1887

Abstract

The right variable region of the genome of a pathogenic strain of African swine fever virus (ASFV), Malawi LIL20/1, has been sequenced and 15 open reading frames (ORFs) identified by computer analysis. Eight of these ORFs were found to be similar to previously described ASFV ORFs and three of these belong to two previously described multiple gene families (MGF), 360 and 110. Four of the remaining five ORFs belong to a novel MGF, designated MGF 100, and the last ORF encodes a protein that is similar to the virus structural protein, p22. Copies of MGF 110 and the gene coding for p22 have previously been characterized only at the left end of the ASFV genome. The organization of these genes suggests evolution by duplications, deletions and sequence transposition from one end of the genome to the other. Sequence comparisons of members of MGF 360 suggest that the Malawi LIL20/1 genome has undergone separate DNA rearrangements compared to the Ba71V genome. Lastly, one ORF was found to be similar to the myeloid differentiation primary response protein, MyD116 and to the herpes simplex virus neurovirulence-associated factor ICP34.5.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-10-2125
1993-10-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/10/JV0740102125.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-10-2125&mimeType=html&fmt=ahah

References

  1. Agüero M., Blasco R., Wilkinson P., Vinuela E. 1990 Analysis of naturally occurring deletion variants of African swine fever virus: multigene family 110 is not essential for infectivity or virulence in pigs.Virology 176:195–204
    [Google Scholar]
  2. Almazán F., Rodríguez J. M., Andrés G., Pérez R., Viñuela E., Rodriguez J. F. 1992; Transcriptional analysis of multigene family 110 of African swine fever virus. Journal of Virology 66:6655–6667
    [Google Scholar]
  3. Almazán F., Rodríguez J. M., Angulo A., Viñuela E., Rodriguez J. F. 1993; Transcriptional mapping of a late gene coding for the p12 attachment protein of African swine fever virus. Journal of Virology 67:553–556
    [Google Scholar]
  4. Almendral J. M., Almazán F., Blasco R., Viñuela E. 1990; Multigene families in African swine fever virus: family 110. Journal of Virology 64:2064–2072
    [Google Scholar]
  5. Blasco R., de la Vega R., Almazán F., Agüero H., Viñuela E. 1989; Genetic variations of African swine fever virus: variable regions near the ends of the viral DNA. Virology 173:251–257
    [Google Scholar]
  6. Camacho A., Viñuela E. 1991; Protein p22 of African swine fever virus: an early structural protein that is incorporated into the membrane of infected cells. Virology 181:251–257
    [Google Scholar]
  7. Casal I., Enjuanes L., Viñuela E. 1984; Porcine leukocyte cellular subsets sensitive to African swine fever virus in vitro. Journal of Virology 52:37–46
    [Google Scholar]
  8. Chou J., Roizman B. 1990; The herpes simplex virus 1 gene for ICP34.5, which maps in inverted repeats, is conserved in several limited-passage isolates but not in strain 17 syn+. Journal of Virology 64:1014–1020
    [Google Scholar]
  9. Chou J., Roizman B. 1992; The γ1 34.5 gene of herpes simplex virus 1 precludes neuroblastoma cells from triggering total shutoff of protein synthesis characteristics of programmed cell death in neuronal cells. Proceedings of the National Academy of Sciences, U,. S,. A. 89:3266–3270
    [Google Scholar]
  10. Collins J. F., Coulson A. F. W. 1987; Molecular sequence comparison and alignment. In Nucleic Acid and Protein Sequence Analysis:A Practical Approach pp 323–358 Edited by Bishop M., Rawlings C. Oxford: IRL Press;
    [Google Scholar]
  11. Costa J. V. 1990; African swine fever virus. In Molecular Biology of Iridoviruses pp 247–270 Edited by Darai G. Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  12. de la Vega I., Viñuela E., Blasco R. 1990; Genetic variation and multigene families in African swine fever virus. Virology 179:234–246
    [Google Scholar]
  13. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  14. Dixon L. K. 1988; Molecular cloning and restriction enzyme mapping of an African swine fever virus isolate from Malawi. Journal of General Virology 69:1683–1694
    [Google Scholar]
  15. Dixon L. K., Wilkinson P. J. 1988; Genetic diversity of African swine fever virus isolates from soft ticks (Ornithodoros moubata) inhabiting warthog burrows in Zambia. Journal of General Virology 69:2981–2993
    [Google Scholar]
  16. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  17. González A., Talavera A., Almendral J. M., Viñuela E. 1986; Hairpin loop structure of African swine fever virus DNA. Nucleic Acids Research 14:6835–6844
    [Google Scholar]
  18. González A., Calvo V., Almazan F., Almendral J. M., Ramírez J. C., de la Vega I., Blasco R., Viñuela E. 1990; Multigene families in African swine fever virus: family 360. Journal of Virology 64:2073–2081
    [Google Scholar]
  19. Hess W. R. 1981; African swine fever: a reassessment. Advances in Veterinary Science and Comparative Medicine 25:39–69
    [Google Scholar]
  20. Lord K. A., Hoffman-Liebermann B., Liebermann D. A. 1990; Sequence of MyD116 cDNA: a novel myeloid differentiation primary response gene induced by IL6. Nucleic Acids Research 18:2823
    [Google Scholar]
  21. McGeoch D. J., Barnett B. C. 1991; Neurovirulence factor. Nature, London 353:609
    [Google Scholar]
  22. Moyer R. W., Graves R. L. 1981; The mechanism of cytoplasmic orthopoxvirus DNA replication. Cell 27:391–101
    [Google Scholar]
  23. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, U,. S,. A 85:2444–2448
    [Google Scholar]
  24. Pickup D. J., Ink B. S., Parsons B. L., Hu W., Joklik W. K. 1984; Spontaneous deletions and duplications of sequences in the genome of cowpox virus. Proceedings of the National Academy of Sciences, U,. S,. A 81:6817–6821
    [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U,. S,. A 74:5463–5467
    [Google Scholar]
  26. Sogo J. M., Almendral J. M., Talavera A., Viñuela E. 1984; Terminal and internal inverted repetitions in African swine fever virus DNA. Virology 133:271–275
    [Google Scholar]
  27. Staden R. 1982; Automation of computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:473–4751
    [Google Scholar]
  28. Sumption K. J., Hutchings G. H., Wilkinson P. J., Dixon L. K. 1990; Variable regions on the genome of Malawi isolates of African swine fever virus. Journal of General Virology 71:2331–2340
    [Google Scholar]
  29. Sussman M. D., Lu Z., Kutish G., Alfonso C. L., Roberts P., Rock D. C. 1992; Identification of an African swine fever virus gene with similarity to a myeloid differentiation primary response gene and neurovirulence associated gene of herpes simplex virus. Journal of Virology 66:5586–5589
    [Google Scholar]
  30. Viñuela E. 1985; African swine fever virus. Current Topics in Microbiology and Immunology 116:151–170
    [Google Scholar]
  31. Walton G. A. 1964; The Ornithodorosmoubata” group of ticks in Africa. Control problems and implications. Journal of Medical Entomology 1:53–64
    [Google Scholar]
  32. Wilkinson P. J. 1984; The persistence of African swine fever in Africa and the Mediterranean. Preventive Veterinary Medicine 2:71–82
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-74-10-2125
Loading
/content/journal/jgv/10.1099/0022-1317-74-10-2125
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error