1887

Abstract

The complete sequence of RNA segment 5 of the African horsesickness virus serotype 4 (AHSV-4) vaccine strain was determined from cDNA clones inserted into pBR322. The RNA is 1751 bp long ( 1.12 × 10) and contains an open reading frame encoding a protein of 548 amino acids ( 63 122) with a net charge of +0.5 at neutral pH. A comparison of the sequence of AHSV-4 segment 5 with that of segment 6 of bluetongue virus (BTV) serotypes 10 and 17 revealed 49.2% and 48.9% nucleotide similarity, respectively, and 31.4% amino acid similarity. However, AHSV-4 segment 5 has no significant similarity to BTV segment 5. In addition, Northern blot hybridization showed that full-length AHSV-4 segment 5 cDNA cross-hybridized with the corresponding genes of all serotypes of attenuated AHSV.

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1992-09-01
2022-01-19
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References

  1. Bremer C. W. 1976; A gel electrophoretic study of the protein and nucleic acid components of African horsesickness virus. Onder-stepoort Journal of Veterinary Research 43:193–200
    [Google Scholar]
  2. Bremer C. W., Huismans H., Van Dijk A. A. 1990; Characterization and cloning of the African horsesickness virus genome. Journal of General Virology 71:793–799
    [Google Scholar]
  3. Diaz-Ruiz J. R., Kaper J. M. 1978; Isolation of viral doublestranded RNAs using a LiCl fractionation procedure. Preparative Biochemistry 8:1–17
    [Google Scholar]
  4. French T. J., Inumaru S., Roy P. 1989; Expression of two related nonstructural proteins of bluetongue virus (BTV) type 10 in insect cells by a recombinant baculovirus: production of polyclonal ascitic fluid and characterization of the gene product in BTV-infected BHK cells. Journal of Virology 63:3270–3278
    [Google Scholar]
  5. Gould A. R., Pritchard L. I. 1988; The complete nucleotide sequence of the outer coat protein, VP5, of the Australian bluetongue virus (BTV) serotype 1 reveals conserved and non-conserved sequences. Virus Research 9:285–292
    [Google Scholar]
  6. Grubman M. J., Lewis S. A. 1992; Identification and characterization of the structural and nonstructural proteins of African horsesickness virus and determination of the genome coding assignments. Virology 186:444–451
    [Google Scholar]
  7. Holmes I. H. 1991; Classification and nomenclature of viruses. Archives of Virology, supplementum 2:188–189
    [Google Scholar]
  8. Huismans H. 1979; Protein synthesis in bluetongue virus-infected cells. Virology 92:385–396
    [Google Scholar]
  9. Huismans H., Cloete M. 1987; A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specified RNA. Virology 158:373–380
    [Google Scholar]
  10. Huismans H., Els H. J. 1979; Characterization of the tubules associated with the replication of three different orbiviruses. Virology 92:397–406
    [Google Scholar]
  11. Huismans H., Verwoerd D. W. 1973; Control of transcription during the expression of the bluetongue virus genome. Virology 52:81–88
    [Google Scholar]
  12. Lecatsas G. 1968; Electron microscopic study of the formation of bluetongue virus. Onderstepoort Journal of Veterinary Research 35:139–150
    [Google Scholar]
  13. Lee J., Roy P. 1987; Complete sequence of the NS1 gene (M6 RNA) of US bluetongue virus serotype 10. Nucleic Acids Research 15:7207
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Mertens P. P. C., Sangar D. V. 1985; Analysis of the terminal sequences of the genome segments of four orbiviruses. Virology 140:55–67
    [Google Scholar]
  16. Mertens P. P. C., BROWN F., Sangar D. V. 1984; Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135:207–217
    [Google Scholar]
  17. Oellermann R. A., Els H. J., Erasmus B. J. 1970; Characterization of African horsesickness virus. Archiv für die gesamte Virusforschung 29:163–174
    [Google Scholar]
  18. Purdy M., Petre J., Roy P. 1984; Cloning of the bluetongue virus L3 gene. Journal of Virology 51:754–759
    [Google Scholar]
  19. Rao C. D., Kiuchi A., Roy P. 1983; Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus. Journal of Virology 46:378–383
    [Google Scholar]
  20. Ritter D. G., Roy P. 1988; Genetic relationships of bluetongue virus serotypes isolated from different parts of the world. Virus Research 11:33–47
    [Google Scholar]
  21. Roy P., Hirasawa T., Fernandez M., Blinov V. M., Sanchez-Vixcain Rodrique J. M. 1991; The complete sequence of the group-specific antigen, VP7, of African horsesickness disease virus serotype 4 reveals a close relationship to bluetongue virus. Journal of General Virology 72:1237–1241
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  23. Sugiyama K., Bishop D. H. L., Roy P. 1982; Analyses of the genomes of bluetongue viruses recovered from different states of the United States and at different times. American Journal of Epidemiology 115:332–347
    [Google Scholar]
  24. Urakawa T., Roy P. 1988; Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of NS1 gene of bluetongue virus serotype 10. Journal of Virology 62:3919–3927
    [Google Scholar]
  25. Van Dijk A. A., Huismans H. 1988; In vitro transcription and translation of bluetongue virus mRNA. Journal of General Virology 69:573–581
    [Google Scholar]
  26. Van Staden V., Huismans H. 1991; A comparison of the genes which encode non-structural protein NS3 of different orbiviruses. Journal of General Virology 72:1073–1079
    [Google Scholar]
  27. Wade-Evans A. M., Pan Z. Q., Mertens P. P. C. 1988; Sequence analysis and in vitro expression of a cDNA clone of genome segment 5 from bluetongue virus, serotype 1 from South Africa. Virus Research 11:227–240
    [Google Scholar]
  28. Wang L., Doi R. H., Osburn B. I., Chuang R. Y. 1989; Complete sequence of the NS1 gene (S6 RNA) of US bluetongue virus serotype 17. Nucleic Acids Research 17:8002
    [Google Scholar]
  29. Wilson W. C., Fukusho A., Roy P. 1990; Diagnostic complementary DNA probes for genome segments 2 and 3 of epizootic hemorrhagic disease virus serotype 1. American Journal of Veterinary Research 51:855–860
    [Google Scholar]
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