1887

Abstract

The nucleotide sequences of the RNA-2s of two strains of sweet clover necrotic mosaic virus (SCNMV-38 and -59) have been determined. The RNA-2s of SCNMV-38 and -59 consist of 1446 and 1449 nucleotides, respectively, and both contain one major open reading frame (ORF) which potentially can encode polypeptides of 326 amino acid residues (about 36.5K), designated SC38P2 and SC59P2, respectively. The nucleotide sequences of SCNMV-38 and -59 RNA-2s show 93.2% similarity, and the amino acid sequences of SC38P2 and SC59P2 are 91.7% identical, although the identical nucleotides and amino acids are not distributed uniformly in RNA-2 and the encoded proteins. Two highly conserved regions (from positions 23 to 221 and 297 to 326) and a relatively divergent region (from positions 222 to 296) are found in the P2 proteins of these strains. A similar pattern is apparent on comparison of the nucleotide and deduced amino acid sequences of RNA-2 of these SCNMV strains with those of the Australian and Czechoslovakian isolates of red clover necrotic mosaic virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-9-2483
1992-09-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/9/JV0730092483.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-9-2483&mimeType=html&fmt=ahah

References

  1. Ahlquist P., Dasgupta R., Kaesberg P. 1981; Near identity of 3′ secondary structure in bromoviruses and cucumber mosaic virus. Cell 23:183–189
    [Google Scholar]
  2. Bujarski J. J., Ahlquist P., Hall T. C., Dreher T. W., Kaesberg P. 1986; Modulation of replication, aminoacylation and adenylation in vitro and infectivity in vivo of BMV RNAs containing deletions within the multifunctional 3′-end. EMBO Journal 5:1769–1774
    [Google Scholar]
  3. Chang S. H., Strauss E. G., Strauss J. H. 1989; Dideoxy sequencing of RNA using reverse transcriptase. Methods in Enzymology 180:121–130
    [Google Scholar]
  4. Dreher T. W., Bujarski J. J., Hall T. C. 1984; Mutant viral RNAs synthesized in vitro show altered aminoacylation and replicase template activities. Nature, London 311:171–175
    [Google Scholar]
  5. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and nomenclature of viruses. Fifth report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2:
    [Google Scholar]
  6. Hiruki C., Rao D. V., Chen M. H., Okuno T., Figueiredo G. 1984; Characterization of sweet clover necrotic mosaic virus. Phytopathology 74:482–486
    [Google Scholar]
  7. Kendall T. L., Lommel S. A. 1992; Nucleotide sequence of carnation ringspot dianthovirus RNA-2. Journal of General Virology 73:2479–2482
    [Google Scholar]
  8. Lommel S. A., Weston-Fina M., Xiong Z., Lomonossoff G. P. 1988; The nucleotide sequence and gene organization of red clover necrotic mosaic virus RNA-2. Nucleic Acids Research 16:8587–8602
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Okuno T., Hiruki C., Rao D. V., Figueiredo G. 1983; Genetic determinants distributed in two genomic RNAs of sweet clover necrotic mosaic, red clover necrotic mosaic and primary leaf necrotic viruses. Journal of General Virology 64:1907–1914
    [Google Scholar]
  11. Osman T. A. M., Buck K. W. 1991; Detection of the movement protein of red clover necrotic mosaic virus in a cell wall fraction from infected Nicotiana clevelandii plants. Journal of General Virology 72:2853–2856
    [Google Scholar]
  12. Osman T. A. M., Dodds S. M., Buck K. W. 1986; RNA 2 of red clover necrotic mosaic virus determines lesion morphology and systemic invasion in cowpea. Journal of General Virology 67:203–207
    [Google Scholar]
  13. Osman T. A. M., Ingles P. J., Miller S. J., Buck K. W. 1991a; A spontaneous red clover necrotic mosaic virus mutant with a truncated movement protein. Journal of General Virology 72:1793–1900
    [Google Scholar]
  14. Osman T. A. M., Miller S. J., Marriott A. C., Buck K. W. 1991b; Nucleotide sequence of RNA 2 of a Czechoslovakian isolate of red clover necrotic mosaic virus. Journal of General Virology 72:213–216
    [Google Scholar]
  15. Paje-Manalo L. L., Lommel S. A. 1989; Independent replication of red clover necrotic mosaic virus RNA-1 in electroporated host and nonhost Nicotiana species protoplasts. Phytopathology 79:457–461
    [Google Scholar]
  16. Pappu H. R., Hiruki C. 1988; Replication of sweet clover necrotic mosaic virus in cowpea protoplasts. Canadian Journal of Plant Pathology 10:110–115
    [Google Scholar]
  17. Pappu H. R., Hiruki C. 1989; Characterization of genetic reassortants of sweet clover necrotic mosaic virus by virion electrophoresis. Canadian Journal of Plant Pathology 11:247–250
    [Google Scholar]
  18. Pappu H. R., Hiruki C., Inouye N. 1988; A new serotype of sweet clover necrotic mosaic virus. Phytopathology 78:1343–1348
    [Google Scholar]
  19. Rao A. L. N., Hiruki C. 1987; Unilateral compatibility of genome segments from two distinct strains of red clover necrotic mosaic virus. Journal of General Virology 68:191–194
    [Google Scholar]
  20. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded phage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-9-2483
Loading
/content/journal/jgv/10.1099/0022-1317-73-9-2483
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error