1887

Abstract

The aim of this study was to define the helper T cell epitopes on the haemagglutinin (H) of measles virus (MV) in BALB/c (H-2) and TO (H-2) mice. A panel of 55 synthetic peptides (15-mers, overlapping by five amino acids) representing 92.2% of the H protein were synthesized and tested for immunogenicity and ability to stimulate MV-primed lymphocytes . The results obtained show that mouse lymphocytes respond to defined regions of the H protein which differ according to mouse strain. Virus-primed lymphocytes from BALB/c mice responded to peptides 7, 38, 39 and 44 whereas lymphocytes from virus-primed TO mice responded only to peptide 39. When mice of both strains were immunized with the peptides, a number of peptides induced proliferative responses, showing that the T cell repertoire for epitopes on the H protein is broader than that following immunization with virus. In BALB/c mice, lymphocytes primed to peptides 37, 39, 40, 42 and 43 responded to MV and in TO mice, lymphocytes primed to peptides 14, 32, 39, 40 and 49 responded to the virus. Thus in both strains of mice peptide 39 behaved as a dominant T cell epitope following immunization with virus or peptides. When the results obtained experimentally were compared with sequences predicted to be T cell epitopes by a number of algorithms, the concordance was limited.

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1993-12-01
2022-01-19
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References

  1. Adorini L., Nagy Z. A. 1990; Peptide competition for antigen presentation. Immunology Today 11:12–24
    [Google Scholar]
  2. Adorini L., Appella E., Doria G., Nagy Z. A. 1989; Mechanisms influencing the immunodominance of T-cell determinants. Journal of Experimental Medicine 168:2091–2104
    [Google Scholar]
  3. Alkhatib G., Briedis D. J. 1986; The predicted primary structure of the measles virus hemagglutinin. Virology 150:479–184
    [Google Scholar]
  4. Babbitt B. P., Allen P. M., Matsueda G., Haber H., Unanue E. R. 1985; Binding of immunogenic peptides to la histocompatibility molecules. Nature, London 317:359–361
    [Google Scholar]
  5. Berzofsky J. A. 1987; Ir genes: antigen-specific gene regulation of the immune response. In The Antigens pp 1–146 Edited by Sela M. New York: Academic Press;
    [Google Scholar]
  6. Berzofsky J. A., Cease K. B., Cornette J. L., Spouge J. L., Margalit H., DeLisi C. 1987; Protein antigen structures recognized by T-cells: potential application to vaccine design. Immunological Reviews 98:9–52
    [Google Scholar]
  7. Blackman M., Kappler J., Marrack P. 1990; The role of the T-cell receptor in positive and negative selection of developing T-cells. Science 248:1335–1341
    [Google Scholar]
  8. Brett S. J., Cease K. B., Berzofsky J. A. 1988; Influence of antigen processing on the expression of the T-cell repertoire: evidence for MHC-specific hindering structures on the products of processing. Journal of Experimental Medicine 168:357–373
    [Google Scholar]
  9. Buus S., Werdelin O. 1986; A group specific inhibitor of lysosomal cysteine proteinases selectively inhibits both proteolytic degradation and presentation of the antigen DNP-poly-L-lysine by guinea pig accessory cells to T cells. Journal of Immunology 136:452–158
    [Google Scholar]
  10. Buus S., Sette A., Colon S. M., Miles C., Grey H. M. 1987; The relation between major histocompatibility complex (MHC) restriction and the capacity of la to bind immunogenic peptides. Science 235:1353–1358
    [Google Scholar]
  11. Choppin P. W., Richardson C. D., Merz D. C., Hall W. W., Scheid A. 1981; The functions and inhibition of the membrane glycoproteins of paramyxoviruses and myxoviruses and the role of the measles virus M protein in subacute sclerosing panencephalitis. Journal of Infectious Diseases 143:352–368
    [Google Scholar]
  12. Drillien R., Spehner D., Kirn A., Giraudon P., Buckland R., Wild F., Lecocq J. P. 1988; Protection of mice from fatal measles encephalitis by vaccination with vaccinia virus recombinants encoding either the hemagglutinin or the fusion protein. Proceedings of the National Academy of Sciences, U,. S,. A 85:1252–1256
    [Google Scholar]
  13. Eisenlohr L. C., Gerhard W., Hackett C. J. 1988; Individual class II restricted antigenic determinants of the same protein exhibit distinct kinetics of appearance and persistence on antigen presenting cells. Journal of Immunology 141:2581–2584
    [Google Scholar]
  14. Gammon G., Shastri N., Cogswell J., Wilbur S., Sadegh-Nasseri S., Kryzych U., Miller A., Sercarz E. 1987; The choice of T cell epitopes utilised on a protein antigen depends on factors distant from as well as the determinant site. Immunological Reviews 98:53–73
    [Google Scholar]
  15. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–143
    [Google Scholar]
  16. Makela M. J., Smith R. H., Lund G. A., Salmi A. A. 1989; T-cell recognition of measles virus haemagglutinin studied in a mouse model. Scandinavian Journal of Immunology 29:597–607
    [Google Scholar]
  17. Malvoisin E., Wild F. 1990; Contribution of measles virus fusion protein in protective immunity: anti-F monoclonal antibodies neutralize virus infectivity and protect mice against challenge. Journal of Virology 64:5160–5162
    [Google Scholar]
  18. Margalit H., Spouge J. L., Cornett J. L., Cease K. B., DeLisi C., Berzofsky J. A. 1987; Prediction of immunodominant helper T cell antigenic sites from the primary sequence. Journal of Immunology 138:2213–2229
    [Google Scholar]
  19. Milich D. R. 1989; Synthetic T and B cell recognition sites: implications for vaccine development. Advances in Immunology 45:195–264
    [Google Scholar]
  20. Richardson C., Hull D., Greer P., Hasel K., Berkovich A., England G., Bellini W., Rima B., Lazzarini R. 1986; The nucleotide sequence of the mRNA encoding the fusion protein of measles virus (Edmonston strain): a comparison of fusion proteins from several different paramyxoviruses. Virology 155:508–523
    [Google Scholar]
  21. Rothbard J., Taylor W. 1988; A sequence pattern common to T cell epitopes. EMBO Journal 7:93–98
    [Google Scholar]
  22. Sette A., Buus S., Appella E., Smith J. A., Chesnut R., Miles G., Colon S. M., Grey H. M. 1989; Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysis. Proceedings of the National Academy of Sciences, U,. S,. A 86:3296–3301
    [Google Scholar]
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