1887

Abstract

A set of full-length cDNA clones of the satellite RNA of grapevine fanleaf nepovirus isolate F13 (GFLV-F13) was constructed with a variable number of additional, non-viral nucleotides at the 5′ and 3′ ends. The biological activity of the RNAs transcribed from these constructs was tested in protoplasts using a helper virus. When inoculated with arabis mosaic virus S (ArMV-S) RNA as helper, transcripts with 33 non-viral nucleotides at the 5′ end (tr45p4) did not replicate, whereas transcripts with only one non-viral nucleotide at the 5′ end (tr3S and tr3M) did replicate. Capping of the transcripts enhanced their replication. On the other hand, the presence of extra nucleotides at the 3′ end had little influence on the biological activity of the transcripts. In contrast with ArMV-S, GFLV isolate 24 was not a helper for tr3M transcripts, indicating a specific interaction between the helper strain and the satellite RNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-10-2517
1992-10-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/10/JV0730102517.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-10-2517&mimeType=html&fmt=ahah

References

  1. Dawson W. O., Beck D. L., Knorr D. A., Grantham G. L. 1986; cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proceedings of the National Academy of Sciences, U.S.A. 83:1832–1836
    [Google Scholar]
  2. Demangeat G., Greif C., Hemmer O., Fritsch C. 1990; Analysis of the in vitro cleavage products of the tomato black ring virus RNA-l-encoded 250K polyprotein. Journal of General Virology 71:1649–1654
    [Google Scholar]
  3. de Varennes A., Russo M., Maule A. J. 1984; Infection of protoplasts from Chenopodium quinoa with cowpea mosaic and cymbidium ringspot viruses. Journal of General Virology 65:1851–1855
    [Google Scholar]
  4. Domier L. L., Franklin K. M., Hunt A. G., Rhoads R. E. 1989; Infectious in vitro transcripts from cloned cDNA of a potyvirus, tobacco vein mottling virus. Proceedings of the National Academy of Sciences, U.S.A. 86:3509–3513
    [Google Scholar]
  5. Dore J.-M., Pinck M., Pinck L. 1989; Competitive multiplication of RNA3 species of different strains of alfalfa mosaic virus. Journal of General Virology 70:777–782
    [Google Scholar]
  6. Eggen R., Verver J., Wellink J., de Jong A., Goldbach R., van Kammen A. 1989; Improvements of the infectivity of in vitro transcripts from cloned cowpea mosaic virus cDNA: impact of terminal nucleotide sequences. Virology 173:447–455
    [Google Scholar]
  7. Fritsch C., Mayo M. A. 1989; Satellites of plant viruses. In Plant Viruses vol 1 pp. 289–321 Edited by Mandahar C. L. Boca Raton: CRC Press;
    [Google Scholar]
  8. Fuchs M. 1989 Le grapevine fanleaf virus, agent du court-noué de la vigne: approche moléculaire de la prémunition, structure et expression du RNA satellite D. Phil. thesis Université Louis Pasteur, Strasbourg;
    [Google Scholar]
  9. Fuchs M., Pinck M., Serghini M. A., Ravelonandro M., Walter B., Pinck L. 1989; The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13. Journal of General Virology 70:955–962
    [Google Scholar]
  10. Fuchs M., Pinck M., Etienne L., Pinck L., Walter B. 1991; Characterization and detection of grapevine fanleaf virus by using cDNA probes. Phytopathology 81:559–565
    [Google Scholar]
  11. Godefroy-Colburn T., Thivent C., Pinck L. 1985; Translational discrimination between the four RNAs of alfalfa mosaic virus. A quantitative evaluation. European Journal of Biochemistry 147:541–548
    [Google Scholar]
  12. Greif C., Hemmer O., Demangeat G., Fritsch C. 1990; In vitro synthesis of biologically active transcripts of tomato black ring virus satellite RNA. Journal of General Virology 71:907–915
    [Google Scholar]
  13. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:357–380
    [Google Scholar]
  14. Heaton L. A., Carrington J. C., Morris T. J. 1989; Turnip crinkle virus infection from RNA synthesized in vitro . Virology 170:214–218
    [Google Scholar]
  15. Hemmer O., Meyer M., Greif C., Fritsch C. 1987; Comparison of the nucleotide sequences of five tomato black ring virus satellite RNAs. Journal of General Virology 68:1823–1833
    [Google Scholar]
  16. Janda M., French R., Ahlquist P. 1987; High efficiency T7 polymerase synthesis of infectious RNA from cloned brome mosaic virus cDNA and effects of 5′ extensions on transcript infectivity. Virology 158:259–262
    [Google Scholar]
  17. Kunkel T. A. 1985; Rapid and efficient site-specific mutagenesis without phenotypic selection. Proceedings of the National Academy of Sciences, U.S.A. 82:488–492
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  19. Liu Y. Y., Hellen C. U. T., Cooper J. I., Bertioli D. J., Coates D., Bauer G. 1990; The nucleotide sequence of a satellite RNA associated with arabis mosaic nepovirus. Journal of General Virology 71:1259–1263
    [Google Scholar]
  20. Liu Y. Y., Cooper J. I., Coates D., Bauer G. 1991; Biologically active transcripts of a large satellite RNA from arabis mosaic nepovirus and the importance of 5′ end sequences for its replication. Journal of General Virology 72:2867–2874
    [Google Scholar]
  21. Margis R., Viry M., Pinck M., Pinck L. 1991; Cloning and in vitro characterization of the grapevine fanleaf virus proteinase cistron. Virology 185:779–787
    [Google Scholar]
  22. Masuta C., Kuwata S., Takanami Y. 1988; Effect of extra 5′ non-viral bases on the infectivity of transcripts from a cDNA clone of satellite RNA (strain Y) of cucumber mosaic virus. Journal of Biochemistry 104:841–846
    [Google Scholar]
  23. Murant A. F., Mayo M. A. 1982; Satellites of plant viruses. Annual Review of Phytopathology 20:49–70
    [Google Scholar]
  24. Pinck L., Fuchs M., Pinck M., Ravelonandro M., Walter B. 1988; A satellite RNA in grapevine fanleaf virus strain F13. Journal of General Virology 69:233–239
    [Google Scholar]
  25. Pinck M., Reinbolt J., Loudes A. M., Le Ret M., Pinck L. 1991; Primary structure and location of the genome-linked protein (VPg) of grapevine fanleaf virus. FEBS Letters 284:117–119
    [Google Scholar]
  26. Riechmann J. L., LaÍn S., GarcÍa J. A. 1990; Infectious in vitro transcripts from plum pox potyvirus cDNA clone. Virology 177:710–716
    [Google Scholar]
  27. Ritzenthaler C., Viry M., Pinck M., Margis R., Fuchs M., Pinck L. 1991; Complete nucleotide sequence and genetic organization of grapevine fanleaf nepovirus RNA1. Journal of General Virology 72:2357–2365
    [Google Scholar]
  28. Roossinck M. J., Palukaitis P. 1991; Differential replication in zucchini squash of cucumber mosaic virus satellite RNA maps to RNA1 of the helper virus. Virology 181:371–373
    [Google Scholar]
  29. Rottier P. J. M., Rezelman G., van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  30. Rubino L., Tousignant M. E., Steger G., Kaper J. M. 1990; Nucleotide sequence and structural analysis of two satellite RNAs associated with chicory yellow mottle virus. Journal of General Virology 71:1897–1903
    [Google Scholar]
  31. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  32. Serghini M. A., Fuchs M., Pinck M., Reinbolt J., Walter B., Pinck L. 1990; RNA2 of grapevine fanleaf virus: sequence analysis and coat protein cistron location. Journal of General Virology 71:1433–1441
    [Google Scholar]
  33. Shimotohno K., Kodama Y., Hashimoto J., Miura K. I. 1977; Importance of 5′-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis. Proceedings of the National Academy of Sciences, U.S.A. 74:2734–2738
    [Google Scholar]
  34. Valverde R. A., Heick J. A., Dodds A. 1991; Interactions between satellite tobacco mosaic virus, helper tobamovirus, and their hosts. Phytopathology 81:99–104
    [Google Scholar]
  35. VAN DER WERF S., Bradley J., Wimmer E., Studier F. W., Dunn J. J. 1986; Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proceedings of the National Academy of Sciences, U.S.A. 83:2330–2334
    [Google Scholar]
  36. Veidt I., Bouzoubaa S. E., Leiser R. M., Ziegler-Graff V., Guilley H., Richards K., Jonard G. 1992; Synthesis of full-length transcripts of beet western yellows virus RNA: messenger properties and biological activity in protoplasts. Virology 186:192–200
    [Google Scholar]
  37. Vos P., Jaegle M., Wellink J., Verver J., Eggen R., van Kammen A., Goldbach R. 1988; Infectious RNA transcripts derived from full-length DNA copies of the genomic RNAs of cowpea mosaic virus. Virology 165:33–41
    [Google Scholar]
  38. Widholm J. M. 1972; The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technology 47:189–194
    [Google Scholar]
  39. Young M. J., Kelly L., Larkin P. J., Waterhouse P. M., Gerlach W. L. 1991; Infectious in vitro transcripts from a cloned cDNA of barley yellow dwarf virus. Virology 180:372–379
    [Google Scholar]
  40. Ziegler-Graff V., Bouzoubaa S., Jupin I., Guilley H., Jonard G., Richards K. 1988; Biologically active transcripts of beet necrotic yellow vein virus RNA-3 and RNA-4. Journal of General Virology 69:2347–2357
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-10-2517
Loading
/content/journal/jgv/10.1099/0022-1317-73-10-2517
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error