1887

Abstract

Viral haemorrhagic septicaemia is the most important viral disease of trout in Europe. The causative agent, viral haemorrhagic septicaemia virus (VHSV), a member of the lyssavirus genus of the rhabdoviridae family, was formerly believed to be confined to portions of the European continent; however in 1988, VHSV was isolated from adult chinook () and coho () salmon returning to two hatcheries in the northwestern part of the State of Washington, U.S.A. Initial fears were that the virus had been imported into North America, perhaps by aquaculture activities. The nucleotide and deduced amino acid sequences of the nucleocapsid (N) gene of one of the North American (Makah) isolates of VHSV were determined and compared with published sequences of a European reference strain of VHSV (07-71) and the Round Butte strain of infectious haematopoietic necrosis virus (IHNV), another salmonid fish rhabdovirus that is enzootic in western North America. The N gene of the Makah isolate of VHSV shared a similarity of 88.433% at the nucleotide level and 94.802% at the amino acid level with the N gene of the European strain of VHSV, and 62.121% amino acid similarity with the N protein of IHNV. Like the European reference isolate, the North American isolate of VHSV showed three domains in the N protein, the central one being the most conserved and the likely site of interaction with genomic RNA. This was also the region of highest similarity with the amino acid sequence of IHNV. The sequence data suggested that the Makah and 07-71 isolates were of independent origin.

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1992-04-01
2022-01-18
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References

  1. Bernard J., Bearzotti-Le Berre M., de Kinkelin P. 1985; Viral haemorrhagic septicaemia in rainbow trout: attempt to relate interferon production, antibody synthesis and structure of the virus with the mechanism of virulence. Annates de I’Institut Pasteur/ Virologie 136E:13–26
    [Google Scholar]
  2. Bernard J., Lecocq-Xhonneux F., Rossius M., Thiry M. E., de Kinkelin P. 1990; Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus. Journal of General Virology 71:1669–1674
    [Google Scholar]
  3. Bilsel P. A., Rowe J. E., Fitch W. M., Nichol S. T. 1990; Phosphoprotein and nucleocapsid protein evolution of vesicular stomatitis virus New Jersey. Journal of Virology 64:2498–2504
    [Google Scholar]
  4. Brown C. M., Stockwell P. A., Trotman C. N., Tate W. P. 1990; Sequence analysis suggests that tetra-nucleotides signal the termination of protein synthesis in eukaryotes. Nucleic Acids Research 18:6339–6345
    [Google Scholar]
  5. Brunson R., True K., Yancey J. 1989; VHS virus isolated at Makah national fish hatchery. American Fisheries Society, Fish Health Section Newsletter 17:3–4
    [Google Scholar]
  6. Bullock W. O., Fernandez J. M., Short J. M. 1987; XLl-Blue, a high efficiency plasmid transforming rec A Escherichia coli strain with beta galactosidase selection. Focus 5376–378 Gaithersburg: Bethesda Research Laboratories;
    [Google Scholar]
  7. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  8. Crysler J. G., Lee P., Reinders M., Prevec L. 1990; The sequence of the nucleocapsid protein (N) gene of Piry virus: possible domains in the N protein of vesiculoviruses. Journal of General Virology 71:2191–2194
    [Google Scholar]
  9. de Kinkelin P., Chilmonczyk S., Dorson M., Le Berre M., Baudouy A. M. 1979; Some pathogenic facets of rhabdoviral infection of salmonid fish. In Munich Symposia on Microbiology: Mechanisms of Viral Pathogenesis and Vriulence p 357 Edited by Bachmann P. A.
    [Google Scholar]
  10. Fijan N., Sulmanovic M., Bearzotti M., Muzinic D., Zwillin-berg L. O., Chilmonczyk S., Vautherot J. F., de Kinkelin P. 1983; Some properties of the epithelioma papulosum cyprini (EPC) cell line from carp Cyprinus carpio . Annates de Virologie (Institut Pasteur) 134:207–220
    [Google Scholar]
  11. Gilmore R. D., Leong J. C. 1988; The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Virology 167:644–648
    [Google Scholar]
  12. Hattenberger-Baudouy A. M., Danton M., Merle G. 1989; Serological evidence of infectious hematopoietic necrosis in rainbow trout from a French outbreak of disease. Journal of Aquatic Animal Health 1:126–134
    [Google Scholar]
  13. Hopper K. 1989; The isolation of VHSV from chinook salmon at Glenwood Springs, Orcas Island, Washington. American Fisheries Society, Fish Health Section Newsletter 17:1–2
    [Google Scholar]
  14. Koener J. F., Passavant C. W., Kurath G., Leong J. 1987; Nucleotide sequence of a cDNA clone carrying the glycoprotein gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Journal of Virology 61:1342–1349
    [Google Scholar]
  15. Kozak M. 1987a; At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. Journal of Molecular Biology 196:947–950
    [Google Scholar]
  16. Kozak M. 1987b; An analysis of the 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8148
    [Google Scholar]
  17. Lazinski D., Grzadeielska E., Das A. 1989; Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators require a conserved arginine-rich motif. Cell 59:207–218
    [Google Scholar]
  18. McAllister P. E., Wagner R. R. 1975; Structural proteins of two salmonid rhabdoviruses. Journal of Virology 15:733–738
    [Google Scholar]
  19. Nichol S. T., Rowe J. E., Fitch W. M. 1989; Glycoprotein evolution of vesicular stomatitis virus New Jersey. Virology 168:281–291
    [Google Scholar]
  20. Short J. M., Fernandez J. M., Sorge J. A., Huse W. D. 1988; Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Research 16:7583–7600
    [Google Scholar]
  21. Thomas D., Newcomb W. W., Brown J. C., Wall J. S., Hainfeld J. F., Trus B. S., Steven A. C. 1985; Mass and molecular composition of vesicular stomatitis virus: a scanning transmission electron microscopy analysis. Journal of Virology 54:598–607
    [Google Scholar]
  22. Tordo N., Poch O., Ermine A., Keith G. 1986a; Primary structure of leader RNA and nucleoprotein genes of the rabies genome: segmented homology with VSV. Nucleic Acids Research 14:2671–2683
    [Google Scholar]
  23. Tordo N., Poch O., Ermine A., Keith G., Rougeon F. 1986b; Walking along the rabies genome: is the large G-L intergenic region a remnant gene?. Proceedings of the National Academy of Sciences, U.S.A 83:3914–3918
    [Google Scholar]
  24. Winton J. R., Batts W. N., Nishizawa T., Stehr C. 1989; Characterization of the first North American isolates of viral hemorrhagic septicemia virus. American Fisheries Society, Fish Health Section Newsletter 17:2–3
    [Google Scholar]
  25. Wolf K. 1988 Fish Viruses and Fish Viral Diseases pp 217–249 New York: Cornell University Press;
    [Google Scholar]
  26. Zamore P. D., Zapp M. L., Green M. R. 1990; Gene expression. RNA binding Bs and basics. Nature, London 348:485–486
    [Google Scholar]
  27. Zopf D., Bernstein H. D., Johnson A. E., Walter P. 1990; The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence. EMBO Journal 9:4511–4517
    [Google Scholar]
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