1887

Abstract

Based on variation in nucleotide sequence within restricted regions in the putative C (core) gene of hepatitis C virus (HCV), four groups of HCV have been postulated in a panel of 44 HCV isolates. They were provisionally designated types I, II, III and IV. A method for typing HCV was developed, depending on the amplification of a C gene sequence by polymerase chain reaction using a universal primer (sense) and a mixture of four type-specific primers (antisense). HCV types were determined by the size of the products specific to each of them. Type II was found in HCV samples from 131 (82%) of 159 blood donors, more often than in those from 48 (60%) of 80 patients with non-A, non-B (NANB) liver disease in Japan ( < 0.01). In 11 haemophiliacs who had received imported coagulation factor concentrates, type I was found in five, as against type II in four. Double infection with two different HCV types was found in two patients with chronic NANB liver disease (types I and II; II and III) and two haemophiliacs (types I and II; I and III). HCV types were identical in mother and baby in each of two examples of perinatal transmission, and were also identical in donor and recipient in a case of accidental needle exposure.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-3-673
1992-03-01
2022-08-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/3/JV0730030673.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-3-673&mimeType=html&fmt=ahah

References

  1. Alter H. J., Purcell R. H., Shih J. W., Melpolder J. C., Houghton M., Choo Q.-L., Kuo G. 1989a; Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. New England Journal of Medicine 321:1494–1500
    [Google Scholar]
  2. Alter M. J., Coleman P. J., Alexander W. J., Kramer E., Miller J. K., Mandel E., Hadler S. C., Margolis H. S. 1989b; Importance of heterosexual activity in the transmission of hepatitis B and non-A, non-B hepatitis. Journal of the American Medical Association 262:1201–205
    [Google Scholar]
  3. Alter M. J., Hadler S. C., Judson F. N., Mares A., Alexander W. J., Hu P. Y., Miller J. K., Moyer L. A., Fields H. A., Bradley D. W., Margolis H. S. 1990; Risk factors for acute non-A, non-B hepatitis in the United States and association with hepatitis C virus infection. Journal of the American Medical Association 264:2231–2235
    [Google Scholar]
  4. Bruix J., Barrera J. M., Calvet X., Ercilla G., Costa J., Sanchez-Tapias J. M., Ventura M., Vall M., Bruguera M., Bru C., Castillo R., Rodes J. 1989; Prevalence of antibodies to hepatitis C virus in Spanish patients with hepatocellular carcinoma and hepatic cirrhosis. Lancet ii:1004–1006
    [Google Scholar]
  5. Choo Q.-L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362
    [Google Scholar]
  6. Choo Q.-L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby A., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A 88:2451–2455
    [Google Scholar]
  7. Deubel V., Laille M., Hugnot J.-P., Chungue E., Guesdon J.-L., Drouet M. T., Bassot S., Chevrier D. 1990; Identification of dengue sequences by genomic amplification: rapid diagnosis of dengue virus serotypes in peripheral blood. Journal of Virological Methods 30:41–54
    [Google Scholar]
  8. Enomoto N., Takada A., Nakao T., Date T. 1990; There are two major types of hepatitis C virus in Japan. Biochemical and Biophysical Research Communications 170:1021–1025
    [Google Scholar]
  9. Esteban J. I., Esteban R., Viladomiu L., Lopez-Talavera J. C., Gonzalez A., Hernandez J. M., Roget M., Vargas V., Genesca J., Buti M., Guardia J., Houghton M., Choo Q. L., Kuo G. 1989; Hepatitis C virus antibodies among risk groups in Spain. Lancet ii:294–297
    [Google Scholar]
  10. Farci P., Alter H. J., Wong D., Miller R. H., Shih J. W., Jett B., Purcell R. H. 1991; A long-term study of hepatitis C virus replication in non-A, non-B hepatitis. New England Journal of Medicine 325:98–104
    [Google Scholar]
  11. Garson J. A., Preston F. E., Makris M., Tuke P., Ring C., Machin S. J., Tedder R. S. 1990; Detection by PCR of hepatitis C virus in factor VIII concentrates. Lancet 335:1473
    [Google Scholar]
  12. Hijikata M., Kato N., Mori S., Ootsuyama Y., Nakagawa M., Sugimura T., Ohkoshi S., Kojima H., Meguro T., Taki M., Takayama S., Yamada K., Shimotohno K. 1990; Frequent detection of hepatitis C virus US strain in Japanese hemophiliacs. Japanese Journal of Cancer Research 81:1195–1197
    [Google Scholar]
  13. Kaneko S., Unoura M., Kobayashi K., Kuno K., Murakami S., Hattori N. 1990; Detection of serum hepatitis C virus RNA. Lancet 335:976
    [Google Scholar]
  14. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. 1990; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proceedings of the National Academy of Sciences, U.S.A 87:9524–9528
    [Google Scholar]
  15. Kubo Y., Takeuchi K., Boonmar S., Katayama T., Choo Q.-L., Kuo G., Weiner A. J., Bradley D. W., Houghton M., Saito I., Miyamura T. 1989; A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan. Nucleic Acids Research 17:10367–10372
    [Google Scholar]
  16. Kuo G., Choo Q.-L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E., Tegtmeier G. E., Bonino F., Colombo M., Lee W.-S., Kuo C., Berger K., Shuster J. R., Overby L. R., Bradley D. W., Houghton M. 1989; An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362–364
    [Google Scholar]
  17. Miyamura T., Saito L., Katayama T., Kikuchi S., Tateda A., Houghton M., Choo Q. L., Kuo G. 1990; Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis. Proceedings of the National Academy of Sciences, U.S.A 87:983–987
    [Google Scholar]
  18. Norder H., Hammas B., Magnius L. O. 1990; Typing of hepatitis B virus genomes by a simplified polymerase chain reaction. Journal of Medical Virology 31:215–221
    [Google Scholar]
  19. Ogata N., Alter H. J., Miller R. H., Purcell R. H. 1991; Nucleotide sequence and mutation rate of the H strain of hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A 88:3392–3396
    [Google Scholar]
  20. Okamoto H., Okada S., Sugiyama Y., Tanaka T., Sugai Y., Akahane Y., Machida A., Mishiro S., Yoshizawa H., Miyakawa Y., Mayumi M. 1990a; Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5′-noncoding region. Japanese Journal of Experimental Medicine 60:215–222
    [Google Scholar]
  21. Okamoto H., Okada S., Sugiyama Y., Yotsumoto S., Tanaka T., Yoshizawa H., Tsuda F., Miyakawa Y., Mayumi M. 1990b; The 5′-terminal sequence of the hepatitis C virus genome. Japanese Journal of Experimental Medicine 60:167–177
    [Google Scholar]
  22. Okamoto H., Okada S., Sugiyama Y., Kurai K., Iizuka H., Machida A., Miyakawa Y., Mayumi M. 1991; Nucleotide sequence of the genomic RNA of hepatitis C virus isolated from a human carrier: comparison with reported isolates for conserved and divergent regions. Journal of General Virology 72:2697–2704
    [Google Scholar]
  23. Reesink H. W., Wong V. C. W., Ip H. M. H., van der Poel C. L., van Exel-Oehlers P. J., Lelie P. N. 1990; Mother-to-infant transmission and hepatitis C virus. Lancet 335:1216–1217
    [Google Scholar]
  24. Simmonds P., Zhang L. Q., Watson H. G., Rebus S., Ferguson E. D., Balfe P., Leadbetter G. H., Yap P. L., Peutherer J. F., Ludlam C. A. 1990; Hepatitis C quantification and sequencing in blood products, haemophiliacs, and drug users. Lancet 336:1469–1472
    [Google Scholar]
  25. Stevens C. E., Taylor P. E., Pindyck J., Choo Q.-L., Bradley D. W., Kuo G., Houghton M. 1990; Epidemiology of hepatitis C virus. A preliminary study in volunteer blood donors. Journal of the American Medical Association 263:49–53
    [Google Scholar]
  26. Tajima K., Shimotohno K., Oki S. 1991; Natural horizontal transmission of HCV in microepidemic town in Japan. Lancet 337:1410–1411
    [Google Scholar]
  27. Takamizawa A., Mori C., Fuke I., Manabe S., Murakami S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. 1991; Structure and organization of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65:1105–1113
    [Google Scholar]
  28. Takeuchi K., Kubo Y., Boonmar S., Watanabe Y., Katayama T., Choo Q. L., Kuo G., Houghton M., Saito I., Miyamura T. 1990; The putative nucleocapsid and envelope protein genes of hepatitis C virus determined by comparison of the nucleotide sequences of two isolates derived from an experimentally infected chimpanzee and healthy human carriers. Journal of General Virology 71:3027–3033
    [Google Scholar]
  29. Ulrich P. P., Romeo J. M., Lane P. K., Kelly L., Daniel L. J., Vyas G. N. 1990; Detection, semiquantitation, and genetic variation in hepatitis C virus sequences amplified from the plasma of blood donors with elevated alanine aminotransferase. Journal of Clinical Investigation 861609–1614
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-3-673
Loading
/content/journal/jgv/10.1099/0022-1317-73-3-673
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error