1887

Abstract

The nucleotide sequences of a short fragment of the envelope protein gene encoding amino acids 25 to 89 of 27 dengue 3 viruses were determined by direct sequencing of PCR-amplified products, and the viruses were compared regarding their time of isolation and geographic distribution. Four distinct genotypic groups were discerned at 6% divergence between nucleotide sequences. The first group contained is olates from the South Pacific (1988 to 1992), Singapore (1973) and Indonesia (1973 to 1991). The second group comprised viruses from Asia (1956 to 1989) including the reference strain H-87. The third was composed of one isolate from Thailand (1971), and the fourth included the early strains from French Polynesia (1964 to 1969) and from Puerto Rico (1963). Furthermore, the difference between early and recent strains from the South Pacific was as high as 12.3%. This observation suggests that the recent epidemics in the South Pacific were probably the consequence of the spread of a new variant that emerged from New Caledonia. However, relatedness between nucleotide sequence and disease severity, or between strains from epidemics with mild disease (New Caledonia) and strains from epidemics with severe disease (French Polynesia) could not be demonstrated.

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1993-12-01
2022-09-28
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References

  1. Centers for Disease Control 1988; Update on dengue 2 epidemic in Palau. Dengue Surveillance Summary Newsletter 54:1–3
    [Google Scholar]
  2. Chungue E., Spiegel A., Roux J. F., Laudon F., Cardines R. 1990; Dengue 3 in French Polynesia: preliminary data. Medical Journal of Australia 152:557–558
    [Google Scholar]
  3. Chungue E., Burucoa C., Boutin J. P., Philippon G., Laudon F., Plichart R., Barbazan P., Cardines R., Roux J. F. 1992; Dengue 1 epidemic in French Polynesia, 1988–1989: surveillance and clinical, epidemiological, virological and serological findings in 1752 documented clinical cases. Transactions of the Royal Society of Tropical Medicine and Hygiene 86:193–197
    [Google Scholar]
  4. Chungue E., Roche C., Lefevre M. F., Barbazan P., Chanteau S. 1993; Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction. Journal of Medical Virology 40:142–145
    [Google Scholar]
  5. Deubel V., Laille M., Hugnot J. P., Chungue E., Guesdon J. L., Drouet M. T., Bassot S., Chevrier D. 1990; Identification of dengue sequences by genomic amplification: rapid diagnosis of dengue virus serotypes in peripheral blood. Journal of Virological Methods 30:41–54
    [Google Scholar]
  6. Deubel V., Nogueira R., Drouet M. T., Zeller H., Revnes J. P., Ha D. Q. 1993; Direct sequencing of genomic cDNA fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses. Archives of Virology 129:197–210
    [Google Scholar]
  7. Higgins D. G., Sharp P. M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151–153
    [Google Scholar]
  8. Keohavong P., Thilly W. G. 1989; Fidelity of DNA polymerases in DNA amplification. Proceedings of the National Academy of Sciences, U,. S,. A 86:9253–9257
    [Google Scholar]
  9. Lewis J. G., Chang G.-J., Lanciotti R. S., Trent D. W. 1992; Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations. Journal of Virological Methods 38:11–24
    [Google Scholar]
  10. Megret F., Hugnot J. P., Falconar A., Gentry M. K., Morens D. M., Murray J. M., Schlesinger J. J., Wright P. J., Young P., Van Regenmortel M. H. V., Deubel V. 1992; Use of recombinant fusion proteins and monoclonal antibodies to define linear and discontinuous antigenic sites on the dengue virus envelope glycoprotein. Virology 187:480–491
    [Google Scholar]
  11. Osatomi K., Sumiyoshi H. 1990; Complete nucleotide sequence of dengue type 3 virus genome RNA. Virology 176:643–647
    [Google Scholar]
  12. Rico-Hesse R. 1990; Molecular evolution of dengue serotype 1 and 2 in nature. Virology 174:479–493
    [Google Scholar]
  13. Rosen L. 1977; ‘The emperor’s new clothes’ revisited, or reflections on the pathogenesis of dengue hemorrhagic fever. American Journal of Tropical Medicine and Hygiene 26:337–343
    [Google Scholar]
  14. Russell P. K., McCown J. M. 1972; Comparison of dengue-2 and dengue-3 virus strains by neutralization tests and identification of a subtype of dengue-3. American Journal of Tropical Medicine and Hygiene 21:97–99
    [Google Scholar]
  15. Tukuitonga C. F., Maguire T. 1988; An epidemic of type 3 dengue on Niue Island. New Zealand Medical Journal 101:500–502
    [Google Scholar]
  16. Walker P. J., Henchal E. A., Blok J., Repik P. M., Henchal L. S., Burke D. S., Robbins S. J., Gorman B. M. 1988; Variation in dengue type 2 viruses isolated in Bangkok during 1980. Journal of General Virology 69:591–602
    [Google Scholar]
  17. WHO 1986; Dengue and dengue haemorrhagic fever in the Western Pacific. Weekly Epidemiological Record 61:206
    [Google Scholar]
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