1887

Abstract

A recombinant baculovirus expressing the M2 protein from influenza A/Ann Arbor/6/60 (H2N2) virus (AA60 virus) was constructed. The expressed M2 protein was recognized by a monoclonal antibody specific for the M2 protein and comigrated with the M2 protein from cells infected with AA60 virus on SDS–polyacrylamide gels. Immunofluorescence studies indicated that the expressed M2 protein was present on the surface of (Sf9) cells infected with the recombinant baculovirus. Immunoassays using the expressed M2 protein were able to detect antibodies to the M2 protein in serum samples from humans and ferrets infected with influenza A viruses.

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1993-01-01
2024-11-12
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References

  1. Belshe RB, Hay AJ. 1989; Drug resistance and mechanisms of action on influenza A viruses. Journal of Respiratory Disease 10:S52–S61
    [Google Scholar]
  2. Belshe RB, Smith MH, Hall HB, Betts R, Hay AJ. 1988; Genetic basis of resistance to rimantadine emerging during treatment of influenza virus infection. Journal of Virology 62:1508–1512
    [Google Scholar]
  3. Ciampor F, Bayley PM, Nermut MV, Hirst EMA, Sugrue RJ, Hay AJ. 1992a; Evidence that the amantadine-induced, M2-mediated conversion of influenza A virus hemagglutinin to the low pH form occurs in an acidic trans Golgi compartment. Virology 188:14–24
    [Google Scholar]
  4. Ciampor F, Thompson CA, Grambas S, Hay AJ. 1992ft; Regulation of pH by the M2 protein of influenza A viruses. Virus Research 22:247–258
    [Google Scholar]
  5. Cox NJ, Kitame F, Klimov A, Koennecke I, Kendal AP. 1986; Comparative studies of wild-type and cold-mutations in all genes of the A/Ann Arbor/6/60 (H2N2) mutant vaccine donor strain. Microbial Pathogenesis 1:387–397
    [Google Scholar]
  6. Gotch F, McMichael A, Smith G, Moss B. 1987; Identification of viral molecules recognized by influenza-specific cytotoxic T lymphocytes. Journal of Experimental Medicine 165:408–416
    [Google Scholar]
  7. Hay AJ. 1989; The mechanism of action of amantadine and rimantadine against influenza viruses. In. In Concepts in Viral Pathogenesis III pp 561–567Edited by Notkins AL, Oldstone MBA. New York: Springer-Verlag;
    [Google Scholar]
  8. Hay AJ, Zambon MC, Wolstenholme AJ, Skehel JJ, Smith MH. 1986; Molecular basis of resistance of influenza A viruses to amantadine. Journal of Antimicrobial Chemotherapy (Supplement B) 18:19–29
    [Google Scholar]
  9. Holsinger LJ, Lamb RA. 1991; Influenza virus M2 membrane protein is a homotetramer stabilized by formation of disulfide bonds. Virology 183:32–43
    [Google Scholar]
  10. Ito T, Gorman OT, Kawaoka Y, Bean WJ, Webster RJ. 1991; Evolutionary analysis of the influenza A virus M gene with comparison of the Ml and M2 proteins. Journal of Virology 65:5491–5498
    [Google Scholar]
  11. Lamb RA, Choppin PW. 1981; Identification of a second protein (M2) encoded by RNA segment 7 of influenza virus. Virology 112:729–737
    [Google Scholar]
  12. Lamb RA, Lai CJ. 1982; Spliced and unspliced messenger RNAs synthesized from cloned influenza virus M DNA in an SV40 vector: expression of the influenza virus membrane protein (M1). Virology 113:237–356
    [Google Scholar]
  13. Lamb RA, Zebedee SL, Richardson CD. 1985; Influenza virus M2 protein is an integral membrane protein expressed on the infected cell surface. Cell 40:627–633
    [Google Scholar]
  14. Matsuura Y, Possee RD, Overton HA, Bishop DHL. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  15. Pinto LH, Holsinger LJ, Lamb RA. 1992; Influenza virus M2 protein has ion channel activity. Cell 69:517–528
    [Google Scholar]
  16. Rota PA, Black RA, De BK, Harmon MW, Kendal AP. 1990; Expression of influenza A and B virus nucleoprotein antigens in baculovirus. Journal of General Virology 71:1545–1554
    [Google Scholar]
  17. Sanger F, Nicklen S, Coulson AR. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  18. Skehel JJ, Hay AJ, Armstrong JA. 1977; On the mechanism of inhibition of influenza virus replication by amantadine hydrochloride. Journal of General Virology 38:97–110
    [Google Scholar]
  19. Steinhauer DA, Wharton SA, Skehel JJ, Wiley DC, Hay AJ. 1991; Amantadine selection of a mutant influenza virus containing an acid-stable hemagglutinin: evidence for virus-specific regulation of the pH of glycoprotein transport vesicles. Proceedings of the National Academy of Sciences, U.S.A. 88:11525–11529
    [Google Scholar]
  20. Sugrue RJ, Hay AJ. 1991; Structural characteristics of the M2 protein of influenza A viruses: evidence that it forms a tetrameric channel. Virology 180:617–627
    [Google Scholar]
  21. Sugrue RJ, Bahadur G, Zambon MC, Hall-Smith M, Douglas AR, Hay AJ. 1990; Specific structural alteration of the influenza haemagglutinin by amantadine. EMBO Journal 9:3469–3476
    [Google Scholar]
  22. Summers MD, Smith GE. 1986; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin1555
    [Google Scholar]
  23. Treanor JJ, Tierney EL, Zebedee SL, Lamb RA, Murphy BR. 1990; Passively transferred monoclonal antibody to the M2 protein inhibits influenza A virus replication in mice. Journal of Virology 64:1375–1377
    [Google Scholar]
  24. Veit M, Klenk H-D, Kendal A, Rott R. 1991; The M2 protein of influenza A virus is acylated. Journal of General Virology 72:1461–1465
    [Google Scholar]
  25. Zebedee SL, Lamb RA. 1988; Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions. Journal of Virology 62:2762–2772
    [Google Scholar]
  26. Zebedee SL, Richardson CD, Lamb RA. 1985; Characterization of the influenza virus M2 integral membrane protein and expression at the infected cell surface from cloned cDNA. Journal of Virology 56:502–511
    [Google Scholar]
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