1887

Abstract

During an active infection with human cytomegalovirus (HCMV), viral antigen is consistently present in peripheral blood leukocytes. Two monoclonal antibodies (MAbs), CMV-C10 and CMV-C11, are commonly used in the HCMV antigenaemia assay to detect these cells in the peripheral blood of patients suspected of having an active HCMV infection. We demonstrate that the viral antigen detected by these MAbs is the viral structural protein pp65 and not an immediate early antigen as previously reported. Furthermore, significantly fewer leukocytes were found to be positive with MAbs specific for immediate early antigens.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-11-2923
1992-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/11/JV0730112923.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-11-2923&mimeType=html&fmt=ahah

References

  1. Adler S. P. 1983; Transfusion-associated cytomegalovirus infections. Reviews of Infectious Diseases 5:977–993
    [Google Scholar]
  2. Amadei C., Tardy-Panit M., Couillin P., Coulon M., Cabau N., Boue A., Michelson S. 1983; Kinetic study of the development and localization of human cytomegalovirus-induced antigens using monoclonal antibodies. Annates de Virologie 134E:165–180.
    [Google Scholar]
  3. Boland G. J., de Gast G. C., Hene R. J., Jambroes G., Doncker-Wolcke R., The T. H., Mudde G. C. 1990; Early detection of active cytomegalovirus (CMV) infection after heart and kidney transplantation by testing for immediate early antigenemia and influence of cellular immunity on the occurrence of CMV infection. Journal of Clinical Microbiology 28:2069–2075
    [Google Scholar]
  4. Boom R. 1987 Repression and activation of the human cytomegalovirus major immediate early transcription unit Ph,. D. thesis University of Amsterdam;
    [Google Scholar]
  5. Boom R., Geelen J. L., Sol C. J., Raap A. K., Minnaar R. P., Klaver B. P., van der Noordaa J. 1986; Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate-early gene products by protein synthesis inhibition. Journal of Virology 58:851–859
    [Google Scholar]
  6. Britt W. J., Vugler L. 1987; Structural and immunological characterization of the intracellular forms of an abundant 68000 Mr human cytomegalovirus protein. Journal of General Virology 68:1897–1907
    [Google Scholar]
  7. Cranage M. P., Smith G. L., Bell S. E., Hart H., Brown C., Bankier A. T., Tomlinson P., Barrell B. G., Minson T. C. 1988; Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein–Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 glycoprotein H. Journal of Virology 62:1416–1422
    [Google Scholar]
  8. Dankner W. M., McCutchan J. A., Richman D. D., Hirata K., Spector S. A. 1990; Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization. Journal of Infectious Diseases 161:31–36
    [Google Scholar]
  9. Depto A. S., Stenberg R. M. 1989; Regulated expression of the human cytomegalovirus pp65 gene: octamer sequence in the promotor is required for activation by viral gene products. Journal of Virology 63:1232–1238
    [Google Scholar]
  10. Felgner P. L., Gadek T. R., Holm M., Roman R., Chan H. W., Wenz M., Northrop J. P., Ringold G. M., Danielsen M. 1987; Lipofectin: a highly efficient, lipid-mediated DNA-transfection procedure. Proceedings of the National Academy of Sciences, U. S. A. 84:7413–7417
    [Google Scholar]
  11. Geballe A. P., Leach F. S., Mocarski E. S. 1986; Regulation of cytomegalovirus late gene expression: gamma genes are controlled by post-transcriptional events. Journal of Virology 57:864–874
    [Google Scholar]
  12. Gerna G., Revello M. G., Percivalle E., Zavattoni M., Parea M., Battaglia M. 1990; Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. Journal of Clinical Microbiology 28:2681–2688
    [Google Scholar]
  13. Hersman J., Meyers J. D., Thomas E. D., Buckner C. D., Clift R. 1982; The effect of granulocyte transfusions on the incidence of cytomegalovirus infection after allogeneic marrow transplantation. Annals of Internal Medicine 96:149–152
    [Google Scholar]
  14. Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. 1990 PCR Protocols: A Guide to Methods and Applications San Diego: Academic Press;
    [Google Scholar]
  15. Irmiere A., Gibson W. 1983; Isolation and characterization of a non-infectious virion-like particle released from cells infected with human strains of cytomegalovirus. Virology 130:118–133
    [Google Scholar]
  16. Jahn G., Scholl B. C., Traupe B., Fleckenstein B. 1987; The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties. Journal of General Virology 68:1327–1337
    [Google Scholar]
  17. Jiwa N. M., Van Gemert G. W., Raap A. K., Van de Rijke F. M., Mulder A., Lens P. F., Salimans M. M. M., Zwaan F. E., Van Dorp W., Van Der Ploeg M. 1989; Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction. Transplantation 48:12–76
    [Google Scholar]
  18. Klages S., Rüger B., Jahn G. 1989; Multiplicity dependent expression of the predominant phosphoprotein pp65 of human cytomegalovirus. Virus Research 12:159–168
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. LaFemina R. L., Pizzorno M. C., Mosca J. D., Hayward G. S. 1989; Expression of the acidic nuclear immediate-early protein (IE1) of human cytomegalovirus in stable cell lines and its preferential association with metaphase chromosomes. Virology 172:584–600
    [Google Scholar]
  21. Landini M. P., Severi B., Furlini G., Badiali de Giorgi L. 1987; Human cytomegalovirus structural components: intracellular and intraviral localization of p28 and p65–69 by immunoelectron microscopy. Virus Research 8:15–23
    [Google Scholar]
  22. Lautenschlager I., Ahonen J., Eklund B., Hoeckerstedt K., Isoniemi H., Salmela K., Hayri P. 1990; Detection of cytomegalovirus-specific early antigens from blood leukocytes and bronchoalveolar lavage cells in the diagnosis of active cytomegalovirus infection in transplant patients. Transplantation Proceedings 22:233
    [Google Scholar]
  23. Lindenmaier W., Necker A., Krause S., Bonewald R., Collins J. 1990; Cloning and characterization of major antigenic determinants of human cytomegalovirus Ad 169 seen by the human immune system. Archives of Virology 113:1–16
    [Google Scholar]
  24. Marsh J. L., Erfle J., Wykes E. J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481–485
    [Google Scholar]
  25. Mazeron M. -C., Berbar T., Guillemin M. -C., Colimon R., Roseto R., Perol Y. 1983; Production d’anticorps monoclonaux contre le cytomegalovirus humain. Compte rendu des seances de l’ Académie des sciences 297:305–308
    [Google Scholar]
  26. Michelson S., Tardy-Panit M., Barzu O. 1984; Properties of a human cytomegalovirus-induced protein kinase. Virology 134:259–268
    [Google Scholar]
  27. Middeldorp J. M., Jongsma J., The T. H. 1984; Detection of immunoglobulin M and G antibodies against cytomegalovirus early and late antigens by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 20:763–771
    [Google Scholar]
  28. Middeldorp J. M., Jongsma J., The T. H. 1986; Immunofluorescence for detection of antibodies against human cytomegalovirus-induced membrane antigens. Journal of Clinical Microbiology 24:405–413
    [Google Scholar]
  29. Niedobitek G., Finn T., Herbst H., Gerdes J., Grillner L., Landqvist M., Zweygberg Wirgart B., Stein H. 1988; Detection of cytomegalovirus by in situ hybridization and immuno-histochemistry using new monoclonal antibody CCH2: a comparison of methods. Journal of Clinical Pathology 41:1005–1009
    [Google Scholar]
  30. Pande H., Lee T. D., Churchill M. A., Zaia J. A. 1990; Structural analysis of a 64-kDa major structural protein of human cytomegalovirus (Towne): identification of a phosphorylation site and comparison to pp65 of HCMV (AD 169). Virology 178:6–14
    [Google Scholar]
  31. Plachter B., Klages S., Hagelmann S., Britt W., Landini M. P., Jahn G. 1990; Procaryotic expression of phosphorylated tegument protein pp65 of human cytomegalovirus and application of recombinant peptides for immunoblot analysis. Journal of Clinical Microbiology 28:1229–1235
    [Google Scholar]
  32. Revello M. G., Percivalle E., Zavattoni M., Parea M., Grossi P., Gerna G. 1989; Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients. Journal of Medical Virology 29:8893
    [Google Scholar]
  33. Revello M. G., Percivalle E., Di Matteo A., Morini F., Gerna G. 1992; Nuclear expression of the lower matrix protein of human cytomegalovirus in peripheral blood leukocytes of immunocompromised viraemic patients. Journal of General Virology 73:437–442
    [Google Scholar]
  34. Rinaldo C. R., Black P. H., Hirsch M. S. 1977; Interaction of cytomegalovirus with leukocytes from patients with mononucleosis due to cytomegalovirus. Journal of Infectious Diseases 136:667–678
    [Google Scholar]
  35. Roby C., Gibson W. 1986; Characterization of phosphoproteins and protein kinase activity of virions, noninfectious enveloped particles, and dense bodies of human cytomegalovirus. Journal of Virology 59:714–727
    [Google Scholar]
  36. Rüger B., Klages S., Walla B., Albrecht J., Fleckenstein B., Tomlinson P., Barrell B. 1987; Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus. Journal of Virology 61:446–453
    [Google Scholar]
  37. Saltzman R. L., Quirk M. R., Jordan M. C. 1988; Disseminated cytomegalovirus infection: molecular analysis of virus and leukocyte interactions in viremia. Journal of Clinical Investigation 81:75–81
    [Google Scholar]
  38. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  39. Schrier R. D., Nelson J. A., Oldstone M. B. A. 1985; Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. Science 230:1048–1051
    [Google Scholar]
  40. Shuster E. A., Beneke J. S., Tegtmeier G. E., Pearson G. R., Gleaves C. A., Wold A. D., Smith T. F. 1985; Monoclonal antibodies for rapid laboratory detection of cytomegalovirus infections: characterization and diagnostic application. Mayo Clinic Proceedings 60:577–585
    [Google Scholar]
  41. Somogyi T., Michelson S., Masse M. O. 1990; Genomic location of a human cytomegalovirus protein with protein kinase activity (PK68). Virology 174:276–285
    [Google Scholar]
  42. Stinski M. F., Thomsen D. R., Stenberg R. M., Goldstein L. C. 1983; Organization and expression of the immediate early genes of human cytomegalovirus. Journal of Virology 46:1–14
    [Google Scholar]
  43. The T. H., van der Bij W., van Den Berg A. P. M., van der Giessen M., Weits J., Sprenger H. G., van Son W. J. 1990; Cytomegalovirus antigenemia. Reviews of Infectious Diseases 12:S737–S744
    [Google Scholar]
  44. van den Berg A. P., van der Bii W., van Son W. J., Anema J., van der Giessen M., Schirm J., Tegzess A. M., The T. H. 1989; Cytomegalovirus-antigenemia as a useful marker of symptomatic cytomegalovirus infection after renal transplantation: a report of 130 consecutive patients. Transplantation 48:991–995
    [Google Scholar]
  45. van den Berg A. P., Klompmaker I. J., Haagsma E. B., Scholten-Sampson A., Bijleveld C. M. A., Schirm J., van der Giessen M., Slooff M. J. H., The T. H. 1991; Antigenemia in the diagnosis and monitoring of active cytomegalovirus infection after liver transplantation. Journal of Infectious Diseases 164:265–270
    [Google Scholar]
  46. van der Bij W., Schirm J., Torensma R., van Son W. J., Tegzess A. M., The T. H. 1988a; Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. Journal of Clinical Microbiology 26:2531–2535
    [Google Scholar]
  47. van der Bij W., Torensma R., van Son W. J., Anema J., Schirm J., Tegzess A. M., The T. H. 1988b; Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leukocytes. Journal of Medical Virology 25:179–188
    [Google Scholar]
  48. van der Voort L. H. M., van zanten J., De Leij L. F. M. H., The T. H. 1989; Determination of human antibodies to specific antigens of cytomegalovirus using an antigen capture immunoassay. Journal of Immunological Methods 121:95–103
    [Google Scholar]
  49. Wathen M. W., Thomsen D. R., Stinski M. F. 1981; Temporal regulation of human cytomegalovirus transcription at immediate early and early times after infection. Journal of Virology 38:446–459
    [Google Scholar]
  50. Weiner D., Gibson W., Fields K. L. 1985; Anti-complement immunofluorescence establishes nuclear localization of human cytomegalovirus matrix protein. Virology 147:19–28
    [Google Scholar]
  51. Winston D. J., Ho W. D., Howell C. L., Miller M. J., Mickey R., Martin W. J., Lin C., Gale R. P. 1980; Cytomegalovirus infections associated with leukocyte transfusions. Annals of Internal Medicine 93:671–675
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-11-2923
Loading
/content/journal/jgv/10.1099/0022-1317-73-11-2923
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error