1887

Abstract

A viroid was isolated from symptomless plants using the return-PAGE method for analysis of low nucleic acids. The RNA was transmitted to tomato, three cultivars of potato, and plants by mechanical inoculation or by grafting. Infected solanaceous plants developed symptoms similar to those caused by potato spindle tuber viroid (PSTVd). The viroid consists of 372 nucleotides, 214 G+C, 158 A+U, with a G+C/A+U ratio of 1.35. One of seven cDNA clones showed a sequence heterogeneity (G to A) at position 73. The most stable secondary structure of this viroid has 78 G:C, 37 A:U and 11 G:U base pairs with a minimum free energy of -456.9 kJ. The viroid is closely related to the 370 nucleotide latent viroid. The viroid possesses regions of 100% sequence identity with six viroids belonging to the PSTVd and apple scar skin viroid groups. The viroid also replicated in tomato plants when mixed with PSTVd. Tomato plants were cross-protected against PSTVd when preinfected with the viroid from .

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1992-11-01
2022-01-27
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References

  1. Gross H. J., Liebl U., Alberty H., Krupp G., Ramm K., Sänger H. L. 1981; A severe and a mild potato spindle tuber viroid isolate differ in three nucleotide exchanges only. Bioscience Reports 1:235–241
    [Google Scholar]
  2. Gross H. J., Krupp G., Domedy H., Raba M., Jank P., Lossow C., Alberty H., Ramm K., Sänger H. L. 1982; Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid. European Journal of Biochemistry 121:249–257
    [Google Scholar]
  3. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  4. Hammond R., Smith D. R., Diener T. O. 1989; Nucleotide sequence and proposed secondary structure of Columnea latent viroid: a natural mosaic of viroid sequences. Nucleic Acids Research 23:10083–10094
    [Google Scholar]
  5. Harold T., Haas B., Singh R. P., Boucher A., Sänger H. L. 1992; Sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (PSTVd) is not strictly conserved but as variable as in other viroids. Plant Molecular Biology 19:329–333
    [Google Scholar]
  6. Haseloff J., Symons R. H. 1981; Chrysanthemum stunt viroid: primary and secondary structure. Nucleic Acids Research 9:2741–2752
    [Google Scholar]
  7. Hashimoto J., Koganezawa H. 1987; Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Research 15:7045–7051
    [Google Scholar]
  8. Keese P., Symons R. H. 1985; Domains in viroids: evidence of intermolecular rearrangements and their contribution to viroid evolution. Proceedings of the National Academy of Sciences, U. S. A. 82:4582–4586
    [Google Scholar]
  9. Kiefer M. C., Owens R. A., Diener T. O. 1983; Structural similarities between viroids and transposable genetic elements. Proceedings of the National Academy of Sciences, U. S. A. 80:6234–6238
    [Google Scholar]
  10. Koltunow A. M., Rezaian M. A. 1989; A scheme of viroid classification. Intervirology 30:194–201
    [Google Scholar]
  11. Lakshman D. K., Tavantzis S. M. 1992; RNA progeny from an infectious two-base deletion cDNA mutant of potato spindle tuber viroid (PSTV) acquire two nucleotides in planta . Virology 187:565–572
    [Google Scholar]
  12. Lakshman D. K., Tavantzis S. M., Boucher A., Singh R. P. 1992; A rapid and versatile method for cloning viroid or other circular plant pathogenic RNA’s. Analytical Biochemistry 203:269–273
    [Google Scholar]
  13. Lee J. Y., Puchta H., Ramm K., Sänger H. L. 1988; Nucleotide sequence of the Korean strain of hop stunt viroid. Nucleic Acids Research 16:8708
    [Google Scholar]
  14. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U. S. A. 74:5463–5467
    [Google Scholar]
  16. Singh R. P. 1983; Viroids and their potential danger to potatoes in hot climates. Canadian Plant Disease Survey 63:13–18
    [Google Scholar]
  17. Singh R. P., Boucher A. 1987; Electrophoretic separation of a severe strain from mild strains of potato spindle tuber viroid. Phytopathology 77:1588–1591
    [Google Scholar]
  18. Singh R. P., Crowley C. F. 1985; Evaluation of polyacrylamide gel electrophoresis, bioassay, and dot-blot methods for the survey of potato spindle tuber viroid. Canadian Plant Disease Survey 65:61–63
    [Google Scholar]
  19. Singh R. P., Dehaan T.-L., Jaswal A. S. 1988; A survey of the incidence of potato spindle tuber viroid in Prince Edward Island using two testing methods. Canadian Journal of Plant Science 68:1229–1236
    [Google Scholar]
  20. Singh R. P., Boucher A., Seabrook J. E. A. 1988; Detection of the mild strains of potato spindle tuber viroid from single true potato seed by return electrophoresis. Phytopathology 78:663–667
    [Google Scholar]
  21. Singh R. P., Boucher A., Singh A. 1991; High incidence of transmission and occurrence of a viroid in commercial seeds of Coleus in Canada. Plant Disease 75:184–187
    [Google Scholar]
  22. Singh R. P., Khoury J., Boucher A., Somerville T. H. 1989; Characteristics of cross-protection with potato spindle tuber viroid strains in tomato plants. Canadian Journal of Plant Pathology 11:263–267
    [Google Scholar]
  23. Spieker R. L., Haas B., Charng Y., Freimüller K., Sänger H. L. 1990; Primary and secondary structure of a new viroid ‘species’ (CbVd 1) present in the Coleus blumei cultivar ‘Bienvenue’. Nucleic Acids Research 18:3998
    [Google Scholar]
  24. Zuker M. 1989; On finding all suboptimal foldings of an RNA molecule. Science 244:48–52
    [Google Scholar]
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