1887

Abstract

Cattle infected with bovine viral diarrhoea virus (BVDV) often develop a lifelong persistent infection (PI). During this PI, BVDV infects many cell types including peripheral blood mononuclear cells (PBMNC). To define the lymphoid cell populations in which BVDV persists PBMNC subpopulations were separated using monoclonal antibodies to cell surface markers. Separated cells were analysed by a sensitive PCR assay for BVDV, in conjunction with flow cytometry to identify antigen-containing cells and with viral infectivity assays. The results indicate that BVDV establishes a productive PI in monocytes and T cells bearing the marker BoCD4, BoCD8 or gamma-delta T cell receptor. BVDV was not detected in B cells as a productive nor a latent infection.

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1993-05-01
2022-10-04
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References

  1. Allan G. M., McNulty M. S., Bryson D., Mackie D., Plamen M. 1989; Demonstration of bovine viral diarrhoea virus antigen in formalin fixed, paraffin embedded tissue using a streptavidin/biotin technique. Research in Veterinary Science 46:416–418
    [Google Scholar]
  2. Atluru D., Notowidjojo W., Johnson D. W., Muscoplat C. C. 1979; Suppression of in vitro immunoglobulin biosynthesis in bovine spleen cells by bovine viral diarrhea virus. Clinical Immunology and Immunopathology 13:254–260
    [Google Scholar]
  3. Atluru D., Xue W., Polam S., Atluru S., Blecha F., Minocha H. C. 1990; In vitro interactions of cytokines and bovine viral diarrhea virus in phytohemagglutinin stimulated bovine mononuclear cells. Veterinary Immunology and Immunopathology 25:47–59
    [Google Scholar]
  4. Bezek D. M., Baker J. C., Kaneene J. B. 1988; Immunofluorescence of bovine virus diarrhea viral antigen in white blood cells from experimentally infected immunocompetent calves. Canadian Journal of Veterinary Research 52:288–290
    [Google Scholar]
  5. Bielefeldt Ohmann H. 1983; Pathogenesis of bovine viral diarrhoea mucosal disease: distribution and significance of BVDV antigen in diseased calves. Research in Veterinary Science 34:5–10
    [Google Scholar]
  6. Bielefeldt Ohmann H. 1988; BVD virus antigens in tissues of persistently viraemic, clinically normal cattle: implications for the pathogenesis of clinically fatal disease. Acta veterinaria scandinavica 29:77–84
    [Google Scholar]
  7. Bielefeldt Ohmann H., Ronsholt L., Bloch B. 1987; Demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle. Journal of General Virology 68:1971–1982
    [Google Scholar]
  8. Bielefeldt Ohmann H., Bloch B., Davis W. C., Askaa J. 1988; BVD virus infection in peripheral blood mononuclear cells from persistently viraemic calves studied by correlative immunoelectron microscopy. Zentralblatt fur Veterinärmedizin 35:477–492
    [Google Scholar]
  9. Bolin S. R., Ridpath J. F. 1990; Frequency of association of noncytopathic bovine viral diarrhea virus with bovine neutrophils and mononuclear leukocytes before and after treatment with trypsin. American Journal of Veterinary Research 51:1847–1851
    [Google Scholar]
  10. Bolin S. R., McClurkin A. W., Coria M. F. 1985a; Effects of bovine viral diarrhea virus on the percentages and absolute numbers of circulating B and T lymphocytes in cattle. American Journal of Veterinary Research 46:884–886
    [Google Scholar]
  11. Bolin S. R., McClurkin A. W., Cutlip R. C., Coria M. F. 1985b; Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. American Journal of Veterinary Research 46:573–576
    [Google Scholar]
  12. Bolin S. R., Sacks J. M., Crowder S. V. 1987; Frequency of association of noncytopathic bovine viral diarrhea virus with mononuclear leukocytes from persistently infected cattle. American Journal of Veterinary Research 48:1441–1445
    [Google Scholar]
  13. Brown G. B., Bolin S. R., Frank D. R., Roth J. A. 1991; Defective function of leukocytes from cattle persistently infected with bovine viral diarrhea virus and the influence of recombinant cytokines. American Journal of Veterinary Research 52:381–387
    [Google Scholar]
  14. Brownlie J., Clarke M. C., Howard C. J. 1984; Experimental production of fatal mucosal disease in cattle. Veterinary Record 114:535–536
    [Google Scholar]
  15. Collett M., Anderson D. K., Retzel E. 1988; Comparisons of the pestivirus bovine diarrhoea virus with members of the Flaviviridae. Journal of General Virology 69:2637–2643
    [Google Scholar]
  16. Collett M. S., Moennio V., Horzinek M. C. 1989; Recent advances in pestivirus research. Journal of General Virology 70:253–266
    [Google Scholar]
  17. Corapi W. V., French T. W., Dubovi E. J. 1989; Severe thrombocytopenia in young calves experimentally infected with noncytopathic bovine viral diarrhea virus. Journal of Virology 63:3934–3943
    [Google Scholar]
  18. Donis R. O., Corapi W., Dubovi E. J. 1988; Neutralizing monoclonal antibodies to bovine diarrhoea virus bind to the 56K to 58K glycoprotein. Journal of General Virology 69:77–86
    [Google Scholar]
  19. Edwards S., Wood L., Hewitt T. C., Drew T. W. 1986; Evidence for an immunocompromising effect of bovine pestivirus on bovid herpesvirus I vaccination. Veterinary Research Communications 10:297–302
    [Google Scholar]
  20. Ellis J. A., Davis W. C., Belden E. L., Pratt D. L. 1988; Flow cytofluorimetric analysis of lymphocyte subset alterations in cattle infected with bovine viral diarrhea virus. Veterinary Pathology 25:231–236
    [Google Scholar]
  21. Gillespie J. H., Baker J. A., McEntee K. A. 1960; A cytopathogenic strain of virus diarrhea virus. Cornell Veterinarian 50:73–79
    [Google Scholar]
  22. Hein W. R., Mackay C. R. 1991; Prominence of gamma delta cells in the ruminant immune system. Immunology Today 12:29–34
    [Google Scholar]
  23. Horzinek M. C. 1991; Perspectives in pestivirus classification. Archives of Virology supplement 3:1–5
    [Google Scholar]
  24. Lee K. M., Gillespie J. H. 1957; Propagation of virus diarrhea of cattle in tissue culture. American Journal of Veterinary Research 18:952–953
    [Google Scholar]
  25. Lewis G. K., Kamin R. 1980 Separation of T and B cells using plastic surfaces coated with anti-immunoglobulin antibodies (“panning”). In Selected Methods in Cellular Immunology pp. 227–234 Edited by Mishell B., Shiigi S. New York: W. H. Freeman and Co;
    [Google Scholar]
  26. Lopez O. J., Osorio F. A., Done R. O. 1991; Rapid detection of bovine diarrhea virus by polymerase chain reaction. Journal of Clinical Microbiology 29:578–582
    [Google Scholar]
  27. McClurkin A. W., Littledicike E. T., Cutlip R. C., Frank G. H., Coria M. F., Bolin S. R. 1984; Production of cattle immunotolerant to bovine viral diarrhea virus. Canadian Journal of Comparative Medicine 48:156–161
    [Google Scholar]
  28. Moennig V. 1990; Pestivirus: a review. Veterinary Microbiology 23:35–54
    [Google Scholar]
  29. Reggiardo C., Kaeberle M. L. 1981; Detection of bacteremia in cattle inoculated with bovine viral diarrhea virus. American Journal of Veterinary Research 42:218–221
    [Google Scholar]
  30. Roth J. A., Kaeberle M. L. 1983; Suppression of neutrophil and lymphocyte function induced by a vaccinal strain of bovine viral diarrhea virus with and without the administration of ATCH. American Journal of Veterinary Research 44:2366–2372
    [Google Scholar]
  31. Roth J. A., Bolin S. R., Frank D. E. 1986; Lymphocyte blastogenesis and neutrophil function in cattle persistently infected with bovine diarrhea virus. American Journal of Veterinary Research 47:1139–1141
    [Google Scholar]
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