The infectious genome of a Kenyan isolate of streak virus (PSV) has been cloned and sequenced. Infection of host plants was done using an binary vector containing a partial repeat of the genome. Progeny virus from resultant infections proved to be transmissible by the leafhopper (Naude). Comparisons of the amino acid sequences of PSV DNA-encoded proteins with those of previously characterized geminiviruses infecting monocotyledonous plants, including maize streak virus, revealed high levels of identity. The evolutionary relationship between PSV and other geminiviruses infecting monocotyledons is discussed.


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