1887

Abstract

Two new cell lines developed from chinook salmon with plasmacytoid leukaemia have been found to be producing a virus. The virus has been identified as a retrovirus based on: type of c.p.e. induced in culture; morphology and density of the particle; presence of Mn-dependent, poly(rA)-directed reverse transcriptase activity which was associated with a density of 116 to 118 g/ml in sucrose; electrophoretic pattern of the polypeptides from purified virions; elevated [H]UTP labelling of RNA in the cell cultures occurring at a density of 116 to 118 g/ml in sucrose. This report describes the first isolation of a retrovirus from a salmonid cell line.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-10-2299
1993-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/10/JV0740102299.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-10-2299&mimeType=html&fmt=ahah

References

  1. Anders K., Hilger I., Moller H. 1991; Lentivirus-like particles in connective tissue tumours of fish from German coastal waters. Diseases of Aquatic Organisms 11:151–154
    [Google Scholar]
  2. Burny A., Bex F., Chantrenne H., Cleuter Y., Dekegek D., Ghysdael J., Kettmann R., Leclercq M., Leunen J., Mam-merickx M., Portetelle D. 1978; Bovine leukemia virus involvement in enzootic bovine leukosis. Advances in Cancer Research 28:251–311
    [Google Scholar]
  3. Dahlberg J. E. 1988; An overview of retrovirus replication and classification. Advances in Veterinary Science and Comparative Medicine 32:1–16
    [Google Scholar]
  4. Duncan I. B. 1978; Evidence for an oncovirus in swimbladder fibrosarcoma of Atlantic salmon Salmo salar L. Journal of Fish Diseases 1:127–131
    [Google Scholar]
  5. Eaton W. D., Kent M. L. 1992; A retrovirus in chinook salmon (Oncorhynchus tshawytscha) with plasmacytoid leukemia and evidence for the etiology of the disease. Cancer Research 52:1–6
    [Google Scholar]
  6. Eaton W. D., Bagshaw J., Hulett J., Evans S. 1992; Isolation of a picorna-like virus from steelhead trout in Washington state. Journal of Aquatic Animal Health 4:90–96
    [Google Scholar]
  7. Frerichs G. N., Morgan D., Hart D., Skerrow C., Roberts R. J., Onions D. E. 1991; Spontaneously productive C-type retrovirus infection of fish cell lines. Journal of General Virology 72:2537–2539
    [Google Scholar]
  8. Kent M. L., Groff J. M., Traxler G. S., Zinkl J. G., Bagshaw J. W. 1990; Plasmacytoid leukemia in seawater reared chinook salmon, Oncorhynchus tshawytscha. Diseases of Aquatic Organisms 8:199–209
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Martineau D., Renshaw R., Williams J. R., Casey J. W., Bowser P. R. 1991; A large unintegrated retrovirus DNA species present in a dermal tumor of walleye Stizostedion vitreum. Diseases of Aquatic Organisms 10:153–158
    [Google Scholar]
  11. Martineau D., Bowser P. R., Renshaw R. R., Casey J. W. 1992; Molecular characterization of a unique retrovirus associated with a fish tumor. Journal of Virology 66:596–599
    [Google Scholar]
  12. Papas T. S., Dahlberg J. E., Sonstegard R. A. 1976; Type C virus in lymphosarcoma in northern pike (Esox lucius). Nature, London 261:506–508
    [Google Scholar]
  13. Stuart G. R., Dixon B., Pohajdak B. 1992; Isolation of a putative retrovirus pol gene fragment from trout. Comparative Biochemistry and Physiology 102B:137–142
    [Google Scholar]
  14. Walker R. 1969; A virus associated with epidermal hyperplasia in fish. National Cancer Institute Monograph 33:195–207
    [Google Scholar]
  15. Winqvist G., Lundberg O., Hellstroem B. 1968; Skin tumors of northern pike (Esox lucius L.) II. Viral particles in epidermal proliferations. Bulletin de VOjfice International des Epizooties 69:1023–1031
    [Google Scholar]
  16. Wolf K. 1979; Cold-blooded vertebrate cell and tissue culture. Methods in Enzymology 58:466–477
    [Google Scholar]
  17. Yamamoto T., Kelly R. K., Nielson O. 1983; Epidermal hyperplasias of northern pike (Esox lucius) associated with herpesvirus and C-type particles. Archives of Virology 79:255–272
    [Google Scholar]
  18. Yamamoto T., Kelly R. K., Nielson O. 1985; Morphological differentiation of virus-associated skin tumors of walleye (Stizo stedion vitreum vitreum). Fish Pathology 20:361–372
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-10-2299
Loading
/content/journal/jgv/10.1099/0022-1317-74-10-2299
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error