1887

Abstract

The effects of human immunodeficiency virus type 1 (HIV-1) and recombinant envelope glycoprotein gp120 on the growth of enriched human haematopoietic progenitors (CD34 cells) have been investigated. A 2 h exposure to HIV-1 resulted in a progressive and significant reduction of viable CD34 cell number in liquid cultures and of granulocyte-macrophage, erythroid and megakaryocytic progenitors in semisolid cultures. In virus-treated CD34 cells, no signs of active virus replication were observed and the possibility of latent infection was excluded by quantitative polymerase chain reaction. Recombinant HIV-1 envelope glycoprotein gp120 added to CD34 cell cultures displayed a dose-dependent inhibitory activity on CD34 cell viability. Neutralizing antibody against gp120 was able to block completely the inhibitory activity on CD34 cells of either HIV-1 or recombinant gp120. These results demonstrate that HIV-1 envelope glycoprotein gp120 has a direct cytotoxic effect on CD34 cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-2-417
1992-02-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/2/JV0730020417.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-2-417&mimeType=html&fmt=ahah

References

  1. Bagnara G. P., Zauli G., Giovannini M., Re M. C., Furlini G., La Placa M. 1990; Early loss of circulating hemopoietic progenitors in HIV-1 infected subjects. Experimental Hematology 18:426–430
    [Google Scholar]
  2. Carlo Stella C., Ganser A., Hoelzer D. 1987; Defective in vitro growth of the hemopoietic progenitor cells in the acquired immunodeficiency syndrome. Journal of Clinical Investigation 80:286–293
    [Google Scholar]
  3. Davis B. R., Marx J. C., Johnson C. E., Berry J. M., Lyding J., Zander A., Merigan T. C., Schwartz D. H. 1991; Absent or rare HIV infection of bone marrow stem/progenitor cells in vivo. Journal of Virology 65:1985–1990
    [Google Scholar]
  4. Dianzani F., Antonelli G., Capobianchi M. R., Demarco F. 1988; Replication of human immunodeficiency virus: yield of infectious virus under single growth cycle conditions. Archives of Virology 103:127–131
    [Google Scholar]
  5. Folks T. M. 1986; Biological and biochemical characterization of a cloned Leu-3_ cell surviving infection with the acquired immune deficiency syndrome retrovirus. Journal of Experimental Medicine 164:280–290
    [Google Scholar]
  6. Folks T. M. 1991; Human immunodeficiency virus in bone marrow: still more questions than answers. Blood 77:1625–1626
    [Google Scholar]
  7. Folks T. M., Kessler S. W., Orenstein J. M., Justment J. S., Laffe E., Fauci A. S. 1988; Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow. Science 242:919–922
    [Google Scholar]
  8. Ganser A. 1988; Abnormalities of hematopoiesis in the acquired immunodeficiency syndrome. Blut 56:49–55
    [Google Scholar]
  9. Kaplan D. R., Henkel T. J., Braciale V., Braciale J. T. 1984; Mycoplasma infection of cell cultures: thymidine incorporation of culture supernatants as a screening test. Journal of Immunology 132:9–11
    [Google Scholar]
  10. Kitano K., Abboud C. N., Ryan D. H., Quan S. C., Baldwin G. C., Golde D. W. 1991; Macrophage-active colony-stimulating factors enhance human immunodeficiency virus type 1 infection in bone marrow stem cells. Blood 77:1699–1706
    [Google Scholar]
  11. Lake J. P., Lee S., Spira S. 1984; Bone marrow findings in AIDS patients. American Journal of Clinical Pathology 81:799–800
    [Google Scholar]
  12. Leiderman I. Z., Greenberg M. L., Adelsberg B. R., Siegal F. P. 1987; A glycoprotein inhibitor of in vitro granulopoiesis associated with AIDS. Blood 70:1267–1272
    [Google Scholar]
  13. Mann D. L., O’Brien S. J., Gilbert D. A., Reid Y., Popovic M., Read-Connole E., Gallo R. C., Gazdar A. F. 1989; Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Research and Human Retroviruses 5:253–255
    [Google Scholar]
  14. Molina J. M., Scadden D. T., Sakaguchi M., Fuller B., Woon A., Groopman J. E. 1990; Lack of evidence for infection of or effect on growth of hematopoietic progenitor cells after in vivo or in vitro exposure to human immunodeficiency virus. Blood 76:2476–2482
    [Google Scholar]
  15. Ou C. Y., Kwok S., Mitchell S. W., Mack D. H., Sninsky J. J., Krebs J. W., Feorino P., Warfield D., Schochetman G. 1988; DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science 239:295–297
    [Google Scholar]
  16. Re M. C., Furlini G., La Placa M. 1989; Rapid detection of HIV-1 in clinical samples by co-culture with heat-shocked cells. Journal of Virological Methods 26:313–318
    [Google Scholar]
  17. Scadden D. T., Zon L. I., Groopman J. E. 1989; Pathophysiology and management of HIV-associated hematologic disorders. Blood 4:1455–1463
    [Google Scholar]
  18. Scharf S. J., Horn G. T., Erlich H. A. 1986; Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 133:1076–1078
    [Google Scholar]
  19. Schneider D. R., Picker L. J. 1985; Myelodysplasia in the acquired immunodeficiency syndrome. American Journal of Clinical Pathology 84:144–152
    [Google Scholar]
  20. Spivak J. L., Selonik S. E., Quinn T. C. 1983; Acquired immunodeficiency syndrome and pancytopenia. Journal of the American Medical Association 250:3084–3087
    [Google Scholar]
  21. Spivak J. L., Bender B. S., Quinn T. C. 1984; Hematological abnormalities in AIDS. American Journal of Medicine 77:224–228
    [Google Scholar]
  22. Steinberg H. N., Crumpacker C. S., Chatis P. A. 1991; In vitro suppression of normal human bone marrow progenitor cells by human immunodeficiency virus. Journal of Virology 65:1765–1769
    [Google Scholar]
  23. Treacy M., Lai L., Costello C., Clark A. 1987; Peripheral blood and bone marrow abnormalities in patients with HIV-related diseases. British Journal of Haematology 65:289–294
    [Google Scholar]
  24. Von Laer D., Hufert F. T., Fenner T. E., Schwander S., Dietrich M., Schmitz H., Kern P. 1990; CD34+ hematopoietic progenitor cells are not a major reservoir of the human immunodeficiency virus. Blood 76:1281–1286
    [Google Scholar]
  25. Zon L. L., Arkin C., Groopman J. E. 1987; Hematologic manifestations of the human immunodeficiency virus (HIV). British Journal of Haematology 66:251–256
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-2-417
Loading
/content/journal/jgv/10.1099/0022-1317-73-2-417
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error