1887

Abstract

Transmissible spongi form encephalopathies are fatal neurodegenerative disorders which are linked to abnormal isoforms of the prion protein (PrP), which is expressed in different cells of various mammalian species. Susceptibility to disease and reduced transmission rates upon the first passage to another species are thought to be a result of functional and biochemical differences of the PrP as a consequence of amino acid sequence among species. In 1985 an epidemic of bovine spongiform encephalopathy (BSE) started after accidential transmission of scrapie by feeding infected sheep and goat meat and bone meal products to cattle. In this report we present data demonstrating speciesspecific epitopes in bovine, ovine and murine PrP that are based on amino acid substitutions at positions 108 and 110. Rabbit antisera to synthetic peptides representing amino acid sequence 108 to 123 of PrP of cattle, sheep and mice reacted strongly with modified PrP of the homologous host but not, or only poorly, with PrP of heterogeneous origin. Cross-reactivity was observed, however, with antisera to bovine and ovine peptide sequences 102 to 117, thus stressing the importance of the location of the amino acid substitution in synthetic peptides used for immunization. Based on these data, BSE PrP and ovine and murine scrapie PrP can be distinguished from each other, and these differences might help elucidate the species barrier effect.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-7-1451
1993-07-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/7/JV0740071451.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-7-1451&mimeType=html&fmt=ahah

References

  1. Asher DM, Gibbs CJ, Gajdusek DC. 1986; Slow viral infections: safe handling of the agents of subacute spongiform encephalopathies.. Edited by Miller BM. Laboratory Safety: Principles and Practices Washington, D.C.: American Society for Microbiology; In
    [Google Scholar]
  2. Bolton DC, Seligman SJ, Bablanian G, Windsor D, Scala LJ, Kim KS, Chen CM, Kascsak RJ, Bendheim PE. 1991; Molecular location of a species-specific epitope on the hamster scrapie agent protein. Journal of Virology 65:3667–3675
    [Google Scholar]
  3. Burnette WN. 1981; “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  4. Caughey B. 1991; In vitro expression and biosynthesis of prion protein. Current Topics in Microbiology and lmmunology 172:93–107
    [Google Scholar]
  5. Fraser H, Bruce ME, Mcconnell I. 1991; Murine scrapie strains, BSE models and genetics.. Edited by Bradley R, Savey M, Marchant B. Sub-acute Spongiform Encephalopathies: Proceedings of the CEC Agricultural Research Programme Seminar 1990, Dordrecht: Kluwer Academic Publishers.; pp In 131–136
    [Google Scholar]
  6. Goldfarb LG, Brown P, Vrbovská A, Baron H, McCombie WR, Cathala F, Gibbs CJ, Gajdusek DC. 1992; An insert mutation in the chromosome 20 amyloid precursor gene in a Gerstmann-Sträussler-Scheinker family. Journal of the Neurological Sciences 111:189–194
    [Google Scholar]
  7. Goldmann W, Hunter N, Foster JD, Salbaum JM, Beyreuther K, Hope J. 1990; Two alleles of a neural protein gene linked to scrapie in sheep.. Proceedings of the National Academy of Sciences U.S.A. 87:2476–2480
    [Google Scholar]
  8. Goldmann W, Hunter N, Martin T, Dawson M, Hope J. 1991; Two alleles of a neural protein gene linked to scrapie in sheep.. Proceedings of the National Academy of Sciences U.S.A. 72:201–204
    [Google Scholar]
  9. Hilmert H, Diringer H. 1984; A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters.. Bioscience Reports 4:165–170
    [Google Scholar]
  10. Kimberlin RH. 1991; Agent-host interactions and pathogenesis. Edited by Bradley R, Savey M, Marchant B. Sub-acute Spongiform Encephalopathies: Proceedings of the CEC Agricultural Research Programme Seminar 1990 Dordrecht: Kluwer Academic Publishers.; pp In 137–149
    [Google Scholar]
  11. Kitamoto T, Mohri S, Tateishi J. 1989; Organ Distribution of Proteinase-resistant Prion Protein in Humans and Mice with Creutzfeldt-Jakob Disease. Journal of General Virology 70:3371–3379
    [Google Scholar]
  12. Kretzschmar HA, Kufer P, Riethmuller G, DeArmond S, Prusiner SB, Schiffer D. 1992; Prion protein mutation at codon 102 in an Italian family with Gerstmann-Straussler-Scheinker syndrome. Neurology 42:809–809
    [Google Scholar]
  13. Laemmli UK. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  14. Lowenstein DH, Butler DA, Westaway D, Mckinley MP, Dearmond SJ, Prusiner SB. 1990; Three hamster species with different scrapie incubation times and neuropathological features encode distinct prion proteins.. Molecular and Cellular Biology 10:1153–1163
    [Google Scholar]
  15. McKinley MP, Bolton DC, Prusiner SB. 1983; A protease resistant protein is a structural component of the scrapie prion.. Cell 35:57–62
    [Google Scholar]
  16. Oesch B, Westaway D, Prusiner SB. 1991; Priori protein genes: evolutionary and functional aspects.. Current Topics in Microbiology and Immunology 172:109–124
    [Google Scholar]
  17. Prusiner SB, Scott M, Foster D, Foster D, Pan KM, Groth D, Mirenda C, Torchia M, Yang SL, Serban D, Carson GA, Hoppe PC, Westaway D, DeArmond SJ. 1990; Transgenetic studies implicate interactions between homologous PrP isoforms in scrapie prion replication.. Cell 63:673–686
    [Google Scholar]
  18. Scott M, Foster D, Mirenda C, Serban D, Coufal F, Wälchli M, Torchia M, Groth D, Carlson G, DeArmond SJ, Westaway D, Prusiner SB. 1989; Transgenic mice expressing hamster prion protein produce species-specific scrapie infectivity and amyloid plaques. Cell 59:847–857
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-7-1451
Loading
/content/journal/jgv/10.1099/0022-1317-74-7-1451
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error