1887

Abstract

The DNA polymerase gene of the multinucleocapsid nuclear polyhedrosis virus (LdMNPV) was cloned and sequenced. The predicted DNA polymerase protein (1113 amino acids, 115.9K) was found to have an amino acid identity of 48% with the corresponding gene of the MNPV (AcMNPV). It contains five domains associated with substrate binding, primase interaction, and pyrophosphate hydrolysis and three domains associated with 3′»5′ exonuclease activity common to other DNA polymerases. A region with a conserved TATA promoter and a CAGT mRNA start site sequence motif was identified and shown to be transcribed by RNA polymerase II, indicating that the LdMNPV DNA polymerase gene is expressed as an early gene. An open reading frame possibly expressed as a late gene, oriented in the opposite direction and overlapping the N-terminal coding region of the DNA polymerase gene was found in the LdMNPV sequence and was shown to be conserved in the same position in AcMNPV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3177
1992-12-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123177.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3177&mimeType=html&fmt=ahah

References

  1. Bjornson R. M., Rohrmann G. F. 1992a; Nucleotide sequence of the p39-capsid gene region of the Lymantria dispar nuclear polyhedrosis virus. Journal of General Virology 73:1505–1508
    [Google Scholar]
  2. Bjornson R. M., Rohrmann G. F. 1992b; Nucleotide sequence of the polyhedron envelope gene region of the Lymantria dispar nuclear polyhedrosis virus. Journal of General Virology 73:1499–1504
    [Google Scholar]
  3. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555
    [Google Scholar]
  4. Brutlag D. L., Dautricourt J.-P., Maulik S., Relph J. 1990; Sensitive similarity searches of biological sequence databases. Computer Applications in the Biosciences 6:237–245
    [Google Scholar]
  5. Chang M. T., Lanner-Herrera C., Fixes M. 1989; Nucleotide sequence of Lymantria dispar nuclear polyhedrosis virus polyhedrin gene. Journal of Invertebrate Pathology 53:241–246
    [Google Scholar]
  6. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabeling DNA restriction fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  7. Glocker B., Hoopes R. R. Jr, Rohrmann G. F. 1992; In vitro transactivation of baculovirus early genes by nuclear extracts from Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells. Journal of Virology 66:3476–3484
    [Google Scholar]
  8. Gombart A. F., Blissard G. W., Rohrmann G. F. 1989; Characterization of the genetic organization of the Hind III M region of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata reveals major differences among baculoviruses. Journal of General Virology 70:1815–1828
    [Google Scholar]
  9. Henikoff S. 1987; Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods in Enzymology 155:156–165
    [Google Scholar]
  10. Hohn B., Collins J. 1980; A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
    [Google Scholar]
  11. Hoopes R. R. Jr, Rohrmann G. F. 1991; In vitro transcription of baculovirus immediate early genes: accurate mRNA initiation by nuclear extracts from both insect and human cells. Proceedings of the National Academy of Sciences, U.S.A. 88:4513–4517
    [Google Scholar]
  12. Kozak M. 1986; Point mutations define a sequence flanking the AUG initator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  13. McCarthy W. J., Murphy T. F., Langridge W. 1979; Characteristics of the DNA from Lymantria dispar nuclear polyhedrosis virus. Virology 95:593–597
    [Google Scholar]
  14. Mikhailov V. S., Marlyev K. A., Ataeva J. O., Kullyev P. K., Atrazhev A. M. 1986; Characterization of 3′ to 5′ exonuclease associated with DNA polymerase of silkworm nuclear polyhedrosis virus. Nucleic Acids Research 14:3841–3856
    [Google Scholar]
  15. Morrison A., Bell J. B., Kunkel T. A., Sugino A. 1991; Eukaryotic DNA polymerase amino acid sequence required for 3′ to 5′ exonuclease activity. Proceedings of the National Academy of Sciences, U.S.A. 88:9473–9477
    [Google Scholar]
  16. Ooi B. G., Miller L. K. 1990; Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream. Journal of Virology 64:3126–3129
    [Google Scholar]
  17. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  18. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Slavicek J. M. 1991; Temporal analysis and spatial mapping of Lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation products. Virus Research 20:223–236
    [Google Scholar]
  20. Smith I. R. L., Van Beek N. A. M., Podgwaite J. D., Wood H. A. 1988; Physical map and polyhedrin gene sequence of Lymantria dispar nuclear polyhedrosis virus. Gene 71:97–105
    [Google Scholar]
  21. Thiem S. M., Miller L. K. 1989; A baculovirus gene with a novel transcription pattern encodes a polypeptide with a zinc finger and a leucine zipper. Journal of Virology 63:4489–4497
    [Google Scholar]
  22. Tomalski M. D., Wu J., Miller L. K. 1988; The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene. Virology 167:591–600
    [Google Scholar]
  23. Vialard J. E., Yuen L., Richardson C. D. 1990; Identification and characterization of a baculovirus occlusion body glycoprotein which resembles spheroidin, an entomopoxvirus protein. Journal of Virology 64:5804–5811
    [Google Scholar]
  24. Wang T. S.-F., Wong S. W., Korn D. 1989; Human DNA polymerase α: predicted functional domains and relationships with viral DNA polymerases. FASEB Journal 3:14–21
    [Google Scholar]
  25. Wu J., Miller L. K. 1989; Sequence, transcription and translation of a late gene of the Autographa californica nuclear polyhedrosis virus encoding a 34.8K polypeptide. Journal of General Virology 70:2449–2459
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3177
Loading
/content/journal/jgv/10.1099/0022-1317-73-12-3177
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error