1887

Abstract

Cultured hepatoma cells (HepG2) were cotransfected with two different plasmids carrying a head-to-tail dimer of recombinant hepatitis B virus (HBV) DNA cloned from deletion mutants isolated from the circulation of persistently infected hosts. They were tested for the secretion of viral particles with mutant genome encapsidation. A recombinant plasmid defective in the S gene and one defective in both the C and P genes complemented in trans for the production of viral particles. Mutant genomes from either of the recombinants were encapsidated. Similarly, a recombinant defective in the C gene and another defective in the P gene trans-complemented for the production of viral particles containing mutant genomes. A hepatoma cell line with integrated HBV DNA sequences defective in the C and P genes (PLC/PRF/5) when transfected with a recombinant defective in the S gene produced viral particles with the HBV genome from the transfecting recombinants. These results confirm the expected trans-complementation among the S, C and P genes of HBV, when either episomal or integrated into chromosomes, for the maintenance of defective HBV mutants in persistently infected hosts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-3-407
1993-03-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/3/JV0740030407.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-3-407&mimeType=html&fmt=ahah

References

  1. Aden D. P., Fogel A., Plotkin S., Damjanov I., Knowles B. B. 1979; Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature, London 282:615–616
    [Google Scholar]
  2. Alexander J. J., Bey E. M., Geddes E. W., Lecatsas G. 1976; Establishment of a continuously growing cell line from primary carcinoma of the liver. South African Medical Journal 50:2124–2128
    [Google Scholar]
  3. Bartenschlager R., Junker-Niepmann M., Schaller H. 1990; The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. Journal of Virology 64:5324–5332
    [Google Scholar]
  4. Chang L. J., Pryciak P., Ganem D., Varmus H. E. 1989; Biosynthesis of the reverse transcriptase of hepatitis B viruses involves de novo translational initiation not ribosomal frameshifting. Nature, London 337:364–368
    [Google Scholar]
  5. Gerken G., Kremsdorf D., Capel F., Petit M. A., Dauguet C., Manns M. P., Meyer zum Büschenfelde K.-H., Brechot C. 1991; Hepatitis B defective virus with rearrangements in the preS gene during chronic HBV infection. Virology 183:555–565
    [Google Scholar]
  6. Hirsch R. C., Lavine J. E., Chang L., Varmus H. E., Ganem D. 1990; Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature, London 344:552–555
    [Google Scholar]
  7. Horwich A. L., Furtak K., Pugh J., Summers J. 1990; Synthesis of hepadnavirus particles that contain replication-defective duck hepatitis B virus genomes in cultured HuH7 cells. Journal of Virology 64:642–650
    [Google Scholar]
  8. Huang A. S., Baltimore D. 1977 Defective interfering animal viruses. In Comprehensive Virology vol 10 pp. 73–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  9. Imai M., Nomura M., Gotanda T., Sano T., Tachibana K., Miyamoto H., Takahashi K., Toyama S., Miyakawa Y., Mayumi M. 1982; Demonstration of two distinct antigenic determinants on hepatitis Be antigen by monoclonal antibodies. Journal of Immunology 128:69–72
    [Google Scholar]
  10. Imai M., Hoshi Y., Okamoto H., Matsui T., Tsurimoto T., Matsubara K., Miyakawa Y., Mayumi M. 1987; Free and integrated forms of hepatitis B virus DNA in human hepatocellular carcinoma cells (PLC/342) propagated in nude mice. Journal of Virology 61:3555–3560
    [Google Scholar]
  11. Jean-Jean O., Levrero M., Will H., Perricaudet M., Rossignol J.-M. 1989; Expression mechanism of the hepatitis B virus (HBV) C gene and biosynthesis of HBe antigen. Virology 170:99–106
    [Google Scholar]
  12. Junker-Niepmann M., Bartenschlager R., Schaller H. 1990; A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO Journal 9:3389–3396
    [Google Scholar]
  13. Matsui T., Takano M., Miyamoto K., Itoh Y., Yoshizawa H., Koike M., Mochizuki T., Tanaka E., Okamoto H., Imai M., Mishiro S., Miyakawa Y., Mayumi M. 1986; Nude mice bearing human primary hepatocellular carinoma that produces hepatitis B surface, core, and e antigens, as well as deoxyribonucleic acid polymerase. Gastroenterology 90:135–142
    [Google Scholar]
  14. Miller R. H., Girones R., Cote P. J., Hornbuckle W. E., Chestnut T., Baldwin B. H., Korba B. E., Tennant B. C., Gerin J. L., Purcell R. H. 1990; Evidence against a requisite role for defective virus in the establishment of persistent hepadnavirus infections. Proceedings of the National Academy of Sciences, U.S.A. 87:9329–9332
    [Google Scholar]
  15. Okamoto H., Tsuda F., Mayumi M. 1987a; Defective mutants of hepatitis B virus in the circulation of symptom-free carriers. Japanese Journal of Experimental Medicine 57:217–221
    [Google Scholar]
  16. Okamoto H., Imai M., Kametani M., Nakamura T., Mayumi M. 1987b; Genomic heterogeneity of hepatitis B virus in a 54-year-old woman who contracted the infection through matemo-fetal transmission. Japanese Journal of Experimental Medicine 57:231–236
    [Google Scholar]
  17. Okamoto H., Imai M., Tsuda F., Tanaka T., Miyakawa Y., Mayumi M. 1987c; Point mutation in the S gene of hepatitis B virus for a d/y or w/r subtypic change in two blood donors carrying a surface antigen of compound subtype adyr or adwr. Journal of Virology 61:3030–3034
    [Google Scholar]
  18. Okamoto H., Omi S., Wang Y., Imai M., Mayumi M. 1990a; Traus-complementation of the C gene of human and the P gene of woodchuck hepadnaviruses. Journal of General Virology 71:959–963
    [Google Scholar]
  19. Okamoto H., Yotsumoto S., Akahane Y., Yamanaka T., Miyazaki Y., Sugai Y., Tsuda F., Tanaka T., Miyakawa Y., Mayumi M. 1990b; Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen. Journal of Virology 64:1298–1303
    [Google Scholar]
  20. Randziwill G., Tucker W., Schaller H. 1990; Mutational analysis of the hepatitis B virus P gene product : domain structure and RNase H activity. Journal of Virology 64:613–620
    [Google Scholar]
  21. Schlicht H. J., Randziwill G., Schaller H. 1989; Synthesis and encapsidation of duck hepatitis B virus reverse transcriptase do not require formation of core-polymerase fusion proteins. Cell 56:85–92
    [Google Scholar]
  22. Takahashi K., Machida A., Funatsu G., Nomura M., Usuda S., Aoyagi S., Tachibana K., Miyamoto H., Imai M., Nakamura T., Miyakawa Y., Mayumi M. 1983; Immunochemical structure of hepatitis B e antigen in the serum. Journal of Immunology 130:2903–2907
    [Google Scholar]
  23. Takai E., Machida A., Ohnuma H., Miyamoto H., Tanaka T., Baba K., Tsuda F., Usuda S., Nakamura T., Miyakawa Y., Mayumi M. 1986; A solid-phase enzyme immunoassay for the determination of IgM and IgG antibodies against translation products of pre-Sl and pre-S2 regions of hepatitis B virus. Journal of Immunological Methods 95:23–30
    [Google Scholar]
  24. Tran A., Kremsdorf D., Capel F., Housset C., Dauguet C., Petit M.-A., Brechot C. 1991; Emergence of and takeover by hepatitis B virus (HBV) with rearrangements in the pre-S/S and pre-C/C genes during chronic HBV infection. Journal of Virology 65:3566–3574
    [Google Scholar]
  25. Usuda S., Tsuda F., Gotanda T., Tachibana K., Nomura M., Okamoto H., Imai M., Nakamura T., Miyakawa Y., Mayumi M. 1986; A solid-phase enzyme immunoassay for the common and subtypic determinants of hepatitis B surface antigen with monoclonal antibodies. Journal of Immunological Methods 87:203–210
    [Google Scholar]
  26. Wakita T., Kakumu S., Shibata M., Yoshioka K., Ito Y., Shinagawa T., Ishikawa T., Takayanagi M., Morishima T. 1991; Detection of pre-C and core region mutants of hepatitis B virus in chronic hepatitis B virus carriers. Journal of Clinical Investigation 88:1793–1801
    [Google Scholar]
  27. Will H., Reiser W., Weiner T., Pfaff E., Büscher M., Sprengel R., Cattaneo R., Schaller H. 1987; Replication strategy of human hepatitis B virus. Journal of Virology 61:904–911
    [Google Scholar]
  28. Ziemer M., García P., Shaul Y., Rutter W. J. 1985; Sequence of hepatitis B virus DNA incorporated into the genome of a human hepatoma cell line. Journal of Virology 53:885–892
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-74-3-407
Loading
/content/journal/jgv/10.1099/0022-1317-74-3-407
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error