1887

Abstract

Functional analysis of human papillomavirus type 16 E7 protein by complementation with adenovirus E1A mutants in baby rat kidney cells has shown that the retinoblastoma gene product (RB)-binding region of E7 can substitute in trans for that of E1A. An N-terminal E7 mutant was unable to complement an E1A mutant unable to bind p300, indicating that the two mutants were defective for functionally equivalent activities. E7 proteins with mutations within the RB-binding region were also unable to complement either the non-p300-binding E1A mutant or the N-terminal E7 mutant, suggesting that these mutations affect more than just RB binding.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-8-2135
1992-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/8/JV0730082135.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-8-2135&mimeType=html&fmt=ahah

References

  1. Banks L., Edmonds C., Vousden K. H. 1990; Ability of the HPV16 E7 protein to bind RB and induce DNA synthesis is not sufficient for efficient transforming activity in NIH3T3 cells. Oncogene 5:1383–1389
    [Google Scholar]
  2. Barbosa M. S., Edmonds C., Fisher C., Schiller J. T., Lowy D. R., Vousden K. H. 1990; The region of the HPV E7 oncoprotein homologous to adenovirus E1a and SV40 large T antigen contains separate domains for Rb binding and casein kinase II phosphorylation. EMBO Journal 9:153–160
    [Google Scholar]
  3. Chesters P. M., Vousden K. H., Edmonds C., McCance D. J. 1990; Analysis of human papillomavirus type 16 open reading frame E7 immortalizing function in rat embryo fibroblast cells. Journal of General Virology 71:449–453
    [Google Scholar]
  4. Crook T., Storey A., Almond A., Osborn K., Crawford L. 1988; Human papillomavirus type 16 cooperates with activated ras and fos oncogenes in the hormone dependent transformation of primary mouse cells. Proceedings of the National Academy of Sciences, U.S.A. 85:8820–8824
    [Google Scholar]
  5. DeCaprio J. A., Ludlow J. W., Figge J., Shew J.-Y., Huang C.-M., Lee W.-H., Marsillo E., Paucha E., Livingston D. M. 1988; SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene. Cell 54:275–283
    [Google Scholar]
  6. Dyson N., Howley P. M., Münger K., Harlow E. 1989; The human papillomavirus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243:934–936
    [Google Scholar]
  7. Edmonds C., Vousden K. H. 1989; A point mutational analysis of human papillomavirus type 16 E7 protein. Journal of Virology 63:2650–2656
    [Google Scholar]
  8. Egan C., Jelsma T. N., Rowe J. A., Bayley S. T., Ferguson B., Branton P. E. 1988; Mapping of the cellular protein-binding sites on the products of early-region 1A of human adenovirus type 5. Molecular and Cellular Biology 8:3955–3959
    [Google Scholar]
  9. Ewen M. E., Ludlow J. W., Marsillo E., DeCaprio J. A., Millikan R. C., Livingston D. M. 1989; An N-terminal transformation-governing sequence of SV40 large T antigen contributes to the binding of both pll0RB and a second cellular protein: p120. Cell 58:257–267
    [Google Scholar]
  10. Firzlaff J. M., Galloway D. A., Eisenman R. N., Luscher B. 1989; The E7 protein of human papillomavirus type 16 is phosphorylated by casein kinase II. New Biologist 1:44–53
    [Google Scholar]
  11. Gissmann L., Boshart M., Dürst M., Ikenberg H., Wagner D. 1984; Presence of human papillomavirus (HPV) DNA in genital tumours. Journal of Investigative Dermatology 83:265–288
    [Google Scholar]
  12. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
    [Google Scholar]
  13. Harlow E., Franza B. R. Jr, Schley C. 1985; Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products. Journal of Virology 55:533–546
    [Google Scholar]
  14. Harlow E., Whyte P., Franza B. R. Jr, Schley C. 1986; Association of adenovirus early region 1A protein with cellular polypeptides. Molecular and Cellular Biology 6:1570–1589
    [Google Scholar]
  15. Hawley-Nelson P., Vousden K. H., Hubbert N. L., Lowy D. R., Schiller J. T. 1989; HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. EMBO Journal 8:3905–3910
    [Google Scholar]
  16. Jelsma T. N., Howe J. A., Mymyrk J. S., Evelegh C. M., Cunniff N. F. A., Bayley S. T. 1988; Sequences in E1A proteins of human adenovirus 5 required for cell transformation, repression of a transcriptional enhancer, and induction of proliferating nuclear antigen. Virology 171:120–130
    [Google Scholar]
  17. Jewers R. J., Hildebrandt P., Ludlow J. W., Kell B., McCance D. J. 1992; Regions of human papillomavirus type 16 E7 oncoprotein required for immortalization of human keratinocytes. Journal of Virology 66:1329–1335
    [Google Scholar]
  18. Lane D. P., Crawford L. V. 1979; T antigen is bound to a host protein in SV40-transformed cells. Nature, London 278:261–263
    [Google Scholar]
  19. Moran E. 1988; A region of SV40 large T antigen can substitute fora transforming domain of adenovirus El A products. Nature, London 334:168–170
    [Google Scholar]
  20. Moran E., Zerler B. 1988; Interactions between cell growth regulating domains in the products of the adenovirus El A oncogene. Molecular and Cellular Biology 8:1755–1764
    [Google Scholar]
  21. Münger K., Phelps W. C., Bubb V., Howley P. M., Schlegel R. 1989; The E6 and E7 genes of the human papillomavirus type 16 together are sufficient for transformation of primary human keratinocytes. Journal of Virology 63:4417–4421
    [Google Scholar]
  22. Phelps W. C., Yee C. L., Münger K., Howley P. M. 1988; The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus El a. Cell 53:539–547
    [Google Scholar]
  23. Riley T. E. W., Follin A., Jones N. C., Jat P. S. 1990; Maintenance of cellular proliferation by adenovirus early region 1A in fibroblasts conditionally immortalised by using simian virus 40 large T antigen requires conserved region 1. Molecular and Cellular Biology 10:6664–6673
    [Google Scholar]
  24. Schneider J. F., Fisher F., Goding C. R., Jones N. C. 1987; Mutational analysis of the adenovirus E1a gene: the role of transcriptional regulation in transformation. EMBO Journal 6:2053–2060
    [Google Scholar]
  25. Shih C., Weinberg R. A. 1982; Isolation of a transforming sequence from a human carcinoma cell line. Cell 29:161–169
    [Google Scholar]
  26. Smotkin D., Wettstein F. O. 1987; Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein. Journal of Virology 61:1686–1689
    [Google Scholar]
  27. Southern P., Berg P. 1982; Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. Journal of Molecular and Applied Genetics 1:327–341
    [Google Scholar]
  28. Stein R. W., Corrigan M., Yaciuk P., Whelan J., Moran E. 1990; Analysis of ElA-mediated growth regulation functions: binding of the 300-kilodalton cellular product correlates with E1A enhancer repression function and DNA synthesis-inducing activity. Journal of Virology 64:4421–4427
    [Google Scholar]
  29. van Dam H., Offringa R., Smits A. M. A., Bos J. L., Jones N. C., van der Eb A. J. 1989; The repression of the growth factor-inducible genes JE, c-myc and stromelysin by adenovirus E1A is mediated by conserved region 1. Oncogene 4:1207–1212
    [Google Scholar]
  30. Vousden K. H., Jat P. S. 1989; Functional similarity between HPV16 E7, SV40 large T and adenovirus El A proteins. Oncogene 4:153–158
    [Google Scholar]
  31. Watanabe S., Kanda T., Sato H., Furano A., Yoshiike K. 1990; Mutational analysis of human papillomavirus type 16 E7 functions. Journal of Virology 64:207–214
    [Google Scholar]
  32. Whyte P., Buchkovich K. H., Horowitz J. M., Friend S. H., Raybuck M., Weinberg R. A., Harlow E. 1988; Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. Nature, London 334:124–129
    [Google Scholar]
  33. Whyte P., Williamson N. M., Harlow E. 1989; Cellular targets for transformation by the adenovirus El A proteins. Cell 56:67–75
    [Google Scholar]
  34. Yaciuk P., Moran E. 1991; Analysis with specific polyclonal antiserum indicates that the E1A-associated 300-kDa product is a stable nuclear phosphoprotein that undergoes cell cycle phase-specific modification. Molecular and Cellular Biology 11:5389–5397
    [Google Scholar]
  35. Yaciuk P., Corrigan Carter M., Pipas J. M., Moran E. 1991; Simian virus 40 large-T antigen expresses a biological activity complementary to the p300-associated transforming function of the adenovirus E1A gene products. Molecular and Cellular Biology 11:2116–2124
    [Google Scholar]
  36. Yee S., Branton P. 1985; Detection of cellular proteins associated with human adenovirus type 5 early region 1A polypeptides. Virology 147:142–153
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-8-2135
Loading
/content/journal/jgv/10.1099/0022-1317-73-8-2135
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error