1887

Abstract

Infectious pancreatic necrosis virus (IPNV) is the aetiological agent of an important disease in hatchery-reared salmonid fish in North America, Europe and Japan. It belongs to the family Birnaviridae and shows a high degree of antigenic heterogeneity. However, genomic variations between the 10 identified serotypes have not yet been studied. In order to correlate genomic heterogeneity with the different serotypes, oligonucleotides were synthesized according to the published sequence of the Jasper strain (serotype A9). They were used as primers for the amplification of a 359 bp cDNA fragment of the viral genome using the polymerase chain reaction. Fragments amplified from 37 strains were digested with five different restriction enzymes. Restriction fragment profiles obtained on agarose gels showed heterogeneity not only between strains of different serotypes, but also among those belonging to serotype A1. A cluster analysis of the restriction patterns showed that IPNV strains can be divided into three major groups, corresponding approximately to serotypes A1, A2 and A3, and 10 subgroups which do not correlate with the serotyping of the strains.

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1992-11-01
2021-10-20
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References

  1. Ahne W. 1981; Serological techniques currently used in fish virology. Developments in Biological Standardization 49:3–27
    [Google Scholar]
  2. Azad A. A., Jagadish M. N., Brown M. A., Hudson P. J. 1987; Deletion mapping and expression in Escherichia coli of the large genomic segment of a bimavirus. Virology 161:145–152
    [Google Scholar]
  3. Bayliss C. D., Spies U., Shaw K., Peters R. W., Papageorgiou A., Müller H., Boursnell M. E. G. 1990; A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. Journal of General Virology 71:1303–1312
    [Google Scholar]
  4. Becht H., Muller H., Muller H. K. 1988; Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. Journal of General Virology 69:631–640
    [Google Scholar]
  5. Calvert J. G., Nagy E., Soler M., Dobos P. 1991; Characterization of the VPg–dsRNA linkage of infectious pancreatic necrosis virus. Journal of General Virology 72:2563–2567
    [Google Scholar]
  6. Caswell-Reno P., Reno P. W., Nicholson B. L. 1986; Monoclonal antibodies to infectious pancreatic necrosis virus: analysis of viral epitopes and comparison of different isolates. Journal of General Virology 67:2193–2205
    [Google Scholar]
  7. Caswell-Reno P., Lipipun V., Reno P. W., Nicholson B. L. 1989; Use of a group-reactive and other monoclonal antibodies in an enzyme immunodot assay for identification and presumptive serotyping of aquatic birnaviruses. Journal of Clinical Microbiology 27:1924–1929
    [Google Scholar]
  8. Christie K. E., Håvarstein L. S., Djupvik H. O., Ness S., Endresen C. 1988; Characterization of a new serotype of infectious pancreatic necrosis virus isolated from Atlantic salmon. Archives of Virology 103:167–177
    [Google Scholar]
  9. Christie K. E., Ness S., Djupvik H. O. 1990; Infectious pancreatic necrosis virus in Norway: partial serotyping by monoclonal antibodies. Journal of Fish Diseases 13:323–327
    [Google Scholar]
  10. Dobos P. 1977; Virus-specific protein synthesis in cells infected by infectious pancreatic necrosis virus. Journal of Virology 21:242–258
    [Google Scholar]
  11. Dobos P., Roberts T. E. 1983; The molecular biology of infectious pancreatic necrosis virus: a review. Canadian Journal of Microbiology 29:377–384
    [Google Scholar]
  12. Dobos P., Rowe D. 1977; Peptide map comparison of infectious pancreatic necrosis virus-specific polypeptides. Journal of Virology 24:805–820
    [Google Scholar]
  13. Dominguez J., Babin M., Sanchez C., Hedrick R. P. 1991; Rapid serotyping of infectious pancreatic necrosis virus by one-step enzyme-linked immunosorbent assay using monoclonal antibodies. Journal of Virological Methods 31:93–104
    [Google Scholar]
  14. Dorson M., Torchy C. 1981; The influence of fish age and water temperature on mortalities of rainbow trout, Salmo gairdneri Richardson, caused by a European strain of infectious pancreatic necrosis virus. Journal of Fish Diseases 4:213–221
    [Google Scholar]
  15. Duncan R., Dobos P. 1986; The nucleotide sequence of infectious pancreatic necrosis virus (1PNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein. Nucleic Acids Research 14:5934
    [Google Scholar]
  16. Duncan R., Nagy E., Krell P. J., Dobos P. 1987; Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of a virus-encoded protease, and fine structure mapping of genome segment A coding regions. Journal of Virology 61:3655–3664
    [Google Scholar]
  17. Duncan R., Mason C. L., Nagy E., Leong J., Dobos P. 1991; Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif. Virology 181:541–552
    [Google Scholar]
  18. Fahey K. J., Erny K., Crooks J. 1989; A conformational immunogen on VP-2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens. Journal of General Virology 70:1473–1481
    [Google Scholar]
  19. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Fifth report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2
    [Google Scholar]
  20. Frantsi C., Savan M. 1971; Infectious pancreatic necrosis virus - temperature and age factors in mortality. Journal of Wildlife Diseases 7:249–255
    [Google Scholar]
  21. Håvarstein L. S., Kalland K. H., Christie K. E., Endresen C. 1990; Sequence of the large double-stranded RNA segment of the N1 strain of infectious pancreatic necrosis virus: a comparison with other Birnaviridae . Journal of General Virology 71:299–308
    [Google Scholar]
  22. Hill B., Way K. 1983; Serological classification of fish and shellfish birnaviruses. Abstract First International Conference of the European Association of Fish PathologistsPlymouth, U.K. p 10
    [Google Scholar]
  23. Hill B. J., Way K. 1988; Proposed standardization of the serological classification of aquatic birnaviruses. Abstract International Fish Health ConferenceVancouver, Canada Conference handbook p 151
    [Google Scholar]
  24. Hsu Y.-L., Chen B.-S., Wu J.-L. 1989; Comparison of RNAs and polypeptides of infectious pancreatic necrosis virus isolates from eel and rainbow trout. Journal of General Virology 70:2233–2239
    [Google Scholar]
  25. Jagadish M. N., Azad A. A. 1991; Localization of a VP3 epitope of infectious bursal disease virus. Virology 184:805–807
    [Google Scholar]
  26. Jørgensen P. E. V., Kehlet N. P. 1971; Infectious pancreatic necrosis (IPN) viruses in Danish rainbow trout: their serological and pathogenic properties. Nordisk Veterinaermedicin 23:568–575
    [Google Scholar]
  27. Kibenge F. S. B., Jackwood D. J., Mercado C. C. 1990; Nucleotide sequence analysis of genome segment A of infectious bursal disease virus. Journal of General Virology 71:569–577
    [Google Scholar]
  28. Kibenge F. S. B., McKenna P. K., Dybing J. K. 1991; Genome cloning and analysis of the large RNA segment (segment A) of a naturally avirulent serotype 2 infectious bursal disease virus. Virology 184:437–440
    [Google Scholar]
  29. Lapierre J., Larrivée D., Berthiaume L. 1986; Influence of water temperature and fish age on mortality in brook trout (Salve/inus fontinalis) infected with infectious pancreatic necrosis virus (IPNV). Aquaculture 59:81–92
    [Google Scholar]
  30. Lecomte J., Arella M., Berthiaume L. 1992; Comparison of polyclonal and monoclonal antibodies for serotyping infectious pancreatic necrosis virus (IPNV) strains isolated in Quebec and Newfoundland. Journal of Fish Diseases 15: (in press)
    [Google Scholar]
  31. Lipipun V., Caswell-Reno P., Hsu Y. L., Wu J. L., Tung M. C., Reno P. W., Wattanavijarn W., Nicholson B. L. 1989; Antigenic analysis of Asian aquatic bimavirus isolates using monoclonal antibodies. Fish Pathology 24:155–160
    [Google Scholar]
  32. MacDonald R. D., Dobos P. 1981; Identification of the protein encoded by each genome segment of infectious pancreatic necrosis virus. Virology 114:414–422
    [Google Scholar]
  33. MacDonald R. D., Gower D. A. 1981; Genomic and phenotypic divergence among three serotypes of aquatic birnaviruses (infectious pancreatic necrosis virus). Virology 114:187–195
    [Google Scholar]
  34. McMichael J., Fryer J. L., Pilcher K. S. 1978; An antigenic comparison of three strains of infectious pancreatic necrosis virus of salmonid fishes. Aquaculture 6:203–210
    [Google Scholar]
  35. Nagy E., Dobos P. 1987; Epitope mapping of infectious pancreatic necrosis virus (IPNV) polypeptides using monoclonal antibodies. Abstract VII International Congress of Virology Edmonton, Canada: p 311
    [Google Scholar]
  36. Nicholson B. L., Pochebit S. 1981; Antigenic analysis of infectious pancreatic necrosis virus (IPNV) by neutralization kinetics. Developments in Biological Standardization 49:35–41
    [Google Scholar]
  37. Okamoto N., Sano T., Hedrick R. P., Fryer J. L. 1983; Antigenic relationships of selected strains of infectious pancreatic necrosis virus and European eel virus. Journal of Fish Diseases 6:19–25
    [Google Scholar]
  38. Persson R. H., MacDonald R. D. 1982; Evidence that infectious pancreatic necrosis virus has a genome linked protein. Journal of Virology 44:437–443
    [Google Scholar]
  39. Pilcher K. S., Fryer J. L. 1980; The viral diseases of fish: a review through 1978. CRC Critical Reviews in Microbiology 7:287–364
    [Google Scholar]
  40. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  41. Wolf K., Quimby M. C. 1971; Salmonid viruses: infectious pancreatic necrosis virus. Morphology, pathology and serology of first European isolations. Archiv fur die gesamte Virusforschung 34:144–156
    [Google Scholar]
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