco carrying the N gene, was determined. It consists of 6507 nucleotides and contains four open reading frames, exactly corresponding to the genomic organization of tobamoviruses known so far, i.e. encoding the 130K, 180K, 30K and coat proteins. There were no nucleotide overlaps between any open reading frames. The Ob nucleic acid sequence, predicted protein sequences and gene organization were compared with those of other tobamoviruses reported previously. This virus was originally reported as a tomato mosaic virus; however, the nucleotide sequence data given here refute this classification. The determinants that allow tobamovirus Ob to overcome the N gene, a feature peculiar to this virus, were not identified apart from sequence data. This virus should be regarded as a new tobamovirus. The determinants interacting with the tentative N gene product have not yet been analysed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-9-1939
1993-09-01
2024-05-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/9/JV0740091939.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-9-1939&mimeType=html&fmt=ahah

References

  1. Ahlquist P, Strauss EG, Rice CM, Strauss JH, Haseloff J, Zimmern D. 1985; Sindbis virus proteins nsPl and nsP2 contain homology to nonstructural proteins from several RNA plant viruses.. Journal of Virology 53:536–542
    [Google Scholar]
  2. Alonso E, Garcia-Luque I, de la Cruz A, Wicke B, Avila-Rincon MJ, Serra MT, Castresana C, Diaz-Ruiz JR. 1991; Nucleotide sequence of the genomic RNA of pepper mild mosaic virus, a resistance-breaking tobamovirus in pepper.. Journal of General Virology 72:2875–2884
    [Google Scholar]
  3. Csilléry G, Tobias I, Rusko J. 1983; A new pepper strain of tomato mosaic virus.. Acta phytopathologica Academiae scientiarum hungaricae 18:195–200
    [Google Scholar]
  4. Deom CM, Oliver MJ, Beachy RN. 1987; The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement.. Science 237:389–394
    [Google Scholar]
  5. Goelet P, Lomonossoff GP, Butler PJG, Akam ME, Gait MJ, Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA.. Proceedings of the National Academy of Sciences, U.S.A. 79:5818–5822
    [Google Scholar]
  6. Haseloff J, Goelet P, Zimmern D, Ahlquist P, Dasgupta R, Kaesberg P. 1984; Striking similarities in amino acid sequence among non-structural proteins encoded by RNA viruses that have dissimilar genomic organization.. Proceedings of the National Academy of Sciences, U.S.A. 81:4358–4362
    [Google Scholar]
  7. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.. Gene 28:351–359
    [Google Scholar]
  8. Ishikawa M, Meshi T, Motoyoshi F, Takamatsu N, Okada Y. 1986; In vitro mutagenesis of the putative replicase genes of tobacco mosaic virus.. Nucleic Acids Research 14:8291–8305
    [Google Scholar]
  9. Kamer G, Argos P. 1984; Primary structural comparison of RNA-dependent polymerases from plant, animal and bacteria viruses.. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  10. Matthews REF. 1991 Plant Virology, 3rd edn.. pp. 644–645 New York: Academic Press;
    [Google Scholar]
  11. Meshi T, Takamatsu N, Ohno T, Okada Y. 1982; Molecular cloning of the complementary DNA copies of the common and cowpea strain of tobacco mosaic virus RNA.. Virology 118:64–75
    [Google Scholar]
  12. Meshi T, Watanabe Y, Saito T, Sugimoto A, Maeda T, Okada Y. 1987; Function of the 30Kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication.. EMBO Journal 6:2557–2563
    [Google Scholar]
  13. Meshi T, Motoyoshi F, Maeda T, Yoshikawa S, Watanabe H, Okada Y. 1989; Mutations in the tobacco mosaic virus 30kD protein gene overcome Tm-2 resistance in tomato.. Plant Cell 1:515–522
    [Google Scholar]
  14. Ohno T, Aoyagi M, Yamanashi Y, Saito H, Ikawa S, Meshi T, Okada Y. 1984; Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome.. Journal of Biochemistry 96:1915–1923
    [Google Scholar]
  15. Okada Y. 1986; Molecular assembly of tobacco mosaic virus in vitro.. Advances in Biophysics 22:95–149
    [Google Scholar]
  16. Osbourn JK, Sarkar S, Wilson TMA. 1990; Complementation of coat protein-defective TMV mutants in transgenic tobacco plants expressing TMV coat protein.. Virology 179:921–925
    [Google Scholar]
  17. Otsuki Y, Takebe I, Ohno T, Fukuda M, Okada Y. 1977; Reconstitution of tobacco mosaic virus rods occurs bidirectionally from an internal initiation region: demonstration by electron microscopic serology.. Proceedings of the National Academy of Sciences, U.S.A. 74:1913–1917
    [Google Scholar]
  18. Saito T, Yamanaka K, Watanabe Y, Takamatsu N, Meshi T, Okada Y. 1989; Mutational analysis of the coat protein gene of tobacco mosaic virus in relation to hypersensitive response in tobacco plants with the Nʹ gene.. Virology 173:11–20
    [Google Scholar]
  19. Sanger F, Nicklen S, Coulson AR. 1977; DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  20. Solis I, Garcia-Arenal F. 1990; The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus.. Virology 177:553–558
    [Google Scholar]
  21. Takamatsu N, Ohno T, Meshi T, Okada Y. 1983; Molecular cloning and nucleotide sequence of 30K and the coat protein cistron of TMV (tomato strain) genome.. Nucleic Acids Research 11:3767–3778
    [Google Scholar]
  22. Takamatsu N, Ishikawa M, Meshi T, Okada Y. 1987; Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV RNA.. EMBO Journal 6:307–311
    [Google Scholar]
  23. Ugaki M, Tomiyama M, Kakutani T, Hidaka S, Kiguchi T, Nagata R, Sato T, Motoyoshi F, Nishiguchi M. 1991; The complete nucleotide sequence of cucumber green mottle mosaic virus (SH strain) genomic RNA.. Journal of General Virology 72:1487–1495
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-9-1939
Loading
/content/journal/jgv/10.1099/0022-1317-74-9-1939
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error