1887

Abstract

A 6.4 kb region from the multicapsid nuclear polyhedrosis virus (LdMNPV) genome was sequenced and found to contain open reading frames (ORFs) homologous to the polyhedron envelope (PE) protein coding sequence, and the C-terminal half of ORF 1, which is a gene located upstream of the PE protein gene in other baculoviruses. The proteins predicted from the LdMNPV genes encoding the PE protein, and ORF 1 demonstrated 27 and 34% amino acid sequence identity, respectively, with the corresponding genes in the multicapsid nuclear polyhedrosis virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-6-1499
1992-06-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/6/JV0730061499.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-6-1499&mimeType=html&fmt=ahah

References

  1. Bjornson R. M., Rohrmann G. F. 1992; Nucleotide sequence of the p39-capsid gene region of the Lymantria dispar nuclear polyhedrosis virus. Journal of General Virology 72:1505–1508
    [Google Scholar]
  2. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555
    [Google Scholar]
  3. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 35:127–155
    [Google Scholar]
  4. Brutlag D. L., Dautricourt J.-P., Maulik S., Relph J. 1990; Sensitive similarity searches of biological sequence databases. Computer Applications in the Biosciences 6:237–245
    [Google Scholar]
  5. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabeling DNA restriction fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  6. Friesen P. D., Miller L. K. 1987; Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the Hin dIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 61:2264–2272
    [Google Scholar]
  7. Gombart A. F., Pearson M. N., Rohrmann G. F., Beaudreau G. S. 1989a; A baculovirus polyhedral envelope-associated protein: genetic location, nucleotide sequence and immunocytochemical characterization. Virology 169:182–193
    [Google Scholar]
  8. Gombart A. F., Blissard G. W., Rohrmann G. F. 1989b; Characterization of the genetic organization of the Hin dIII M region of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata reveals major differences among baculoviruses. Journal of General Virology 70:1815–1828
    [Google Scholar]
  9. Harrap K. A., Payne C. C., Robertson J. S. 1977; The properties of three baculoviruses from closely related hosts. Virology 79:14–31
    [Google Scholar]
  10. Henikoff S. 1987; Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods in Enzymology 155:156–165
    [Google Scholar]
  11. Hohn B., Collins J. 1980; A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
    [Google Scholar]
  12. Hoopes R. R. Jr, Rohrmann G. F. 1991; In vitro transcription of baculovirus immediate early genes: accurate mRNA initiation by nuclear extracts from both insect and human cells. Proceedings of the National Academy of Sciences, U.S.A. 88:4513–4517
    [Google Scholar]
  13. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  14. McCarthy W. J., Murphy T. F., Langridge W. 1979; Characteristics of the DNA from Lymantria dispar nuclear polyhedrosis virus. Virology 95:593–597
    [Google Scholar]
  15. Oellig C., Happ B., Muller T., Doerfler W. 1987; Overlapping sets of viral RNAs reflect the array of polypeptides in the Eco RI J and N fragments (map positions 81.2 to 85.0) of the Autographa californica nuclear polyhedrosis virus. Journal of Virology 61:3048–3057 [Authors’ correction 63, 1494 (1989).]
    [Google Scholar]
  16. Rohrmann G. F., Carnegie J. W., Martignoni M. E., Beaudreau G. S. 1977; Characterization of the genome of the nucleopolyhedrosis bundle virus pathogenic for Orgyia pseudotsugata. Virology 80:421–425
    [Google Scholar]
  17. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  18. Russell R. L. Q., Rohrmann G. F. 1990; A baculovirus polyhedron envelope protein: immunogold localization in infected cells and mature polyhedra. Virology 174:177–184
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Slavicek J. M. 1991; Temporal analysis and spatial mapping of Lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation products. Virus Research 20:223–236
    [Google Scholar]
  21. Smith I. R. L., Van Beek N. A. M., Podgwaite J. D., Wood H. A. 1988; Physical map and polyhedrin gene sequence of Lymantria dispar nuclear polyhedrosis virus. Gene 71:97–105
    [Google Scholar]
  22. Thiem S. M., Miller L. K. 1989b; A baculovirus gene with a novel transcription pattern encodes a polypeptide with a zinc finger and a leucine zipper. Journal of Virology 63:4489–4497
    [Google Scholar]
  23. Tjia S., Carstens E. B., Doerfler W. 1979; Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus. II. The viral DNA and kinetics of its replication. Virology 99:399–409
    [Google Scholar]
  24. Whitford M., Stewart S., Kuzio J., Faulkner P. 1989; Identification and sequence analysis of a gene encoding gp67 an abundant envelope glycoprotein of the baculovirus, Autographa californica nuclear polyhedrosis virus. Journal of Virology 63:1393–1399
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-6-1499
Loading
/content/journal/jgv/10.1099/0022-1317-73-6-1499
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error