1887

Abstract

This paper describes isolation and identification of a virus (termed strain 87A) which has the cytopathic effect and haemagglutination properties of hepatitis E virus (HEV). This virus was isolated by tissue culture from the faeces of a patient with acute non-A, non-B enteric hepatitis in Xinjiang, China. The isolated virus was neutralized by acute phase sera obtained from other patients with acute non-A, non-B enteric hepatitis. The virus particles also could be specifically aggregated with acute phase sera from patients with known HEV hepatitis in China, Burma, India and the U.S.S.R., and with acute and convalescent sera from an HEV-infected chimpanzee. Crystalline arrangements of virus particles in the cytoplasm were observed by electron microscopy in ultrathin sections of infected cells. The sedimentation coefficient of the strain 87A virus particles in sucrose gradients was 176S. Purified virus particles revealed a protein band of about 76K on SDS-PAGE and Western blotting. The evidence indicates that the strain 87A virus is an HEV. Our ability to propagate HEV in cell culture should facilitate research on this hepatotropic virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-5-1143
1992-05-01
2022-10-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/5/JV0730051143.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-5-1143&mimeType=html&fmt=ahah

References

  1. Andjaparidze A. G., Balayan M. S., Savinov A. P., Braginsky D.M., Poleschuk V. F., Zamyatina N. A. 1986; Fecal-orally transmitted non-A, non-B hepatitis induced in monkeys. Voprosy Virusologii 1:73–80
    [Google Scholar]
  2. Arankalle V. A., Ticehurst J., Sreenivasan M. A., Kapikian A. Z., Popper H., Pavri K. M., Purcell R. H. 1988; Aetiological association of a virus-like particle with enterically transmitted non-A, non-B hepatitis. Lancet i:550–554
    [Google Scholar]
  3. Balayan M. S., Andjaparidze A. G., Savinskaya S. S., Ketiladze E.S., Braginsky D. M., Savinovl A. P., Poleschuk V. F. 1983; Evidence for a virus in non-A, non-B hepsatitis transmitted via the fecal-oral route. Intervirology 20:23–31
    [Google Scholar]
  4. Bradley D. W. 1990; Enterically-transmitted non-A, non-B hepatitis. British Medical Bulletin 46:442–461
    [Google Scholar]
  5. Bradley D. W., Krawczynski K., Cook E. H., McCaustland K. A., Humphrey C. D., Spelbring J. E., Myint H., Maynard J. E. 1987; Enterically transmitted non-A, non-B hepatitis: serial passage of disease in cynomolgus macaques and tamarins and recovery of disease-associated 27 to 34 nm virus-like particles. Proceedings of the National Academy of Sciences, U.S.A. 84:6277–6281
    [Google Scholar]
  6. Bradley D. W., Andjaparidze A. G., Cook E. H. Jr, McCaustland K. A., Balayan M. S., Stetler H., Velazquez O., Robertson B., Humphrey C., Kane M., Weisfuse I. 1988; Aetiological agent of enterically transmitted non-A, non-B hepatitis. Journal of General Virology 69:731–738
    [Google Scholar]
  7. Cao X. Y., Ma X. Z., Liu Y. Z., Liu Z. E., Jin X. M., Gao Q., Dong H. J., Zhuang H., Liu C. B., Wang G. M. 1989; Studies of the epidemiology and aetiological agent of enterically transmitted non-A, non-B hepatitis in the south part of Xinjiang. Chinese Journal of Experimental and Clinical Virology 3:1–10
    [Google Scholar]
  8. Gust I. D., Purcell R. H. 1987; Report of a workshop: waterborne non-A, non-B hepatitis. Journal of Infectious Diseases 156:630–635
    [Google Scholar]
  9. Han Y. C., Huang R. T., Huang X. R., Wei J., Chen J. L., Huang Z. S. 1989; The buoyant density and structural proteins of human enteric coronavirus (HECV 86-2). Chinese Journal of Microbiology and Immunology 9:212–214
    [Google Scholar]
  10. Huang R. T., Li D. R., Huang Y. N. 1990a; Aetiological study of an outbreak of epidemic diarrhea caused by coronavirus. Chinese Journal of Epidemiology (supplement 8) 11:115–118
    [Google Scholar]
  11. Huang R. T., Li D. R., Yin S. R., Wei J., Tian X. 1990b; Preliminary report on a small RNA virus isolation from feces of a patient with enterically transmitted non-A, non-B hepatitis in China. Chinese Journal of Microbiology and Immunology 10:247
    [Google Scholar]
  12. Kane M. A., Bradley D. W., Shretha S. M., Maynard J. E., Cook E. H., Mishra P. P., Joshi D. D. 1984; Epidemic non-A, non-B hepatitis in Nepal: recovery of a possible etiologic agent and transmission studies in marmosets. Journal of the American Medical Association 252:3140–3145
    [Google Scholar]
  13. Khuroo M.S. 1980; Study of an epidemic of non-A, non-B hepatitis: possibility of another human hepatitis virus distinct from posttransfusion non-A, non-B type. American Journal of Medicine 68:818–824
    [Google Scholar]
  14. Panda S. K., Datta R., Kaur J., Zuckerman A. J., Nayak N. C. 1989; Enterically transmitted non-A, non-B hepatitis: recovery of virus-like particles from an epidemic in South Delhi and transmission studies in rhesus monkeys. Hepatology 10:466–472
    [Google Scholar]
  15. Reyes G. R., Purdy M., Kim J. S., Luk K. C., Young L. M., Fry K. E., Bradley D. W. 1990; Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science 247:1335–1339
    [Google Scholar]
  16. Tam A. W., Smith M. M., Guerra M. E., Huang C. C., Bradley D. W., Fry K. E., Reyes G. R. 1991; Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 185:120–131
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-5-1143
Loading
/content/journal/jgv/10.1099/0022-1317-73-5-1143
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error