1887

Abstract

The nucleotide sequence of 1.7 kbp cDNA, comprising the region nearest the 3′ end of the genome of the porcine epidemic diarrhoea virus (PEDV), has been independently determined for two European isolates of PEDV. Almost identical results were obtained for the two isolates, which were derived from cases of PEDV infection in Belgium and Britain in 1977 and 1987, respectively. The sequences contained a 1323 nucleotide (nt) open reading frame (ORF), which showed moderate identity to the nucleocapsid (N) gene of other coronaviruses. The greatest similarity at both the nucleic acid and protein levels was to the human coronavirus 229E. The PEDV N gene was, however, notably larger than that of the human 229E and porcine transmissible gastroenteritis viruses. This reflects the presence of a putative insertion of approximately 135 nt located towards the middle of the N gene. A second 336 nt ORF, which might encode a leucine-rich protein similar to, but shorter than, the bovine coronavirus internal protein was found within the PEDV N gene. Several RNA motifs typical of coronaviruses were also observed. These results confirm the earlier provisional classification of PEDV as a coronavirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-9-1795
1993-09-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/9/JV0740091795.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-9-1795&mimeType=html&fmt=ahah

References

  1. Altschul SF, Gish W, Miller W, Myers E.W, Lipman DJ. 1990; Basic local alignment search tool.. Journal of Molecular Biology 215:403–110
    [Google Scholar]
  2. Baric RS, Nelson GW, Fleming JO, Deans RJ, Casteel N, Stohlman SA. 1988; Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription.. Journal of Virology 62:4280–4287
    [Google Scholar]
  3. Boursnell MEG, Binns MM, Foulds IJ, Brown TDK. 1985; Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus.. Journal of General Virology 66:573–580
    [Google Scholar]
  4. Bridgen A, Tobler K, Ackermann M. 1993; Identification of coronaviral conserved sequences and application to viral genome amplification.. Advances in Experimental Biology and Medicine in press
    [Google Scholar]
  5. Britton P, Mawditt KL, Page KW. 1991; The cloning and sequencing of the virion protein genes from a British isolate of porcine respiratory coronavirus: comparison with transmissible gastroenteritis genes.. Virus Research 21:181–198
    [Google Scholar]
  6. Delmas B, Gelfi J, L’Haridon R, Vogel LK, Sjostrom H, Noren O, Laude H. 1992; Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV.. Nature, London 357:417–420
    [Google Scholar]
  7. Devereux j, Haeberli P, Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12:387–395
    [Google Scholar]
  8. Duarte M, Gelfi J, Lambert P, Rasschaert D, Laude H. 1993; Genome organisation of porcine epidemic diarrhea virus (PEDV).. Advances in Experimental Biology and Medicine in press
    [Google Scholar]
  9. Egberink HF, Ederveen J, Callebaut P, Horzinek MC. 1988; Characterization of the structural proteins of porcine epizootic diarrhea virus, strain CV 777.. American Journal of Veterinary Research 49:1320–1324
    [Google Scholar]
  10. Friedrich K, Brombach M, Pon CL. 1988; Identification, cloning and sequence of the Streptococcus faecium infB (translational initiation factor IF2) gene.. Molecular and General Genetics 214:595–600
    [Google Scholar]
  11. Frohman MA, Dush MK, Martin GR. 1988; Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.. Proceedings of the National Academy of Sciences, U.S.A. 85:8998–9002
    [Google Scholar]
  12. Garwes DJ, Stewart F, Britton P. 1989; The polypeptide of Mt 14000 of porcine transmissible gastroenteritis virus: gene assignment and intracellular location.. Journal of General Virology 70:2495–2499
    [Google Scholar]
  13. FIave P, Moving V, Svansson V, Uttenthal A, Bloch B. 1992; Coronavirus infection in mink (Mustela vison). Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus.. Veterinary Microbiology 31:1–10
    [Google Scholar]
  14. Hofmann MA, Brian DA. 1993; An intraleader open reading frame is selected from a hypervariable leader 5′ terminus during persistent infection by the bovine coronavirus.. Advances in Experimental Biology and Medicine in press
    [Google Scholar]
  15. Hofmann M, Wyler R. 1988; Propagation of the virus of porcine epidemic diarrhea in cell culture.. Journal of Clinical Microbiology 26:2235–2239
    [Google Scholar]
  16. Hofmann M, Wyler R. 1989; Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV).. Veterinary Microbiology 20:131–142
    [Google Scholar]
  17. Horsburgh BC, Brierley I, Brown TDK. 1992; Analysis of a 9-6 kb sequence from the 3′ end of canine coronavirus genomic RNA.. Journal of General Virology 73:2849–2862
    [Google Scholar]
  18. Johr L. 1989 Induktion und Charakterisierung von monoklonalen Antikorpern gegen das Nukleokapsidprotein des Virus der Epide-mischen Virusdiarrhoe der Schweine. Inaugural Dissertation zur Erlangung der Doktorwiirde der Veterinar-Medizinischen Fakultat der Universitat Zürich..
    [Google Scholar]
  19. Kamahora T, Soe LH, Soe LH. 1990; Integrin and other cell adhesion molecules.. Federation of American Societies for Experimental Biology Journal 4:2868–2880
    [Google Scholar]
  20. Kamahora T, Soe LH, Lai MMC. 1989; Sequence analysis of the nuclecapsid gene and leader RNA of human coronavirus OC43.. Virus Research 12:1–9
    [Google Scholar]
  21. Kapke PA, Brian DA. 1986; Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene.. Virology 151:41–49
    [Google Scholar]
  22. Kingston RE. 1991 Current Protocols in Molecular Biology. New York: Greene Publishing Associates.;
    [Google Scholar]
  23. Knuchel M, Ackermann M, Muller HK, Kihm U. 1992; An ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV) based on the specific solubility of the viral surface glycoprotein.. Veterinary Microbiology 32:117–134
    [Google Scholar]
  24. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.. Cell 44:283–292
    [Google Scholar]
  25. Kusanagi K, Kuwahara H, Katoh T, Nunoya T, Ishikawa Y, Samejima T, Tajima M. 1992; Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate.. Journal of Veterinary Medical Science 54:313–318
    [Google Scholar]
  26. Lai MMC. 1990; Coronavirus: organization, replication and expression of genome.. Annual Review of Microbiology 44:303–333
    [Google Scholar]
  27. Lapps W, Hogue BG, Brian DA. 1987; Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes.. Virology 157:47–57
    [Google Scholar]
  28. Masters PS, Sturman LS. 1990; Functions of the coronavirus nucleocapsid protein.. Advances in Experimental Medicine and Biology 276:235–238
    [Google Scholar]
  29. Page KW, Britton P, Boursnell MEG. 1990; Sequence analysis of the leader RNA of two porcine coronaviruses: transmissible gastroenteritis virus and porcine respiratory coronavirus.. Virus Genes 4:289–301
    [Google Scholar]
  30. Parker MM, Masters PS. 1990; Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain structure for the nucleocapsid protein.. Virology 179:463–168
    [Google Scholar]
  31. Pensaert MB. 1989; Porcine epidemic diarrhea virus.. Virus Infections of PorcinesEdited by Pensaert M. B. Amsterdam: Elsevier.; pp In
    [Google Scholar]
  32. Pensaert MB, Debouck P. 1978; A new coronavirus-like particle associated with diarrhea in swine.. Archives of Virology 58:243–247
    [Google Scholar]
  33. Pensaert MB, Debouck P, Reynolds DJ. 1981; An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses.. Archives of Virology 68:45–52
    [Google Scholar]
  34. Queen C, Korn LJ. 1984; Microgenie sequence analysis program.. Nucleic Acids Research 12:581–599
    [Google Scholar]
  35. Rasschaert D, Gelfi J, Laude H. 1987; Enteric coronavirus TGEV: partial sequence of the genomic RNA, its organization and expression.. Biochimie 69:591–600
    [Google Scholar]
  36. Rasschaert D, Duarte M, Laude H. 1990; Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions.. Journal of General Virology 71:2599–2607
    [Google Scholar]
  37. Sambrook J, Fritsch EF, Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  38. Schreiber SS, Kamahora T, Lai MMC. 1989; Sequence analysis of the nucleocapsid protein gene of human coronavirus 229E.. Virology 169:142–151
    [Google Scholar]
  39. Senanayake SD, Hofmann MA, Maki JL, Brian DA. 1992; The nucleocapsid protein gene of bovine coronavirus is bicistronic.. Journal of Virology 66:5277–5283
    [Google Scholar]
  40. Skinner MA, Siddell SG. 1983; Coronavirus JHM: nucleotide sequence of the mRNA that encodes nucleocapsid protein.. Nucleic Acids Research 11:5045–5054
    [Google Scholar]
  41. Tobler K, Bridgen A, Ackermann M. 1993; Sequence analysis of the nucleocapsid protein gene of porcine epidemic diarrhoea virus.. Advances in Experimental Biology and Medicine in press
    [Google Scholar]
  42. Tung FYT, Abraham S, Sethna M, Hung SL, Sethna P, Hogue BG, Brian DA. 1992; The 9-kda hydrophobic protein encoded at the 3′ end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated.. Virology 186:676–683
    [Google Scholar]
  43. Vennema H, de Groot RJ, Harbour DA, Horzinek MC, Spaan WJM. 1991; Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens.. Virology 181:327–335
    [Google Scholar]
  44. Vennema H, Rossen JWA, Wesseling J, Horzinek MC, Rottier PJM. 1992; Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses.. Virology 191:134–140
    [Google Scholar]
  45. Wirth UV, Vogt B, Schwyzer M. 1991; The three major immediate-early transcripts of bovine herpesvirus 1 arise from two divergent and spliced transcription units.. Journal of Virology 65:195–205
    [Google Scholar]
  46. Yaling Z, Ederveen J, Egberink H, Pensaert M, Horzinek MC. 1988; Porcine epidemic diarrhea virus (CV 777) and feline infectious peritonitis virus (FIPV) are antigenically related.. Archives of Virology 102:63–71
    [Google Scholar]
  47. Yeager CL, Ashmun RA, Williams RK, Cardellichio CB, Shapiro LH, Look AT, Holmes KV. 1992; Human aminopeptidase N is a receptor for human coronavirus 229E.. Nature, London 357:420–422
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-9-1795
Loading
/content/journal/jgv/10.1099/0022-1317-74-9-1795
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error