1887

Abstract

We have made transgenic tobacco plants (, cv. Xanthi nc) expressing the movement protein (P3, 300 amino acids) of alfalfa mosaic virus (AlMV) and two N-terminally deleted proteins lacking respectively 12 and 77 amino acids of the P3 sequence (P3Δ[1–12] and P3Δ[1-77]). The same proteins were expressed in recombinant yeast. By subcellular fractionation, the full-length P3 protein expressed by transgenic plants was found to be associated with cell walls as well as with cytoplasmic particulate material, as was the wild type movement protein expressed by AlMV-infected tobacco plants. P3Δ[1-12] behaved similarly but P3Δ[1-77] was found only in the cytoplasm. It thus appears that a polypeptide domain located between amino acids 13 and 77 of the P3 sequence is necessary for association of the protein with cell walls.

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1992-08-01
2022-08-11
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References

  1. Atabekov J. G., Taliansky M. E. 1990; Expression of a plant-virus coded transport function by different viral genomes. Advances in Virus Research 38:201–248
    [Google Scholar]
  2. Barker R. F., Jarvis N. P., Thompson D. V., Loesch-Fries L. S., Hall T. C. 1983; Complete nucleotide sequence of alfalfa mosaic virus RNA3. Nucleic Acids Research 11:2881–2891
    [Google Scholar]
  3. Berna A., Briand J.-P., Stussi-Garaud C., Godefroy-Colburn T. 1986; Kinetics of accumulation of the three non-structural proteins of alfalfa mosaic virus in tobacco plants. Journal of General Virology 67:1135–1147
    [Google Scholar]
  4. Berna A., Gafny R., Wolf S., Lucas W. J., Holt C. A., Beachy R. N. 1991; The TM V movement protein: role of the C-terminal 73 amino-acids in subcellular localization and function. Virology 182:682–689
    [Google Scholar]
  5. Chevallier M. R., Bloch J. C., Lacroute F. 1980; Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeast. Gene 11:11–19
    [Google Scholar]
  6. Chou P. Y., Fasman G. D. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology 47:45–148
    [Google Scholar]
  7. Deom C. M., Schubert K. R., Wolf S., Holt C. A., Lucas W. J., Beachy R. N. 1990; Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proceedings of the National Academy of Sciences, U.S.A. 87:3284–3288
    [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  9. Dore J. -M., Pinck L. 1988; Plasmid DNA containing a copy of RNA3 can substitute for RNA3 in alfalfa mosaic virus RNA inocula. Journal of General Virology 69:1331–1338
    [Google Scholar]
  10. Dore J. -M., Erny C., Pinck L. 1990; Biologically active transcripts of alfalfa mosaic virus RNA3. FEBS Letters 264:183–186
    [Google Scholar]
  11. Dore J. -M., van Dun C. M. P., Pinck L., Bol J. F. 1991; Alfalfa mosaic virus RNA3 mutants do not replicate in transgenic plants expressing RNA3-specific genes. Journal of General Virology 72:253–258
    [Google Scholar]
  12. Engelman D. M., Steitz T. A., Goldman A. 1986; Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annual Review of Biophysics and Biophysical Chemistry 15:321–353
    [Google Scholar]
  13. Godefroy-Colburn T., Gagey M.-J., Berna A., Stussi-Garaud C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
    [Google Scholar]
  14. Godefroy-Colburn T., Schoumacher F., Erny C., Berna A., Moser O., Gagey M. J., Stussi-Garaud C. 1990; The movement protein of some plant viruses. In Recognition and Response in Plant-Virus Interactions NATO ASI series, vol H41 pp 207–231 Edited by Fraser R. S. S. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  15. Godefroy-Colburn T., Erny C., Schoumacher F., Berna A., Gagey M. -J., Stussi-Garaud C. 1991; Cell-to-cell movement of plant viruses. In Plant Molecular Biology 2 NATO ASI series, vol A212 pp 35–48 Edited by Herrmann R. G., Larkins B. A. New York: Plenum Press;
    [Google Scholar]
  16. Goldbach R., Eggen R., de Jager C., van Kammen A., van Lent J., Rezelman G., Wellink J. 1990; Genomic organization, evolution and expression of plant viral RNA genomes. In Recognition and Response in Plant-Virus Interactions NATO ASI series, vol H41 pp 147–162 Edited by Fraser R. S. S. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  17. Harrison B. D., Barker H., Derrick P. M. 1990; Intercellular spread of potato leafroll luteovirus: effects of co-infection and plant resistance. In Recognition and Response in Plant-Virus Interactions, NATO ASI series, vol. H41 pp 405–414 Edited by Fraser R. S. S. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  18. Horsch R. B., Fry J. E., Hoffmann N. L., Eichholtz D., Rogers S. G., Fraley R. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
    [Google Scholar]
  19. Hull R. 1989; The movement of viruses in plants. Annual Review of Phytopathology 27:213–240
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Moser O., Gagey M. -J., Godefroy-Colburn T., Stussi-Garaud C., Ellwart-Tschurtz M., Nitschko H., Mundry K. W. 1988; The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. Journal of General Virology 69:1367–1373
    [Google Scholar]
  22. Pinck L., Hirth L. 1972; The replicative RNA and the viral RNA synthesis rate in tobacco infected with alfalfa mosaic virus. Virology 49:413–425
    [Google Scholar]
  23. Ravelonandro M., Pinck M., Pinck L. 1984; Complete nucleotide sequence of RNA3 from alfalfa mosaic virus, strain S. Biochimie 66:395–402
    [Google Scholar]
  24. Rogers S. G., Horsch R. B., Fraley R. T. 1988; Gene transfer in plants: production of transformed plants using Ti plasmid vectors. In Methods for Plant Molecular Biology pp 423–436 Edited by Weissbach A., Weissbach H. New York: Academic Press;
    [Google Scholar]
  25. Sanders P. R., Winter J. A., Barnason A. R., Rogers S. G., Fraley R. T. 1987; Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants. Nucleic Acids Research 167:653–656
    [Google Scholar]
  26. Schoumacher F., Erny C., Berna A., Godefroy-Colburn T., Stussi-Garaud C. 1992; Nucleic acid-binding properties of the alfalfa mosaic virus movement protein produced in yeast. Virology 188:896–899
    [Google Scholar]
  27. Stussi-Garaud C., Garaud J. -C., Berna A., Godefroy-Colburn T. 1987; In situ location of an alfalfa mosaic virus nonstructural protein in plant cell walls: correlation with virus transport. Journal of General Virology 68:1779–1784
    [Google Scholar]
  28. van der Kuyl A. C., Neeleman L., Bol J. F. 1991; Deletion analysis of cis- and trans-acting elements involved in replication of alfalfa mosaic virus RNA3 in vivo . Virology 183:731–738
    [Google Scholar]
  29. van Lent J., Wellink J., Goldbach R. 1990; Evidence for the involvement of the 58K and 48K proteins in the intercellular movement of cowpea mosaic virus. Journal of General Virology 71:219–223
    [Google Scholar]
  30. Vernet T., Dignard D., Thomas D. Y. 1987; A family of yeast expression vectors containing the phage f1 intergenic region. Gene 52:225–233
    [Google Scholar]
  31. Wolf S., Deom C. M., Beachy R. N., Lucas W. J. 1989; Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246:377–379
    [Google Scholar]
  32. Yaffe M. P., Schatz G. 1984; Two nuclear mutations that block mitochondrial protein import in yeast. Proceedings of the National Academy of Sciences, U.S.A 81:4819–4823
    [Google Scholar]
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