1887

Abstract

Two new cell lines developed from chinook salmon with plasmacytoid leukaemia have been found to be producing a virus. The virus has been identified as a retrovirus based on: type of c.p.e. induced in culture; morphology and density of the particle; presence of Mn-dependent, poly(rA)-directed reverse transcriptase activity which was associated with a density of 1·16 to 1·18 g/ml in sucrose; electrophoretic pattern of the polypeptides from purified virions; elevated [H]UTP labelling of RNA in the cell cultures occurring at a density of 1·16 to 1·18 g/ml in sucrose. This report describes the first isolation of a retrovirus from a salmonid cell line.

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/content/journal/jgv/10.1099/0022-1317-74-10-2299
1993-10-01
2020-01-24
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-10-2299
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