1887

Abstract

Human papillomavirus (HPV) type 16 transcription was analysed by hybridization using I-labelled subgenomic riboprobes, from 26 genital intraepithelial neoplastic (IN) lesions, in formalin-fixed biopsies from 18 different cases. Distinct transcription patterns separable by the presence or absence of late gene transcription were detected. In 12 lesions, late gene expression was absent; HPV transcripts corresponding to the E6 and E7 open reading frame (ORF) were detectable in all basal cells and were usually evenly distributed through all layers of the epithelium. Transcripts corresponding to the E1, E2 and E2/E4 ORFs were present in nine of 12 lesions and displayed a similar distribution. In 14 lesions late gene transcripts were present. E6 and E7 transcripts were detectable basally in all but one lesion. The levels of E7 but not E6 transcripts were markedly increased in the superficial cells of differentiating epithelia, with an identical distribution and at similar levels to those of the E2/E4 transcript. We propose that the most abundant transcript in genital IN lesions containing late gene expression is an E7/E1 ^ E2/E4 transcript corresponding to that reported in HPV-6/11 condylomata and which is derived from a similar promoter within the E7 ORF.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-8-2047
1992-08-01
2022-12-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/8/JV0730082047.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-8-2047&mimeType=html&fmt=ahah

References

  1. Baker C. C., Howley P. M. 1987; Differential promoter utilization by the bovine papillomavirus in transformed cells and productively infected wart tissues. EMBO Journal 6:1027–1035
    [Google Scholar]
  2. Banks L., Matlashewski G., Pim D., Churcher M., Roberts C., Crawford L. 1987; Expression of human papillomavirus type 6 and type 16 capsid proteins in bacteria and their antigenic characterization. Journal of General Virology 68:3081–3089
    [Google Scholar]
  3. Broker T. R., Chow L. T., Chin M. T., Dong G., Rhodes C. R., Wolinsky S. M., Whitbeck A., Stoler M. H. 1989; A molecular portrait of human papillomavirus carcinogenesis. Cancer Cells 7:197–208
    [Google Scholar]
  4. Broker T. R., Chin M. T., Dong G., Rhodes C. R., Whitbeck A., Dollard S. C., Murant T., Ho T. F., Chung C.-M., Stoler M. H., Chow L. T. 1990; Papillomavirus transcriptional regulation in human genital neoplasia. Proceedings of the American Association for Cancer Research 31:488–490
    [Google Scholar]
  5. Chow L. T., Hirochika H., Nasseri M., Stoler M. H., Wolinsky S. M., Chin M. T., Hirochika R., Arvan D. S., Broker T. R. 1987a; Human papillomavirus gene expression. Cancer Cells 5:55–72
    [Google Scholar]
  6. Chow L. T., Nasseri M., Wolinsky S. M., Broker T. R. 1987b; Human papillomavirus types 6 and 11 mRNAs from genital condylomata acuminata. Journal of Virology 61:2581–2588
    [Google Scholar]
  7. Chow L. T., Reilly S. S., Broker T. R., Taichman L. B. 1987c; Identification and mapping of human papillomavirus type 1 RNA transcripts recovered from plantar warts and infected epithelial cell cultures. Journal of Virology 61:1913–1918
    [Google Scholar]
  8. Crum C. P., Nuovo G., Friedman D., Silverstein S. J. 1988; Accumulation of RNA homologous to human papillomavirus type 16 open reading frames in genital precancers. Journal of Virology 62:84–90
    [Google Scholar]
  9. Crum C. P., Symbula M., Ward B. E. 1989; Topography of early HPV 16 transcription in high-grade genital precancers. American Journal of Pathology 134:1183–1188
    [Google Scholar]
  10. Crum C. P., Barber S., Symbula M., Snyder K., Saleh A. M., Roches J. K. 1990; Coexpression of the human papillomavirus type 16 E4 and L1 open reading frames in early cervical neoplasia. Virology 178:238–246
    [Google Scholar]
  11. Doorbar J., Campbell D., Grand R. J. A., Gallimore P. H. 1986; Identification of the human papilloma virus-1a E4 gene products. EMBO Journal 5:355–362
    [Google Scholar]
  12. Doorbar J., Parton A., Hartley K., Banks L., Crook T., Stanley M., Crawford L. 1990; Detection of novel splicing patterns in a HPV 16-containing keratinocyte cell line. Virology 178:254–262
    [Google Scholar]
  13. Dürst M., Bosch F. X., Glitz D., Schneider A., zur Hausen H. 1991; Inverse relationship between human papillomavirus (HPV) type 16 early gene expression and cell differentiation in nude mouse epithelial cysts and tumor induced by HPV-positive human cell lines. Journal of Virology 65:796–804
    [Google Scholar]
  14. Higgins G. D., Davy M., Roder D., Uzelin D. M., Phillips G. E., Burrell C. J. 1991a; Increased age and mortality associated with cervical carcinomas negative for human papillomavirus RNA. Lancet 338:910–913
    [Google Scholar]
  15. Higgins G. D., Uzelin D. M., Phillips G. E., Burrell C. J. 1991b; Presence and distribution of human papillomavirus sense and antisense RNA transcripts in genital cancers. Journal of General Virology 72:885–895
    [Google Scholar]
  16. Higgins G. D., Uzelin D. M., Phillips G. E., Pieterse A. S., Burrell C. J. 1991c; Differing characteristics of human papillomavirus RNA-positive and RNA-negative anal carcinomas. Cancer 68:561–567
    [Google Scholar]
  17. Higgins G. D., Uzelin D. M., Phillips G. E., Villa L. L., Burrell C. J. 1992; Differing prevalences of human papillomavirus RNA in penile dysplasias and carcinomas may reflect differing aetiologies. American Journal of Clinical Pathology 97:272–277
    [Google Scholar]
  18. Kurman R. J., Schiffman M. H., Lancaster W. D., Reid R., Jenson A. B., Temple G. F., Lorincz A. T. 1988; Analysis of individual human papillomavirus types in cervical neoplasia: a possible role for type 18 in rapid progression. American Journal of Obstetrics and Gynecology 159:293–296
    [Google Scholar]
  19. Lorincz A. T., Temple G. F., Kurman R. J., Jenson A. B., Lancaster W. D. 1987; Oncogenic association of specific human papillomavirus types with cervical neoplasia. Journal of the National Cancer Institute 79:671–677
    [Google Scholar]
  20. McCancb D. J., Kopan R., Fuchs E., Laimins L. A. 1988; Human papillomavirus type 16 alters human epithelial cell differentiation in vitro . Proceedings of the National Academy of Sciences, U.S.A. 85:7169–7173
    [Google Scholar]
  21. McLean C. S., Churcher M. I., Meinke J., Smith G. L., Higgins G., Stanley M., Minson A. C. 1990; Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. Journal of Clinical Pathology 43:488–492
    [Google Scholar]
  22. Nasseri M., Hirochika R., Broker T. R., Chow L. T. 1987; A human papilloma virus type 11 transcript encoding an E1^E4 protein. Virology 159:433–439
    [Google Scholar]
  23. Pecoraro G., Morgan D., Defendi V. 1989; Differential effects of human papillomavirus type 6, 16, and 18 DNAs on immortalization and transformation of human cervical epithelial cells. Proceedings of the National Academy of Sciences, U.S.A. 86:563–567
    [Google Scholar]
  24. Reid R., Greenberg M., Jenson A. B., Husain M., Willett J., Daoud Y., Temple G., Stanhope C. R., Sherman A. I., Phibbs G. D., Lorincz A. T. 1987; Sexually transmitted papillomavirus infections. I. The anatomic distribution and pathologic grade of neoplastic lesions associated with different viral types. American Journal of Obstetrics and Gynecology 156:212–222
    [Google Scholar]
  25. Riou G., Favre M., Jeannel D., Bourhis J., le Doussal V., Orth G. 1990; Association between poor prognosis in early-stage invasive cervical carcinomas and non detection of HPV DNA. Lancet 335:1171–1174
    [Google Scholar]
  26. Rotenberg M. O., Chow L. T., Broker T. R. 1989; Characterization of rare human papillomavirus type 11 mRNAs coding for regulatory and structural proteins, using the polymerase chain reaction. Virology 172:489–497
    [Google Scholar]
  27. Seedorf K., Krammer G., Dürst M., Suhai S., Röwenkamp W. G. 1985; Human papillomavirus type 16 sequence. Virology 145:181–185
    [Google Scholar]
  28. Smotkin D., Prokoph H., Wettstein F. O. 1989; Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. Journal of Virology 63:1441–1447
    [Google Scholar]
  29. Stanley M. A., Browne H. M., Appelby M., Minson A. C. 1989; Properties of a non tumorigenic human cervical keratinocyte cell line. International Journal of Cancer 43:672–676
    [Google Scholar]
  30. Sterling J. C., Stanley M. A., Gatward G., Minson A. C. 1990; Production of HPV 16 virions by a human keratinocyte cell line. Journal of Virology 64:6305–6307
    [Google Scholar]
  31. Stoler M. H., Broker T. R. 1986; In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma. Human Pathology 17:1250–1258
    [Google Scholar]
  32. Stoler M. H., Wolinsky S. M., Whitbeck A., Broker T. R., Chow L. T. 1989; Differentiation-linked human papillomavirus types 6 and 11 transcription in genital condylomata revealed by in situ hybridization with message-specific RNA probes. Virology 172:331–340
    [Google Scholar]
  33. Stoler M. H., Rhodes C. R., Whitbeck A., Chow L. T., Broker T. R. 1990a; Gene expression of HPV types 16 and 18 in cervical neoplasia. In Papillomaviruses pp 1–11 Edited by Howley P. M. New York: Wiley-Liss;
    [Google Scholar]
  34. Stoler M. H., Whitbeck A., Wolinsky S. M., Broker T. R., Chow L. T., Howett M. K., Kreider J. W. 1990b; Infectious cycle of human papillomavirus type 11 in human foreskin xenografts in nude mice. Journal of Virology 64:3310–3318
    [Google Scholar]
  35. Vormwald-Dogan V., Fischer B., Bludau H., Freese U. K., Gissmann L., Glitz D., Schwarz E., Durst M. 1992; Sense and antisense transcripts of human papillomavirus type 16 in cervical cancers. Journal of General Virology 73:1833–1838
    [Google Scholar]
  36. Wettstein F. O., Barbosa M. S., Nasseri M. 1987; Identification of the major cottontail rabbit papillomavirus late RNA cap site and mapping and quantitation of an E2 and minor E6 coding mRNA in papillomas and carcinomas. Virology 159:321–328
    [Google Scholar]
  37. Woodworth C. D., Waggoner S., Barnes W., Stoler M. H., DiPaolo J. A. 1990; Human cervical and foreskin epithelial cells immortalized by human papillomavirus DNAs exhibit dysplastic differentiation in vivo . Cancer Research 50:3709–3715
    [Google Scholar]
  38. zur Hausen H. 1989; Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers. Cancer Research 49:4677–4681
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-8-2047
Loading
/content/journal/jgv/10.1099/0022-1317-73-8-2047
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error