1887

Abstract

Under conditions in which a clonal cell line (M10) isolated from a human T cell lymphotrophic virus type I-transformed MT-4 cell line was completely killed by infection with wild-type human immunodeficiency virus type 1 (HIV-1), equivalent M10 cells survived infection with HIV-1 or mutant virus after transient cytopathic effects. Several cell clones, which were isolated from the proliferating M10 cells after infection with and mutant viruses (M10/ and M10/ ), had heterogeneous HIV-1 phenotypes in terms of HIV-1 antigen expression, their syncytium forming capacity, reverse transcriptase activity and the infectivity of HIV-1 particles produced. When the replication kinetics of the HIV-1 particles produced were assayed in M10 cells, the clones could be classified into three types, i.e. type I producing non-infectious HIV-1, type II producing infectious HIV-1 with low replicative ability and type III producing infectious HIV-1 with a replicative ability similar to that of wild-type HIV-1. HIV-1 major viral cell proteins and virus particle fractions were almost typical in types II and III but not in type I. Electron microscopic examination of particles released by I, II and III clones revealed rare defective, predominantly defective and essentially normal virions, respectively. Northern and Southern blot analyses revealed no apparent deletion in the proviral DNA and mRNA prepared from these clones, except in the case of type I and II clones isolated from M10/ which contained large deletions in the mRNAs for and proteins. Thus, M10 cells surviving infection with HIV-1 or mutants are heterogeneous, persistently expressing HIV-1 antigens and producing non-infectious or less cytopathic virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-1-77
1992-01-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/1/JV0730010077.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-1-77&mimeType=html&fmt=ahah

References

  1. Adachi A., Gendelman H. E., Koenig S., Folks T., Willey R., Robson A., Martin M. A. 1986; Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. Journal of Virology 59:284–291
    [Google Scholar]
  2. Adachi A., Koenig S., Gendelman H. E., Daugherty D., Gattoni-Celli S., Fauci A. S., Martin M. A. 1987; Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. Journal of Virology 61:209–213
    [Google Scholar]
  3. Adachi A., Ono N., Sakai H., Ogawa K., Shibata R., Kiyomasu T., Masuike H., Ueda S. 1991; Generation and characterization of the human immunodeficiency virus type 1 mutants. Archives of Virology 117:45–58
    [Google Scholar]
  4. Alizon M., Wain-Hobson S., Montagnier L., Sonigo P. 1986; Genetic variability of the AIDS virus. Cell 46:63–74
    [Google Scholar]
  5. Allain J.-P., Laurian Y., Paul D. A., Senn D. 1986; Serological markers in the early stages of human immunodeficiency virus infection in haemophiliacs. Lancet ii:1233–1236
    [Google Scholar]
  6. Åsjö B., Albert J., Karlsson A., Morfeldt-Mamson L., Biberfeld G., Lidman K., Fenyo E. M. 1986; Replicative properties of human immunodeficiency virus from patients with varying severity of HIV infection. Lancet ii:660–662
    [Google Scholar]
  7. Bacchetti P., Moss A. R. 1989; Incubation period of AIDS in San Francisco. Nature, London 338:251–253
    [Google Scholar]
  8. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vésinet-Brun F., Rozioux C., Rozenbaum W., Montagnier L. 1983; Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science 220:868–871
    [Google Scholar]
  9. Benn S., Rutledge R., Folks T., Gold J., Baker L., McCormick J., Feorino P., Piot P., Guinn T., Martin M. 1985; Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire. Science 230:1548–1553
    [Google Scholar]
  10. Cheng-Mayer C., Quiroga M., Tung J. W., Dina D., Levy J. A. 1990; Viral determinants of human immunodeficiency virus type 1 T-cell or macrophage tropism, cytopathogenicity, and CD4 antigen modulation. Journal of Virology 64:4390–4398
    [Google Scholar]
  11. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  12. Engelke D. R., Hoener P. A., Collins F. S. 1988; Direct sequencing of enzymatically amplified human genomic DNA. Proceedings of the National Academy of Sciences, U.S.A 85:544–548
    [Google Scholar]
  13. Evans L. A., McHugh T. M., Stites D. P., Levy J. A. 1987; Differential ability of HIV isolates to productively infect human cells. Journal of Immunology 138:3415–3418
    [Google Scholar]
  14. Fauci A. S. 1988; The human immunodeficiency virus: infectivity and mechanisms of pathogenesis. Science 239:617–622
    [Google Scholar]
  15. Fisher A. G., Ensoli B., Ivanoff L., Chamberlain M., Petteway S., Ratner L., Gallo R. C., Wong-Staal F. 1987; The sor gene of HIV-1 is required for efficient virus transmission in vitro. Science 237:888–893
    [Google Scholar]
  16. Gaines H., von Sydow M., Sonnerborg A., Albert J., Czajkawski I., Pehrson P. O., Chiodi F., Moberg L., Fenyo E. M., Åsjö B., Forsgren M. 1987; Antibody response in primary human immunodeficiency virus infection. Lancet ii:1249–1253
    [Google Scholar]
  17. Goto T., Ikuta K., Zhang J. J., Morita C., Sano K., Komatsu M., Fujita H., Kato S., Nakai M. 1990; The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1. Archives of Virology 111:87–101
    [Google Scholar]
  18. Goudsmit J., de Wolf F., Paul D. A., Epstein L. G., Lange J. M., Krone W. J., Speelman H., Wolters E. C., Van Der Noordaa J., Oleske J. M., van der Helm H. J., Coutinho R. A. 1986; Expression of human immunodeficiency virus antigen in serum and cerebrospinal fluid during acute and chronic infection. Lancet ii:177–180
    [Google Scholar]
  19. Ho D. D., Pomerantz R. J., Kaplan J. C. 1987; Pathogenesis of infection with human immunodeficiency virus. New England Journal of Medicine 317:278–286
    [Google Scholar]
  20. Ikuta K., Luftig R. B. 1986; Inhibition of cleavage of Moloney murine leukemia virus gag and env encoded precursor polyproteins by cerulenin. Virology 154:195–206
    [Google Scholar]
  21. Ikuta K., Imai H., Ueda S., Suehiro S., Yamamoto N., Kato S. 1987; Amplification of human immunodeficiency virus production from a virus-producing cell line in culture at high cell density. Japanese Journal of Cancer Research (Gann) 78:643–647
    [Google Scholar]
  22. Ikuta K., Morita C., Nakai M., Yamamoto N., Kato S. 1988; Defective human immunodeficiency virus (HIV) particles produced by cloned cells of HTLV-I-carrying MT-4 cells persistently infected with HIV. Japanese Journal of Cancer Research (Gann) 79:418–423
    [Google Scholar]
  23. Ikuta K., Morita C., Miyake S., Ito T., Okabayashi M., Sano K., Nakai M., Hirai K., Kato S. 1989; Expression of human immunodeficiency virus type I (HIV-1) antigens on the surface of a cell line persistently infected with HIV-1 that highly expresses HIV-1 antigens. Virology 170:408–417
    [Google Scholar]
  24. Imai H., Maotani-Imai K., Shin Y. S., Ikuta K., Suehiro S., Kurimura T., Kato S., Hirai K. 1991; A defective proviral DNA with a 2.6-kb deletion of human immunodeficiency virus type 1 (HIV-1) in a persistently HIV-1 infected cell clone. Virus Genes 5:81–88
    [Google Scholar]
  25. Jackson J. B., Kwok S. Y., Sninski J. J., Hopsicker J. S., Sannerud K. J., Rhame F. S., Henry K., Simpson M., Balfour H. H. Jr 1990; Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals. Journal of Clinical Microbiology 28:16–19
    [Google Scholar]
  26. Kikukawa R., Koyanagi Y., Harada S., Kobayashi N., Hatanaka M., Yamamoto N. 1986; Differential susceptibility to the acquired immunodeficiency syndrome retrovirus in cloned cells of human leukemic T-cell line Molt-4. Journal of Virology 57:1159–1162
    [Google Scholar]
  27. Kowalski M., Bergeron L., Dorfman T., Haseltine W., Sodroski J. 1991; Attenuation of human immunodeficiency virus type 1 cytopathic effect by a mutation affecting the transmembrane envelope glycoprotein. Journal of Virology 65:281–291
    [Google Scholar]
  28. McElrath M. J., Pruett J. E., Cohn Z. A. 1989; Mononuclear phagocytes of blood and bone marrow: comparative roles as viral reservoirs in human immunodeficiency virus type 1 infections. Proceedings of the National Academy of Sciences, U.S.A 86:675–679
    [Google Scholar]
  29. Minowada J., Ohnuma T., Moore G. E. 1972; Rosette forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. Journal of National Cancer Institute 49:891–895
    [Google Scholar]
  30. Miyoshi I., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y., Shiraishi Y., Akagi T. 1982; Type C virus-producing cell lines derived from adult T cell leukemia. In Adult T Cell Leukemia and Related Diseases, Gann Monograph of Cancer Research no 28 pp 219–228 Edited by Hanaoka M., Tatasuki K., Shimoyama M. Tokyo: Japan Scientific Societies Press; New York: Plenum Press;
    [Google Scholar]
  31. Morikawa Y., Overton H. A., Moore J. P., Wilkinson A. J., Brady R. L., Lewis S. J., Jones I. M. 1990; Expression of HIV-1 gp120 and human soluble CD4 by recombinant baculoviruses and their interaction in vitro. AIDS Research and Human Retroviruses 6:765–773
    [Google Scholar]
  32. Morita C., Ikuta K., Goto T., Sano K., Nakai M., Hirai K., Kato S. 1990; Isolation and characterization of cell clones persistently producing teardrop-shaped particles of human immunodeficiency virus type 1. Journal of Acquired Immune Deficiency Syndromes 3:231–237
    [Google Scholar]
  33. Nishino Y., Kishi M., Sumiya M., Ogawa K., Adachi A., Maotani-Imai K., Hirai K., Ikuta K. 1991; Human immunodeficiency virus type 1 vif vpr and vpu mutants can produce persistently infected cells. Archives of Virology (in press)
    [Google Scholar]
  34. Ogawa K., Shibata R., Kiyosasu T., Higuchi L., Kishida Y., Ishimoto A., Adachi A. 1989; Mutational analysis of the human immunodeficiency virus vpr open reading frame. Journal of Virology 63:4110–4114
    [Google Scholar]
  35. Paul D. A., Falk L. A., Kessler H. A., Chase R. M., Blaauw B., Chudwin D. S., Landay A. L. 1987; Correlation of serum HIVLess cytopathic HIV-1 antigen and antibody with clinical status in HIV-infected patients. Journal of Medical Virology 22:357–363
    [Google Scholar]
  36. Popovic M., Sarngadharan M. G., Read E., Gallo R. C. 1984; Detection, isolation and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224:497–500
    [Google Scholar]
  37. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  38. Sodroski J., Goh W. C., Rosen C., Tartar A., Portetelle D., Burny A., Haseltine W. 1986; Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions. Science 231:1549–1553
    [Google Scholar]
  39. Somasundaran M., Robinson H. L. 1987; A major mechanism of human immunodeficiency virus-induced cell killing does not involve cell fusion. Journal of Virology 61:3114–3119
    [Google Scholar]
  40. Spire B., Sire J., Zachar Y., Rey F., Barré-Sinoussi F., Galibert F., Hampe A., Chermann J. C. 1989; Nucleotide sequence of HIV-NDK: a highly cytopathic strain of the human immunodeficiency virus. Gene 81:275–284
    [Google Scholar]
  41. Tersmette M., de Goede R. E. Y., Al B. J. M., Winkel I. N., Gruters R. A., Cuypers H. T., Huisman H. G., Miedema F. 1988; Differential syncytium-inducing capacity of human immunodeficiency virus isolates: frequent detection of syncytium-inducing isolates in patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex. Journal of Virology 62:2026–2032
    [Google Scholar]
  42. Terwilliger E. F., Cohen E. A., Lu Y., Sodroski J. G., Haseltine W. A. 1989; Functional role of human immunodeficiency virus type 1 vpu. Proceedings of the National Academy of Sciences, U.S.A 86:675–679
    [Google Scholar]
  43. Yunoki M., Maotani-Imai K., Kusuda H., Motoyama M., Miyake S., Imai H., Shin Y. S., Kato S., Sano K., Morita C., Nakai M., Hirai K., Ikuta K. 1991; Production of infectious particles from defective human immunodeficiency virus type 1 (HIV-1)-producing cell clones by superinfection with infectious HIV-1. Archives of Virology 116:143–158
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-1-77
Loading
/content/journal/jgv/10.1099/0022-1317-73-1-77
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error