1887

Abstract

The non-structural protein NS1 of influenza A virus exhibits two modes of RNA-binding activity. One is sequence-specific binding to minus-sense virus RNA with either a 5′- or 3′-terminal common sequence as reported previously. The other was identified as binding to dsRNA and this activity did not show sequence specificity. The affinity of binding to dsRNA was much higher than that to ssRNA. A short miniature virion RNA forming a panhandle structure by pairing between the 5′- and 3′-terminal common sequences bound NS1 with higher affinity and stability than did a dsRNA of similar sequence and length.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3325
1992-12-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123325.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3325&mimeType=html&fmt=ahah

References

  1. Alonso-Caplen F. V., Nemeroff M. E., Qiu Y., Krug R. M. 1992; Nucleocytoplasmic transport: the influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA. Genes and Development 6:255–267
    [Google Scholar]
  2. Bartel D. P., Zapp M. L., Green M. R., Szostak J. W. 1991; HIV-1 Rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA. Cell 67:529–536
    [Google Scholar]
  3. Calnan B. J., Tidor B., Biancalana S., Hudson D., Frankel A. D. 1991; Arginine-mediated RNA recognition: the arginine fork. Science 252:1167–1171
    [Google Scholar]
  4. Enami M., Palese P. 1991; High-efficiency formation of influenza virus transfectants. Journal of Virology 65:2711–2713
    [Google Scholar]
  5. Hatada E., Hasegawa M., Shimizu K., Hatanaka M., Fukuda R. 1990; Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8. Journal of General Virology 71:1283–1292
    [Google Scholar]
  6. Hatada E., Takizawa T., Fukuda R. 1992; Specific binding of influenza A virus NS1 protein to the virus minus-sense RNA in vitro . Journal of General Virology 13:17–25
    [Google Scholar]
  7. Hsu M. T., Parvin J. D., Gupta S., Krystal M., Palese P. 1987; Genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle. Proceedings of the National Academy of Sciences, U.S.A. 84:8140–8144
    [Google Scholar]
  8. Inglis S. C., Gething M.-J., Brown C. M. 1980; Relationship between the messenger RNAs transcribed from two overlapping genes of influenza virus. Nucleic Acids Research 8:3575–3589
    [Google Scholar]
  9. Lamb R. A. 1989; Genes and proteins of the influenza viruses. In The Influenza Viruses pp. 1–87 Edited by Krug R. M. New York & London: Plenum Press;
    [Google Scholar]
  10. Lamb R. A., Lai C.-J. 1980; Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus. Cell 21475–485
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3325
Loading
/content/journal/jgv/10.1099/0022-1317-73-12-3325
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error