1887

Abstract

A full-length cDNA clone of an aphid non-transmissible isolate of plum pox potyvirus (PPV) was rendered biologically active when placed under the control of the cauliflower mosaic virus 35S RNA promoter and the nopaline synthase polyadenylation signal. The cDNA was constructed so that the exact 5′ end of the PPV RNA was present at the transcription initiation site. Inoculation of plasmid DNA onto led to systemic infection, whereas local lesions were produced in and , typical of an infection with PPV. Examination of infected plants revealed PPV-specific virus particles as well as viral RNA, the coat protein and the non-structural large nuclear inclusion protein (NIb).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-3-709
1992-03-01
2022-08-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/3/JV0730030709.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-3-709&mimeType=html&fmt=ahah

References

  1. Adam G., Chagas C. M., Lesemann D.-E. 1987; Comparison of three plant rhabdovirus isolates by two different serological techniques. Journal of Phytopathology 120:31–43
    [Google Scholar]
  2. Allison R., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  3. Balint R., Plooy I., Steele C. 1990; The nucleotide sequence of zucchini yellow mosaic virus. Abstracts of the VIIIth International Congress of Virology p 472
    [Google Scholar]
  4. Casper R., Meyer S. 1981; Die Anwendung des ELISA-Verfahrens zum Nachweis pflanzenpathogener Viren. Mitteilungen des Deutschen Pflanzenschutzdienstes 33:49–54
    [Google Scholar]
  5. Commandeur U., Jarausch W., Li Y., Koenig R., Burgermeister W. 1991; cDNAs of beet necrotic yellow vein virus RNAs 3 and 4 are rendered biologically active in a plasmid containing the cauliflower mosaic virus 35S promoter. Virology 185:493–495
    [Google Scholar]
  6. Crees D. E. M., Kiefer M. C., Owens R. A. 1983; Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Research 11:6821–6825
    [Google Scholar]
  7. de Zoeten G. A. 1991; Risk assessment: do we let history repeat itself?. Phytopathology 81:585–586
    [Google Scholar]
  8. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmeyer J. H., Hiremath S. T., Siaw M. F. E., Lomonosoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5429
    [Google Scholar]
  9. Dore J.-M., Pinck L. 1988; Plasmid DNA containing a copy of RNA3 can substitute for RNA3 in alfalfa mosaic virus RNA inocula. Journal of General Virology 69:1331–1338
    [Google Scholar]
  10. Hollings M., Brunt A. A. 1981; Potyviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 731–807 Edited by Kurstak E. New York: Elsevier/North-Holland;
    [Google Scholar]
  11. Janda M., French R., Ahlquist P. 1987; High efficiency T7 polymerase synthesis of infectious RNA from cloned brome mosaic virus cDNA and effects of 5′ extensions on transcript infectivity. Virology 158:259–262
    [Google Scholar]
  12. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods in Enzymology 154:367–382
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Laín S., Riechmann J. L., García J. A. 1989; The complete nucleotide sequence of plum pox potyvirus RNA. Virus Research 13:157–172
    [Google Scholar]
  15. Maiss E., Breyel E., Brisske A., Casper R. 1988; Molecular cloning of DNA complementary to the RNA-genome of plum pox virus (PPV). Journal of Phytopathology 122:222–231
    [Google Scholar]
  16. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. W. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  17. Milne R. G., Lesemann D.E. 1984; Immunosorbent electron microscopy in plant virus studies. Methods in Virology 3:85–101
    [Google Scholar]
  18. Mori M., Mise K., Kobayashi K., Okuno T., Furusawa I. 1991; Infectivity of plasmids containing brome mosaic virus cDNA linked to the cauliflower mosaic virus 35S RNA promoter. Journal of General Virology 72:243–246
    [Google Scholar]
  19. Riechmann J. L., Laín S., García J. A. 1990; Infectious in vitro transcripts from a plum pox potyvirus cDNA clone. Virology 177:710–716
    [Google Scholar]
  20. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Van Emmelo J., Ameloot P., Fiers W. 1987; Expression in plants of the cloned satellite tobacco necrosis virus genome and of derived insertion mutants. Virology 157:480–487
    [Google Scholar]
  23. Van Etten J. L., Freer S. N., McCune B. K. 1979; Presence of a major (storage?) protein in dormant spores of the fungus Botryodiplodia theobromae . Journal of Bacteriology 138:650–652
    [Google Scholar]
  24. Verwoerd T. C., Dekker B. M. M., Hockema A. 1989; A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17:2362
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-3-709
Loading
/content/journal/jgv/10.1099/0022-1317-73-3-709
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error