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Two new cell lines developed from chinook salmon with plasmacytoid leukaemia have been found to be producing a virus. The virus has been identified as a retrovirus based on: type of c.p.e. induced in culture; morphology and density of the particle; presence of Mn2+-dependent, poly(rA)-directed reverse transcriptase activity which was associated with a density of 116 to 118 g/ml in sucrose; electrophoretic pattern of the polypeptides from purified virions; elevated [3H]UTP labelling of RNA in the cell cultures occurring at a density of 116 to 118 g/ml in sucrose. This report describes the first isolation of a retrovirus from a salmonid cell line.
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